Development of DNA markers and QTL mapping relating to oleic acid contents in peanut.

• Project/Area Number: 20380008
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Kazusa DNA Research Institute
• Principal Investigator: TABATA Satoshi Kazusa DNA Research Institute, 副所長 (70197549)
• Co-Investigator(Kenkyū-buntansha): ISOBE Sachiko (財)かずさDNA研究所, 植物ゲノム研究部, 主任研究員 (20343973) ; AOKI Koh (財)かずさDNA研究所, 生体機能応用研究室, 室長 (30344021)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2008: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 植物 / 育種 / ゲノム / 遺伝学 / 脂質 / 落花生 / 脂肪酸 / DNAマーカー / オレイン酸 / 連鎖地図 / SNP / FAD遺伝子

Research Abstract

In order to accelerate breeding for high oleic acid contents in peanut, two selection markers were developed from candidate gene sequences controlling oleic acid contents. In addition, genome wide polymorphic DNA markers (microsatellite and transposon element markers) were developed and mapped on genetic linkage maps of peanut. Those genome wide DNA markers as well as the two candidate gene markers were used for marker assisted selection of the high-oleic peanut breeding program. As a result, we succeed to reduce breeding periods from five to three years.

Research Products

• [Journal Article] Large-scale development of EST-derived SSR markers and diversity analysis in Arachis spp. (2011)
  • Author(s): Koilkonda P, Sato S, Satoshi Tabata, Shirasawa K, Hirakawa H, Sakai H, Sasamoto S, Watanabe A, Wada T, Kishida Y, Tsuruoka H, Fujishiro T, Yamada M, Kohara M, Suzuki S, Hasegawa M, Kiyoshima H, Isobe S
  • Journal Title: Molecular Breeding
  • Peer Reviewed
• [Journal Article] Large-scale development of EST-derived SSR markers and diersity analysis in Arachis spp.
  • Author(s): Padmalatha Koilkonda, Shusei Sato, Satoshi Tabata, Kenta Shirasawa, Hideki Hirakawa, Hiroe Sakai, Shigemi Sasamoto, Akiko Watanabe, Tsuyuko Wada, Yoshie Kishida, Hisano Tsuruoka, Tsunakazu Fujishiro, Manabu Yamada, Mitsuyo Kohara, Shigeru Suzuki, Makoto Hasegawa, Hiroyuki Kiyoshima, Sachiko Isobe
  • Journal Title: Molecular Breeding Volume: (Accepted)
  • Peer Reviewed
• [Presentation] Getting across the Devil river in the Legume land (2010)
  • Author(s): Sachiko Isobe, Kenta Shirasawa, Shusei Sato, Hideki Hirakawa, Padmalatha Koilkonda, Satoshi Tabata
  • Organizer: 国際マメ科植物遺伝・ゲノム学会(ICLGG)
  • Place of Presentation: Asilomar, CA, USA
  • Year and Date: 2010-07-05
• [Presentation] ラッカセイに見出したMITE型トランスポゾンの特徴 (2010)
  • Author(s): 白澤健太,Koilkonda P,田畑哲之,磯部祥子
  • Organizer: 育種学研究(12(別2):282)
• [Presentation] Development of microsatellite, single nucleotide polymorphism, and transposon markers for peanut breeding. (2009)
  • Author(s): Shirasawa K, Koilkonda P, Naito Y, Watanabe M, Suzuki S, Hasegawa H, Kiyoshima H, Kuwata C, Tabata S, Isobe
  • Organizer: Advances in Arachis through Genomics & Biotechnology-2009
  • Place of Presentation: Bamako, Mali
• [Presentation] Marker assisted backcrossing selection (MABS) for oleic acid content in peanut. (2009)
  • Author(s): Shirasawa K, Koilkonda P, Naito Y, Watanabe M, Suzuki S, Hasegawa H, Kiyoshima H, Kuwata C, Tabata S, Isobe S
  • Organizer: International Plant Molecular Breeding Congress.
  • Place of Presentation: USA
• [Presentation] Development of microsatellite, single nucleotide polymorphism, and transposon markers for peanut breeding (2009)
  • Author(s): Shirasawa, Isobe etal.
  • Organizer: Advances in Arachis through Genomics & Biotechnology-2009
  • Place of Presentation: マリ共和国 Bamako
• [Presentation] Marker assisted backcrossing selection(MABS)for oleic acid content in peanut (2009)
  • Author(s): Shirasawa, Isobe etal.
  • Organizer: International plant Molecular Breeding Congress
  • Place of Presentation: アメリカ合衆国・セントルイス
• [Presentation] Development of microsatellite, SNP and AFLP markers in cultivated peanut. (2008)
  • Author(s): Isobe S, Sato S, Shirasawa K, Suzuki S, Watanabe M, Tabata S.
  • Organizer: Third international conference of the Peanut Research Community on Advances in Arachis through Genomics and Biotechnology (AAGB-2008), ICRISAT
  • Place of Presentation: Hederabad India
• [Presentation] Development of microsatellite, SNP and AFLP markers in cultivated peanut (2008)
  • Author(s): Isobe S, Sato S, Shirasawa K, Suzuki S, Watanabe M, Tabata S
  • Organizer: 3rd international conference of the Peanut Research Community on Advances in Arachis through Genomics and Biotechnology
  • Place of Presentation: ICRISAT(ハイデラバード、インド)
• [Presentation] ラッカセイに見出したMITE型トランスポゾンの特徴
  • Author(s): 白澤健太, Koilkonda P, 田畑哲之, 磯部祥子
  • Organizer: 育種学会 育種学研究12(別2):282
  • Place of Presentation: 横浜市立大学(震災のため講演はキャンセル)
• [Remarks] ホームページ等
• [Remarks] 落花生のDNAマーカーに関するデータベース
  • URL: http://marker.kazusa.or.jp/peanut
• [Remarks] 落花生のEST-SSRマーカーに関するデータベース
  • URL: http://marker_kazusa.or.jp/peanut