| Budget Amount *help |
¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
Fiscal Year 2010: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2008: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Research Abstract |
Based on microarray analysis combined with molecular genetics, we revealed that more than one third of genes on the genome exhibit significant circadian transcription rhythms with peaking at either subjective dawn or dusk under continuous light conditions. When cells were transferred to the dark, most of gene expression was dramatically suppressed and gave rise to serious discrepancy between transcriptomic and proteomic profiles. Moreover, we found less than 8% of genes on the genome were induced even in the dark, and most of them were under the control of the Kai proteins. Since the kai gene mRNAs were rapidly lost in the dark, our observation revealed the first example of clock-controlled transcription without de novo clock gene expression.
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