Genome-wide analysis and its application in Chinese hamster ovary cells
| Project/Area Number |
20360373
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Osaka University |
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Principal Investigator |
OMASA Takeshi Osaka University, 大学院・ソシオテクノサイエンス研究部, 教授 (00252586)
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| Co-Investigator(Kenkyū-buntansha) |
SHIRAI Akihiro 徳島大学, 大学院・ソシオテクノサイエンス研究部, 助教 (40380117)
OHTAKE Hisao 大阪大学, 大学院・工学研究科, 教授 (10127483)
HONDA Kohsuke 大阪大学, 大学院・工学研究科, 准教授 (90403162)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | CHO細胞 / ゲノム / BACライブラリー / 染色体 / gene amplification / BAC library / Chinese hamster ovary cell / chromosome |
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| Research Abstract |
Chinese hamster ovary (CHO) cells are the most dependable host cells for the industrial production of therapeutic proteins. We constructed CHO genomic bacterial artificial chromosome (BAC) library from a gene-amplified CHO cell line for genome-wide analysis of CHO cell lines. CHO chromosomal markers were constructed by fluorescence in situ hybridization (FISH) imaging using BAC clones as hybridization probes (BAC-FISH). Using constructed tools, we could analyze chromosome rearrangement in CHO cell line.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Glycosylation pattern of humanized IgG-like bispecific antibody produced by recombinant CHO cells2010
Author(s)
Wook-Dong Kim, Miwako Tokunaga, Hiroyuki Ozaki, Takuya Ishibashi, Kohsuke Honda, Hiroyuki Kajiura, Kazuhito Fujiyama, Ryutaro Asano, Tzumi Kumagai, Takeshi Omasa, Hisao Ohtake
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Journal Title
Applied Microbiology and Biotechnology Volume 85
Pages: 535-542
Related Report
Peer Reviewed
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[Journal Article] Bacterial artificial chromosome library for genome-wide analysis of Chinese hamster ovary cells2009
Author(s)
Takeshi Omasa, Yihua Cao,, Joon Young Park, Yasuhiro Takagi, Shuichi Kimura, Hidenori Yano, Kohsuke Honda, Shuichi Asakawa, Nobuyoshi Shimizu, Hisao Ohtake
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Journal Title
Biotechnology and Bioengineering Volume 104, No.5
Pages: 986-994
NAID
Related Report
Peer Reviewed
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[Presentation] Chinese Hamster Ovary Cells BAC library : Application to the characterization of CRC chromosomes2008
Author(s)
Takeshi Omasa, Yihua Cao, Shuiichi Kimura, S.M.A. Haghparast, Yasuhiro Takagi, Kohsuke Ronda, Hisao Ohtake
Organizer
In ; The thirteenth international biotechnology symposium and exhibition (IBS2008), IL-116, Journal of Biotechnology, vol.136S, p. S141
Place of Presentation
Dalian, China
Year and Date
2008-10-16
Related Report
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[Presentation] Analysis of the specific chromosomal region adjacent to the DHFR gene amplied in CHO DR 1000L-4N cell line2008
Author(s)
Takeshi Omasa, Yihua Cao, Joon-Young Park, Yasuhiro Takagi, Hidenori Yano, Kohsuke Honda, Shuichi Asakawa, Nobuyoshi Shimizu, Hisao Ohtake
Organizer
In ; Engineering Conference International : Cell Culture Engineering XI, 1.B.11, p.105
Place of Presentation
Sunshine Coast, Quennsland, Australia
Year and Date
2008-04-14
Related Report
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