| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2009: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2008: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Research Abstract |
A virus-induced gene silencing (VIGS) system using Apple latent spherical virus (ALSV) vectors was established in legume, cucurbit, Solanaceae species, and Rosaceae fruit trees. ALSV vectors could be successfully used to silence disease resistant N gene in tobacco and RCY1 gene in Arabidopsis thaliana. Infection with an ALSV vector having a fragment of soybean isoflavone synthase 2 gene also led to a reduction of the levels of an isoflavone content in the cotyledons of infected seeds.
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