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Cellulolytic systems by the symbiotic bacteria in termites

Research Project

Project/Area Number 20380037
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionUniversity of the Ryukyus

Principal Investigator

TOKUDA Gaku  琉球大学, 熱帯生物圏研究センター, 准教授 (90322750)

Project Period (FY) 2008 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2009: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2008: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Keywords昆虫利用 / 機能開発 / 応用動物 / 昆虫 / 酵素 / セルラーゼ / 共生細菌 / シロアリ / 後腸 / バクテリア / クロマトグラフィー
Research Abstract

Research areas in cellulose digestion have generally attracted many people as it could be converted to non-harmful bioethanol in a reasonable price. Termites digest plant biomass efficiently in both the midgut and the hindgut digestive systems, In the present study, the hindgut celluloytic systems were primarily analyzed. As a result, diverse genes relevant to lignocellulose decompositions and to carbohydrate-binding modules were obtained. Detailed analyses suggest that the termites are equipped with dual digestive systems, in which the midgut system hydrolyzes cellulose and the hindgut system is primarily involved in hemicellulose degradations.

Report

(6 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (55 results)

All 2012 2011 2010 2009 2008 Other

All Journal Article (23 results) (of which Peer Reviewed: 18 results) Presentation (23 results) Book (3 results) Remarks (6 results)

  • [Journal Article] Cellulolytic environment in the midgut of the wood-feeding higher termite Nasutitermes takasagoensis2012

    • Author(s)
      Tokuda, G., Watanabe, H., Hojo, M., Fujita, A., Makiya, H., Miyagi, M., Arakawa, G., Arioka, M
    • Journal Title

      Journal of Insect Physiology

      Volume: 58 Issue: 1 Pages: 147-154

    • DOI

      10.1016/j.jinsphys.2011.10.012

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genome shrinkage and loss of nutrient-providing potential in the obligate symbiont of the primitive termite Mastotermes darwiniensis2012

    • Author(s)
      Sabree, Z.L.
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 78 Issue: 1 Pages: 204-210

    • DOI

      10.1128/aem.06540-11

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cellulose digestion by termites and their symbionts2012

    • Author(s)
      Tokuda, G.
    • Journal Title

      Proceedings of the 9th Pacific-Rim Termite Research Group Conference

      Pages: 10-15

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Heterologous expression and characterization of a glucose-stimulated β-glucosidase from the termite Neotermes koshunensis in Aspergillus oryzae2011

    • Author(s)
      Uchima, C.A.
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 89 Pages: 1761-1771

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cellulolytic systems in insects2010

    • Author(s)
      Watanabe, H. & Tokuda, G
    • Journal Title

      Annual Review of Entomology

      Volume: 55 Issue: 1 Pages: 609-632

    • DOI

      10.1146/annurev-ento-112408-085319

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Purification and characterization of termite endogenous β-1,4-endoglucanases produced in Aspergillus orizae.2010

    • Author(s)
      Hirayama, K., Watanabe, H., Tokuda, G., Kitamoto, K., Arioka, M.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 74

      Pages: 1680-1686

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Cellulolytic systems in insects.2010

    • Author(s)
      Watanabe, H., Tokuda, G.
    • Journal Title

      Annual Review of Entomology 55

      Pages: 609-632

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Details of the digestive system in the midgut of Coptotermes formosanus Shiraki2010

    • Author(s)
      Fujita, A.
    • Journal Title

      Journal of Wood Science

      Volume: 56 Pages: 222-226

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification and characterization of termite endogenous β-1, 4-endoglucanases produced in Aspergillus orizae2010

    • Author(s)
      Hirayama, K.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 74 Pages: 1680-1686

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] タカサゴシロアリのセルロース消化について2010

    • Author(s)
      徳田岳
    • Journal Title

      しろあり

      Volume: 154 Pages: 37-39

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Cellulolytic systems in insects2010

    • Author(s)
      Watanabe, H.
    • Journal Title

      Annual Review of Entomology 55

      Pages: 609-632

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lignocellulose digestive system in termites and future perspective on its applications2010

    • Author(s)
      穂田岳
    • Journal Title

      第12回建設・環境マネジメント講演会論文集

      Pages: 41-79

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Digestiveβ-glucosidases from the wood-feeding higher termite2009

    • Author(s)
      Tokuda, G., Miyagi, M., Makiya, H., Watanabe, H. & Arakawa, G
    • Journal Title

      Insect Biochemistry and Molecular Biology

      Volume: 39 Issue: 12 Pages: 931-937

    • DOI

      10.1016/j.ibmb.2009.11.003

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Purification and Molecular Cloning of Xylanases from the Wood-Feeding Termite, Coptotermes formosanus Shiraki2009

    • Author(s)
      Arakawa, G., Watanabe, H., Yamasaki, H., Maekawa, H. & Tokuda, G
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 73 Issue: 3 Pages: 710-718

    • DOI

      10.1271/bbb.80788

    • NAID

      10027539061

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Termites as functional gene resources2009

    • Author(s)
      Matsui, T., Tokuda, G. & Shinzato, N
    • Journal Title

      Recent Patents on Biotechnology

      Volume: 3 Pages: 10-18

    • URL

      http://www.benthamdirect.org/pages/content.php?BIOT/2009/00000003/00000001/0002BIOT.SGM

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Digestive β-glucosidases from the wood-feeding higher termite, Nasutitermes takasagoensis : intestinal distribution, molecular characterization, and alteration in sites of expression.2009

    • Author(s)
      Tokuda, G., Miyagi, M., Makiya, H., Watanabe, H., Arakawa, G.
    • Journal Title

      Insect Biochemistry and Molecular Biology 39

      Pages: 931-937

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Purification and molecular cloning of xylanases from the wood-feeding termite, Coptotermes formosanus Shiraki.2009

    • Author(s)
      Arakawa, G., Watanabe, H., Yamasaki, H., Maekawa, H., Tokuda, G.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 73

      Pages: 710-718

    • NAID

      10027539061

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Termites as functional gene resources.2009

    • Author(s)
      Matsui, T., Tokuda, G., Shinzato, N.
    • Journal Title

      Recent Patents on Biotechnology 3

      Pages: 10-18

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Digestive β-glucosidases from the wood-feeding higher termite, Nasutitermes takasagoensis : intestinal distribution, molecular characterization, and alteration in sites of2009

    • Author(s)
      Tokuda, G.
    • Journal Title

      Insect Biochemistry and Molecular Biology 39

      Pages: 931-937

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reconstruction and mimicking of the termite midgut as a micro-scale biomass conversion system2009

    • Author(s)
      Watanabe, H.
    • Journal Title

      Biotechnology of Lignocellulose Degradation and Biomass Utilization

      Pages: 16-20

    • Related Report
      2009 Annual Research Report
  • [Journal Article] An overview of the cellulolytic system in the wood-feeding termite, Nasutitermes takasagoensis(Shiraki)2009

    • Author(s)
      Tokuda, G.
    • Journal Title

      Biotechnology of Lignocellulose Degradation and Biomass Utilization

      Pages: 310-313

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Termites as functional gene resources.2009

    • Author(s)
      Matsui, T.
    • Journal Title

      Recent Patents on Biotechnology 3

      Pages: 10-18

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification and molecular cloning of xylanases from the wood- feeding termite, Coptotermes formosanus Shiraki.2009

    • Author(s)
      Arakawa, G.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 73

      Pages: 710-718

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Cellulose digestion by termites and their symbionts2012

    • Author(s)
      Tokuda, G
    • Organizer
      The 9th Pacific-Rim Termite Research Group Conference
    • Place of Presentation
      Hanoi, Vietnam
    • Year and Date
      2012-02-27
    • Related Report
      2011 Final Research Report
  • [Presentation] Cellulose digestion by termites and their symbionts2012

    • Author(s)
      Tokuda, G.
    • Organizer
      The 9th Pacific-Rim Termite Research Group Conference
    • Place of Presentation
      Melia Hotel, Hanoi, Vietnam(招待講演)
    • Year and Date
      2012-02-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Lignocellulolytic systems in higher termites2011

    • Author(s)
      Tokuda, G
    • Organizer
      International Symposium on Digestive systems in Termites
    • Place of Presentation
      Okinawa, Japan
    • Year and Date
      2011-10-06
    • Related Report
      2011 Final Research Report
  • [Presentation] Lignocellulolytic systems in higher termites2011

    • Author(s)
      Tokuda, G.
    • Organizer
      International Symposium on Digestive systems in Termites
    • Place of Presentation
      琉球大学50周年記念館
    • Year and Date
      2011-10-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] タカサゴシロアリ後腸メタゲノム解析による細菌由来リグノセルロース分解酵素の多様性2011

    • Author(s)
      徳田岳
    • Organizer
      (社)日本動物学会第82回大会
    • Place of Presentation
      旭川市神楽地区公共施設
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] タカサゴシロアリ後腸メタゲノム解析による細菌由来リグノセルロース分解酵素の多様性2011

    • Author(s)
      徳田岳・齋藤星耕
    • Organizer
      (社)日本動物学会第82回大会
    • Place of Presentation
      旭川市神楽地区公共施設(旭川市)
    • Related Report
      2011 Final Research Report
  • [Presentation] タカサゴシロアリ中腸におけるセルロース消化環境2011

    • Author(s)
      徳田岳
    • Organizer
      第55回日本応用動物昆虫学会
    • Place of Presentation
      九州大学(福岡市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Lignocellulolytic systems in termites : how do endogenous and microbial enzymes participate in lignocelluloses breakdown2010

    • Author(s)
      Tokuda, G
    • Organizer
      Max Plank Institute Seminar Series in Molecular, Cellular, and Environmental Microbiology
    • Place of Presentation
      Marburg, Germany
    • Year and Date
      2010-08-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Lignocellulolytic systems in termites : how do endogenous and microbial enzymes participate in lignocelluloses breakdown?2010

    • Author(s)
      Tokuda, G.
    • Organizer
      Max Plank Institute Seminar Series in Molecular, Cellular, and Environmental Microbiology
    • Place of Presentation
      Marburg, Germany
    • Year and Date
      2010-08-16
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] Lignocellulolytic systems in termites : how do endogenous and microbial enzymes participate in lignocelluloses breakdown?2010

    • Author(s)
      Tokuda, G.
    • Organizer
      Max Plank Institute Seminar Series in Molecular, Cellular, and Environmental Microbiology
    • Place of Presentation
      マックスプランク研究所(ドイツ)
    • Year and Date
      2010-08-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Heterologous production and characterization of β-glucosidases from termites2010

    • Author(s)
      Uchima, C.A.
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学(東京都目黒区)
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] 大腸菌によるタカサゴシロアリβ-グルコシダーゼ発現系の構築とポリクローナル抗体の作製2010

    • Author(s)
      徳田岳
    • Organizer
      第54回日本応用動物昆虫学会大会
    • Place of Presentation
      千葉大学(千葉県千葉市)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Genetic diversity of the Formosan subterranean termite, Coptotermes fromosanus Shiraki in relation to the distribution of staphylinid termitophiles2010

    • Author(s)
      Yamada, A.
    • Organizer
      Seventh Conference of the Pacific Rim Termite Research Group
    • Place of Presentation
      Quality Hotel Singapore(Singapore)
    • Year and Date
      2010-03-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] シロアリ類によるリグノセルロース消化機構と応用への展望2010

    • Author(s)
      徳田岳
    • Organizer
      第12回建設・環境マネジメント講演会
    • Place of Presentation
      山口大学(山口市)
    • Year and Date
      2010-02-19
    • Related Report
      2011 Final Research Report 2010 Self-evaluation Report
  • [Presentation] シロアリ類によるリグノセルロース消化機構と応用への展望2010

    • Author(s)
      徳田岳
    • Organizer
      第12回建設・環境マネジメント講演会
    • Place of Presentation
      山口大学(山口県山口市)
    • Year and Date
      2010-02-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Similarities in midgut cellulolytic environment between a higher termite and a lower termite.2010

    • Author(s)
      Tokuda, G., 他4名
    • Organizer
      XVI International Congress of IUSSI
    • Place of Presentation
      Copenhagen, Denmark.
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] Similarities in midgut cellulolytic environment between a higher termite and a lower termite2010

    • Author(s)
      Tokuda, G.
    • Organizer
      XVI International Congress of IUSSI
    • Place of Presentation
      コペンハーゲン大学(デンマーク)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高等シロアリと下等シロアリ間における中腸セルロース消化システムの類似性2010

    • Author(s)
      徳田岳
    • Organizer
      (社)日本動物学会第81回大会
    • Place of Presentation
      東京大学(東京)
    • Related Report
      2010 Annual Research Report
  • [Presentation] バイオマス転換の前処理装置モデルとしてのシロアリ1)シロアリの消化機構の解明2009

    • Author(s)
      藤田愛, 他7名
    • Organizer
      第23回セルラーゼ研究会
    • Place of Presentation
      花王(株)霞ヶ浦研修所(茨城県美浦村)
    • Year and Date
      2009-06-19
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] バイオマス転換の前処理装置モデルとしてのシロアリ 1)シロアリの消化機構の解明2009

    • Author(s)
      藤田愛
    • Organizer
      第23回セルラーゼ研究会
    • Place of Presentation
      花王(株)霞ヶ浦研修所(茨城県稲敷郡美浦村)
    • Year and Date
      2009-06-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] An overview of the cellulolytic system in the wood-feeding termite2008

    • Author(s)
      Nasutitermes takasagoensis(Shiraki). Mie BioforumTokuda, G. & Watanabe, H
    • Organizer
      Nasutitermes takasagoensis(Shiraki). Mie Bioforum
    • Place of Presentation
      Mie, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] An overview of the cellulolytic system in the wood-feeding termite, Nasutitermes takasagoensis (Shiraki).2008

    • Author(s)
      Tokuda, G., 他1名
    • Organizer
      Mie Bioforum 2008
    • Place of Presentation
      Mie, Japan.
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] An overview of the cellulolytic system in the wood-feeding higher termite, Nasutitermes takasagoensis (Shiraki)2008

    • Author(s)
      Tokuda, G.
    • Organizer
      Mie Bioforum 2008
    • Place of Presentation
      三重県志摩市
    • Related Report
      2008 Annual Research Report
  • [Book] Evolution and function of endogenous termite cellulases.2011

    • Author(s)
      Lo, N., Tokuda, G., & Watanabe, H
    • Publisher
      Springer
    • Related Report
      2011 Final Research Report
  • [Book] Evolution and function of endogenous termite cellulases. In(Biology of Termites : a Modern Synthesis)(Bignell, D.E., Lo, N. & Roisin, Y., eds.)2011

    • Author(s)
      Lo, N., Tokuda, G., Watanabe, H.
    • Publisher
      Springer
    • Related Report
      2010 Self-evaluation Report
  • [Book] Biology of Termites : A Modern Synthesis2011

    • Author(s)
      Lo, N.
    • Publisher
      Springer
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.cc.u-ryukyu.ac.jp/~tokuda/

    • Related Report
      2011 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.cc.u-ryukyu.ac.jp/~tokuda/

    • Related Report
      2010 Self-evaluation Report
  • [Remarks]

    • URL

      http://www.cc.u-ryukyu.ac.jp/~tokuda/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.cc.u-ryukyu.ac.jp/~tokuda/

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www/cc.u-ryukyu.ac.jp/~comb/

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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