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Elucidation and regulation of signal-propagation mechanisms in receptor proteins

Research Project

Project/Area Number 20370059
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionKyoto University

Principal Investigator

IMAMOTO Yasushi  Kyoto University, 大学院・理学研究科, 准教授 (80263200)

Co-Investigator(Kenkyū-buntansha) SHICHIDA Yoshinori  京都大学, 大学院・理学研究科, 教授 (60127090)
YAMASHITA Takahiro  京都大学, 大学院・理学研究科, 助教 (50378535)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000)
Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2008: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Keywordsロドプシン / PYP / 構造変化 / 蛋白質 / 活性化 / アゾベンゼン / フォトクロミズム / 蛋白質工学 / ペプチド / αヘリックス / 立体構造 / G蛋白質共役型受容体
Research Abstract

Proteins change their conformations in response to various kinds of stimuli. To reveal the intramolecular interaction that induces the protein conformational change, the amino acid residues which are responsible for the protein conformational changes of photoreceptor proteins in bacteria and animal retina were identified. Based on these findings, the protein conformation was mechanically controlled by a linker whose length is changed by photo-isomerization to regulate the activity of the protein.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (28 results)

All 2010 2009 2008 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (10 results) Remarks (1 results)

  • [Journal Article] Functional analysis of the second extracellular loop of rhodopsin by characterizing split variants.2010

    • Author(s)
      Sakai, K., Imamoto, Y., Yamashita, T., Shichida, Y.
    • Journal Title

      Photochem. Photobiol. Sci 9

      Pages: 1490-1497

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vertebrate rhodopsin adaptation to dim light via rapid meta-II intermediate formation.2010

    • Author(s)
      Sugawara, T., Imai, H., Nikaido, M., Imamoto, Y., Okada, N.
    • Journal Title

      Mol.Biol.Evol. 27

      Pages: 506-519

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of the second extracellular loop of rhodopsin by characterizing split variants2010

    • Author(s)
      Sakai, K., et al.
    • Journal Title

      Photochem.Photobiol.Sci.

      Volume: 9 Pages: 1490-1497

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vertebrate rhodopsin adaptation to dim light via rapid meta-II intermediate formation2010

    • Author(s)
      Sugawara, T.
    • Journal Title

      Mol.Biol.Evol. 27

      Pages: 506-519

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural effects on the ultrafast photoisomerization of photoactive yellow protein. ransient absorption spectroscopy of two point mutants.2009

    • Author(s)
      Changenet-Barret. P., Plaza, P., Martin, M.M., Chosrowjan, H., Taniguchi, S., Mataga, N., Imamoto, Y., Kataoka, M.
    • Journal Title

      J.Phys.Chem. C113

      Pages: 11605-11613

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] G protein subtype specificity of rhodopsin intermediates metarhodopsin Ib and metarhodopsin II.2009

    • Author(s)
      Morizumi, T., Kimata, N., Terakita, A., Imamoto, Y., Yamashita, T., Shichida, Y.
    • Journal Title

      Photochem. Photobiol. 85

      Pages: 57-62

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural effects on the ultrafast photoisomerization of photoactive yellow protein. Transient absorption spectroscopy of two point mutants2009

    • Author(s)
      Changenet-Barret. P.
    • Journal Title

      J.Phys.Chem.C 113

      Pages: 11605-11613

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] G protein subtype specificity of rhodopsin intermediates metarhodopsin Ib and metarhodopsin II.2009

    • Author(s)
      Morizumi T, Kimata N, Terakita A, Imamoto Y, Yamashita T, Shichida Y.
    • Journal Title

      Photochem Photobiol. 85(1)

      Pages: 57-62

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interaction between N-terminal loop and β-scaffold of photoactive yellow protein.2008

    • Author(s)
      Harigai, M., Kataoka, M., Imamoto, Y.
    • Journal Title

      Photochem. Photobiol. 84

      Pages: 1031-1037

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Low-temperature spectroscopy of Met100Ala mutant of photoactive yellow protein.2008

    • Author(s)
      Imamoto, Y., Harigai, M., Morimoto, T., Kataoka, M.
    • Journal Title

      Photochem. Photobiol. 84

      Pages: 970-976

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Thermal recovery of iodopsin from the photobleaching intermediates.2008

    • Author(s)
      Imamoto, Y., Shichida, Y.
    • Journal Title

      Photochem. Photobiol. 84

      Pages: 941-948

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The photoreaction of the photoactive yellow protein domain in the light sensor histidine kinase Ppr is influenced by the C-terminal domains.2008

    • Author(s)
      Kamikubo, H., Koyama, T., Hayashi, M., Shirai, K., Yamazaki, Y., Imamoto, Y., Kataoka, M.
    • Journal Title

      Photochem. Photobiol. 84

      Pages: 895-902

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interaction between N-terminal loop and beta-scaffold of photoacti veyellow protein.2008

    • Author(s)
      Harigai M, Kataoka M, Imamoto Y.
    • Journal Title

      Photochem Photobiol. 84(4)

      Pages: 1031-1037

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thermal recovery of iodopsin from photobleaching intermediates.2008

    • Author(s)
      Imamoto Y, Shichida Y.
    • Journal Title

      Photochem Photobiol. 84(4)

      Pages: 941-948

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Low-temperature spectroscopy of Met100Ala mutant of photoactive yellow protein.2008

    • Author(s)
      Imamoto Y, Harigai M, Morimoto T, Kataoka M.
    • Journal Title

      Photochem Photobiol. 84(4)

      Pages: 970-976

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The photoreaction of the photoactive yellow protein domain in the light sensor histidine kinase Ppr is influenced bv the C-terminal2008

    • Author(s)
      Kamikubo H, Koyama T, Hayashi M, Shirai K. Yamazaki Y, Imamoto Y
    • Journal Title

      Photochem Photobiol. 84(4)

      Pages: 895-902

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diverse roles of glycine residues conserved in photoactive yellow proteins.2008

    • Author(s)
      Imamoto Y, Tatsumi S, Harigai M, Yamazaki Y, Kamikubo H, Kataoka M.
    • Journal Title

      Biophys J. 94(9)

      Pages: 3620-3628

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 光センサー蛋白質の物性と機能メカニズム2010

    • Author(s)
      今元泰
    • Organizer
      生命機能研究科研究交流会「FBSコロキウム」
    • Place of Presentation
      大阪大学生命機能研究科
    • Year and Date
      2010-12-15
    • Related Report
      2010 Final Research Report
  • [Presentation] 光センサー蛋白質の物性と機能メカニズム2010

    • Author(s)
      今元泰
    • Organizer
      生命機能研究科研究交流会「FBSコロキウム」
    • Place of Presentation
      大阪大学(吹田市)
    • Year and Date
      2010-12-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Light-induced conformational change of photoactive yellow protein2009

    • Author(s)
      Imamoto, Y.
    • Organizer
      Memorial Symposium for the 25th International Prize for Biology "Biology of Sensing"
    • Place of Presentation
      Shiran Kaikan, Kyoto University, Kyoto, Japan
    • Year and Date
      2009-12-02
    • Related Report
      2010 Final Research Report
  • [Presentation] Light-induced conformational change of photoactive yellow protein2009

    • Author(s)
      Imamoto, Y.
    • Organizer
      Memorial Symposium for the 25th International Prize for Biology
    • Place of Presentation
      Shiran Kaikan, Kyoto University
    • Year and Date
      2009-12-02
    • Related Report
      2009 Annual Research Report
  • [Presentation] 蛋白質の構造変化:構造から予測される反応と実際の反応2008

    • Author(s)
      今元泰
    • Organizer
      日本生物物理学会第46回年会シンポジウム「タンパク質の構造に秘められた動作原理を読み取る」
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-04
    • Related Report
      2010 Final Research Report
  • [Presentation] 蛋白質の構造変化 : 構造から予測される反応と実際の反応2008

    • Author(s)
      今元泰
    • Organizer
      日本生物物理学会第46回年会シンポジウム「タンパク質の構造に秘められた動作原理を読み取る」
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] Association of rhodopsin and regulatory protein.2008

    • Author(s)
      今元泰
    • Organizer
      The 4th Asia and Oceania Conference on Photobiology, Swatantrata Bhawan, Banaras Hindu University
    • Place of Presentation
      Varanasi, India,
    • Year and Date
      2008-11-25
    • Related Report
      2010 Final Research Report
  • [Presentation] Association of rhodopsin and regulatory protein.2008

    • Author(s)
      Imamoto, Y.
    • Organizer
      The 4th Asia and Oceania Conference on Photobiology
    • Place of Presentation
      Banaras Hindu University, India
    • Year and Date
      2008-11-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] 蛋白質構造変化の伝播メカニズム「生物における光情報変換の一般性と多様性」2008

    • Author(s)
      今元泰
    • Organizer
      大阪大学蛋白質研究所セミナー
    • Place of Presentation
      大阪大学蛋白質研究所
    • Year and Date
      2008-04-18
    • Related Report
      2010 Final Research Report
  • [Presentation] 蛋白質構造変化の伝播メカニズム20. Imamoto, Y. "Association of rhodopsin and regulatory2008

    • Author(s)
      今元泰
    • Organizer
      大阪大学蛋白質研究所セミナー「生物における光情報変換の一般性と多様性」
    • Place of Presentation
      大阪大学蛋白質研究所
    • Year and Date
      2008-04-18
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://photo1.biophys.kyoto-u.ac.jp/

    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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