Analysis of structural elements controlling transglycosylation and its application
| Project/Area Number |
20380056
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
KIMURA Atsuo Hokkaido University, 大学院・農学研究院, 教授 (90186312)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OKUYAMA Masayuki 北海道大学, 大学院・農学研究院, 助教 (00344490)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2008: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
|
|---|
| Keywords | 糖転移 / 構造因子 / 分子解析 |
|---|
| Research Abstract |
Glycosylases are enzymes, which are currently utilized for the production of oligosaccharides. Interestingly, production-ability is dependent on the kinds of glycosylases, meaning that their structural elements contribute to the reaction specificity. Our research aims at elucidating structural elements of glycosylase-catalyzed transglycosylation. Our research achieved i) elucidation of structural elements contributing to the oligosaccharide production ; ii) establishment of the theoretical background of oligosaccharide production ; iii) finding the ways to regulate the oligosaccharide production.
|
Report
(4 results)
Research Products
(111 results)
-
-
-
-
-
-
-
-
[Journal Article] Optimal expression and characterization of a fusion enzyme having dextransucrase and dextranase activities2010
Author(s)
Ryu HJ, Jin X, Lee JH, Woo HJ, Kim YM, Kim GJ, Seo ES, Kang HK, Kim J, Cho DL, Kimura A, Kim D
-
Journal Title
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Molecular Mechanism of novel enzymes degrading and forming α-1,6-glucosidic linkages, including megalosaccharide production.2011
Author(s)
Kim YM, Kanegae M, Hondoh H, Nishimura T, Saburi W, Sadahiro J, Lang W, Shimizu R, Nakai H, Kang HK, Okuyama M, Mori H, Kang MS, Fujimoto Z, Funane K, Kim D, Kimura A
Organizer
The 78th Annual Meeting and International Symposium in 2011 Annual Conference of Korean Society of Food Science and Technology.
Place of Presentation
Daegu Exhibition Convention Center (Daegu, Korea).
Year and Date
2011-06-09
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Book] Carbohydrate-active enzymes : structure, function, and applications(ed.by Park K-H)(Molecular mechanism of α-glucosidase.)2008
Author(s)
Okuyama M, Mori H, Hondoh H, Nakai H, Saburi W, Kang MS, Kim YM, Nishimoto M, Wongchawalit J, Yamamoto T, Son M, Lee JH, Mar SS, Fukuda K, Chiba S, Kimura A
Publisher
Woodhead Publishing Ltd. (Cambridge, UK)
Related Report
-
-
-
