Molecular mechanisms of protein synthesis shutdown in insect cells infected with nucleopolyhedrovirus
| Project/Area Number |
20380034
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Nagoya University |
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Principal Investigator |
IKEDA Motoko Nagoya University, 大学院・生命農学研究科, 准教授 (20262892)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Michihiro 名古屋大学, 大学院・生命農学研究科, 教授 (60111837)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2008: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Keywords | 核多角体病ウイルス / 昆虫細胞 / 抗ウイルス応答 / リボソームRNA分解 / 全タンパク質合成停止 / カイコ培養細胞 / 生体防御機構 / p143遺伝子 / 昆虫培養細胞 |
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| Research Abstract |
The molecular basis for the global protein synthesis shutdown in Bombyx mori BM-N cells caused by several nucleopolyhedroviruses (NPVs) infections is not well known. In this study, we showed that the RNA in BM-N cells was degraded quickly after abortive infection of the cells by various NPVs. In contrast, the RNA degradation was not induced by Bombyx mori NPV infection which results in productive infection in the cells. These results indicate that a viral factor and/or its function induce the degradation of RNA, which causes global protein synthesis shutdown in BM-N cells.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Comparative genomic sequence analysis of novel Helicoverpa armigera nucleopolyhedrovirus (NPV) isolated from Kenya and three other previously sequenced Helicoverpa spp.NPVs.2009
Author(s)
Ogembo, J.G., Caoili, B.L., Shikata, M., Chaeychomsri, S., Kobayashi, M., Ikeda, M.
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Journal Title
Virus Gene 39
Pages: 261-272
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