Elucidation of protein dynamics as the control of protein function
| Project/Area Number |
20370062
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
KATAOKA Mikio Nara Institute of Science and Technology, 物質創成科学研究科, 教授 (30150254)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIBKUBO Hironari 奈良先端科学技術大学院大学, 物質創成科学研究科, 准教授 (20311128)
YAMAZAKI Yoichi 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (40332770)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAGAWA Hiroshi 日本原子力研究開発機構, 量子ビーム応用研究部門, 研究員 (20379598)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2008: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Keywords | 中性子散乱 / 蛋白質動力学 / 誘導折り畳み / 水和 / 動力学転移 / 動力学的転移 / 非天然構造 / 核酸分解酵素 / 中性子非干渉性散乱 / 平均二乗変位 / 水和水 / 動力学の階層性 / タンパク質動力学 / ボソンピーク / 中性子非弾性散乱 / 中性子準弾性散乱 / 振動スペクトル |
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| Research Abstract |
We succeeded to create an functionally active mutant without native structure under physiological condition. Using the mutant and wild type, neutron incoherent scattering experiments were performed under various hydration levels to reveal the protein dynamics required for function. Protein dynamics relevant for function is controlled by hydration water, and the hierarchical structures of protein dynamics are dependent on the conformational states. The essential property of hydration water is revealed to be the formation of hydrogen bond network between hydration water molecules.
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Report
(4 results)
Research Products
(118 results)
