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Analysis of intracellular movement of plant DNA viruses

Research Project

Project/Area Number 20380026
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant pathology
Research InstitutionThe University of Tokyo

Principal Investigator

UGAKI Masashi  東京大学, 大学院・新領域創成科学研究科, 教授 (20323438)

Project Period (FY) 2008 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
KeywordsDNAウイルス / 細胞内移行 / 細胞骨格 / ジェミニウイルス / カリモウイルス / 細胞質移行 / 植物ウイルス
Research Abstract

After entry into the host plant cell, plant DNA viruses must move toward a nucleus where they express their genes and replicate. After replication, they must move toward plasmodesmata. We studied to see whether plant cytoskeletons are involved in the viral intracellular movement and found that the actin filament plays an important role in Cauliflower mosaic virus infection.

Report

(6 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (34 results)

All 2012 2011 2010 2009 2008 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (18 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Transgenic rice plants that over-express the mannose-binding rice lectin have enhanced resistande to rice blast2012

    • Author(s)
      Shinjo A, Araki Y, Hirano T, Ugaki M, Teraoka T
    • Journal Title

      Journal of General Plant Pathology

      Volume: 77 Issue: 2 Pages: 85-92

    • DOI

      10.1007/s10327-010-0290-x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transgenic rice plants that over-express the mannose-binding rice lectin have enhanced resistance to rice blast2011

    • Author(s)
      Shinjo, A., Araki, Y., Hirano, K., Arie, T., Ugaki, M. and Teraoka T.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 77 Pages: 85-92

    • NAID

      10029720736

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pseudo-polyprotein translated from the full-length ORF1 of capillovirus is important for pathogenicity, but a truncated ORF1 protein without variable and CP regions is sufficient for replication2010

    • Author(s)
      Hirata, H., Yamaji, Y., Komatsu, K., Kagiwada, S., Oshima, K., Okano, Y., Takahashi, T., Ugaki, M. and Namba S.
    • Journal Title

      Virus Research

      Volume: 152 Pages: 1-9

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of rice RNA-dependent RNA polymerase 1(OsRDR1) in virus-mediated RNA silencing after particle bombardment2010

    • Author(s)
      Chen, H., Tamai, A., Mori, M., Ugaki, M., Tanaka, Y., Samadder, P. P., Miyao, A., Hirochika, H., Yamaoka, N., Nishiguchi M.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 76 Pages: 152-160

    • NAID

      10029719442

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pseudo-polyprotein translated from the full-length ORF1 of capillovirus is important for pathogenicity, but a truncated ORF1 protein without variable and CP regions is sufficient for replication.2010

    • Author(s)
      Hirata, H., Yamaji, Y., Komatsu, K., Kagiwada, S., Oshima, K., Okano, Y., Takahashi, T., Ugaki, M., Namba, S.
    • Journal Title

      Virus Research 152

      Pages: 1-9

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Analysis of rice RNA-dependent RNA polymerase 1 (OsRDR1) in virus-mediated RNA silencing after particle bombardment.2010

    • Author(s)
      Chen, H., Tamai, A., Mori, M., Ugaki, M., Tanaka, Y., Samadder, P.P., Miyao, A., Hirochika, H., Yamaoka, N., Nishiguchi, M.
    • Journal Title

      Journal of General Plant Pathology 76

      Pages: 152-160

    • NAID

      10029719442

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Pseudo-polyprotein translated from the full-length ORF1 of capillovirus is important for pathogenicity, but a truncated ORF1 protein without variable and CP regions is sufficient for replication.2010

    • Author(s)
      Hirata, H.Yamaji, Y., Komatsu, K., Kagiwada, S., Oshima, K., Okano, Y., Takahashi, T., Ugaki, M., Namba, S.
    • Journal Title

      Virus Research

      Volume: 152 Pages: 1-9

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of rice RNA-dependent RNA polymerase 1(OsRDR1)in virus-mediated RNA silencing after particle bombardment2010

    • Author(s)
      Chen, H., Tamai, A., Mori, M., Ugaki, M., Tanaka, Y., Samadder, PP., Miyao, A., Hirochika, H., Yamaoka, N., Nishiguchi, M.
    • Journal Title

      Journal of General Plant Pathology 76

      Pages: 152-160

    • NAID

      10029719442

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Agrobacterium-mediated transformation of spinach(Spinacia oleracea) with Bacillus thuringiensis cry1Ac gene for resistance against two common vegetable pests2009

    • Author(s)
      Bao, J. H., Chin, D. P., Fukami, M., Ugaki, M., Nomura, M. and Mii M.
    • Journal Title

      Plant Biotechnology

      Volume: 26 Pages: 249-254

    • NAID

      10025182014

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Peanut stunt virus 2b cistron plays a role in viral local and systemic accumulation and virulence in Nicotiana benthamiana2008

    • Author(s)
      Netsu, O., Hirastuka, K., Kuwata, S., Hibi, T., Ugaki, M. and Suzuki M.
    • Journal Title

      Archives of Virology

      Volume: 153 Pages: 1731-1735

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Peanut stunt virus 2b cistron plays a role in viral local and systemic accumulation and virulence in Nicotiana benthamiana.2008

    • Author(s)
      Netsu, O., Hirastuka, K., Kuwata, S., Hibi, T., Ugaki, M., Suzuki, M.
    • Journal Title

      Archives of Virology 153

      Pages: 1731-1735

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Peanut stunt virus 2b cistron plays a role in viral local and systemic accumulation and virulence in Nicotiana benthamiana.2008

    • Author(s)
      Netsu, O., Hirastuka, K., Kuwata, S., Hibi, T., Ugaki, M. and Suzuki, M.
    • Journal Title

      Archives of Virology 153

      Pages: 1731-1735

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Beet severe curly top virusのC1はウイルス鎖遺伝子の転写活性化タンパク質である2012

    • Author(s)
      早川豪人、平塚和之、鈴木匡、宇垣正志
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] トマト黄化葉巻ウイルスを吸汁させたタバココナジラミ中腸円筒細胞の細胞内微細構造2012

    • Author(s)
      内堀美和、鈴木匡、宇垣正志
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] トマト黄化葉巻ウイルスを吸汁させたタバココナジラミ中腸円筒細胞の細胞内微細構造2012

    • Author(s)
      内堀美和、鈴木匡、宇垣正志
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際センター,福岡
    • Related Report
      2011 Final Research Report
  • [Presentation] Beet severe curly top virusのC1はウイルス鎖遺伝子の転写活性化タンパク質である2012

    • Author(s)
      早川豪人、平塚和之、鈴木匡、宇垣正志
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡国際センター,福岡
    • Related Report
      2011 Final Research Report
  • [Presentation] Identification of the protein which activates the expression of the virus-sense genes of Beet severe curly top virus2011

    • Author(s)
      Hayakawa H, Hiratsuka K, Suzuki M, Ugaki M
    • Organizer
      XV International Comgress of Virology
    • Place of Presentation
      Sapporo Convention center (Sapporo, Japan)
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Beet severe curly top virusのウイルス鎖遺伝子の発現活性化に関与するタンパク質の同定2011

    • Author(s)
      早川豪人、鈴木匡、宇垣正志
    • Organizer
      平成23年度日本植物病理学会大会講演要旨集p.165
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-03-28
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] Beet severe curly topvirusのウイルス鎖遺伝子の発現活性化に関与するタンパク質の同定2011

    • Author(s)
      早川豪人、鈴木匡、宇垣正志
    • Organizer
      平成23年度日本植物病理学会大会講演要旨集p.165
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification of the protein which activates the expression of the virus-sense genes of Beet severe curly top virus2011

    • Author(s)
      Hayakawa, H., Hiratsuka, K., Suzuki, M. and Ugaki, M
    • Organizer
      XV International Congress of Virology
    • Place of Presentation
      Sapporo, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Beet severe curly top virusのウイルス鎖遺伝子の発現活性化に関与するタンパク質の同定2011

    • Author(s)
      早川豪人、鈴木匡、宇垣正志
    • Organizer
      平成23年度日本植物病理学会大会講演要旨集
    • Place of Presentation
      東京農工大学,東京
    • Related Report
      2011 Final Research Report
  • [Presentation] Beet severe curly top virusの感染性クローンの構築と感染葉からの欠損DNAの検出2010

    • Author(s)
      寺田良介、川島祐介、小野里歩、鈴木匡、宇垣正志
    • Organizer
      平成22年度日本植物病理学会大会講演要旨集p.152
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2010-04-19
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] Beetsevere curly top virusの感染性クローンの構築と感染葉からの欠損DNAの検出2010

    • Author(s)
      寺田良介、川島祐介、小野里歩、鈴木匡、宇垣正志
    • Organizer
      平成22年度日本植物病理学会大会講演要旨集p.152
    • Place of Presentation
      国立夢都国際会館
    • Year and Date
      2010-04-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] Beet severe curly top virusの感染性クローンの構築と感染葉からの欠損DNAの検出2010

    • Author(s)
      寺田良介、川島祐介、小野里歩、鈴木匡、宇垣正志
    • Organizer
      平成22年度日本植物病理学会大会講演要旨集
    • Place of Presentation
      京都国際会館,京都
    • Related Report
      2011 Final Research Report
  • [Presentation] Phylogenetic analysis of Tomato yellow leaf curl isolates occurring in central Japan2008

    • Author(s)
      Ueno, T., Suzuki, M., Ugaki, M.
    • Organizer
      XIV International Congress of Virology
    • Place of Presentation
      Istanbul Convention and Exhibition Center, Istanbul, Turkey
    • Year and Date
      2008-08-14
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] Phylogene2b protein of Peanut stunt virus is important for local and systemic accumulation.2008

    • Author(s)
      Netsu, O., Kuwata, S., Hibi, T., Ugaki, M., Suzuki, M.
    • Organizer
      XIV International Congress of Virology
    • Place of Presentation
      Istanbul Convention and Exhibition Center, Istanbul, Turkey
    • Year and Date
      2008-08-14
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] 2b protein of Peanut stunt virus is important for local and Systemic accumulation2008

    • Author(s)
      Netsu, O., Kuwata, S., Hibi, T., Ugaki, M. and Suzuki, M.
    • Organizer
      XIV International Congress of Virology
    • Place of Presentation
      Istanbul, Turkey
    • Year and Date
      2008-08-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] Phylogenetic analysis of Tomato yellow leaf curl virus isolates occurring in central Japan2008

    • Author(s)
      Ueno, T., Suzuki, M. and Ugaki, M.
    • Organizer
      XIV International Congress of Virology
    • Place of Presentation
      Istanbul, Turkey
    • Year and Date
      2008-08-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] M. 2b protein of Peanut stunt virus is important for local and systemic accumulation2008

    • Author(s)
      Netsu, O., Kuwata, S., Hibi, T., Ugaki, M. and Suzuki
    • Organizer
      XIV International Congress of Virology
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2011 Final Research Report
  • [Presentation] Phylogenetic analysis of Tomato yellow leaf curl virus isolates occuring in central Japan2008

    • Author(s)
      Ueno, T., Suzuki, M. and Ugaki, M.
    • Organizer
      XIV International Congress of Virology
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2011 Final Research Report
  • [Book] 植物病理学(遺伝子組換え法による抵抗性育種)(眞山滋志・難波成任編)2010

    • Author(s)
      宇垣正志
    • Publisher
      文永堂
    • Related Report
      2010 Self-evaluation Report
  • [Book] 植物病理学2009

    • Author(s)
      宇垣正志, 他
    • Total Pages
      328
    • Publisher
      文永堂出版
    • Related Report
      2011 Final Research Report
  • [Book] 植物病理学(眞山、難波編)2009

    • Author(s)
      宇垣正志, 他
    • Total Pages
      328
    • Publisher
      文永堂出版
    • Related Report
      2009 Annual Research Report
  • [Remarks] 日本植物病理学会学生優秀発表賞早川豪人、平塚和之、鈴木匡、宇垣正志. Beet severe curly top virusのC1はウイルス鎖遺伝子の転写活性化タンパク質である.平成24年度日本植物病理学会大会. 2012年3月.福岡国際センター,福岡.

    • Related Report
      2011 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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