| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Research Abstract |
We screened ripening key genes using tomato DNA macroarray consisted of 12, 000 clones, ripening-impaired mutants and transgenic technology. Ripening-regulated 428 genes were isolated and 244 genes were regulated under ethylene signaling. As ripening-regulated novel transcription factor or signaling factor, GRAS, SAHH, b-Zip and BTB genes were identified, suggesting their involvement in regulation of fruit ripening at the downstream of ethylene signaling. Transgenic tomatoes with all known LeEILs suppressed, exhibited non-ripening phenotype and indicated that onset of ripening-ethylene is regulated by developmental factors. Based on information in tomato, low-temperature induced ripening in kiwi fruit was analyzed and it was shown to be independent of ethylene signaling.
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