Development of chemical biology based on allosamidin molecule
| Project/Area Number |
20380066
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SAKUDA Shohei The University of Tokyo, 大学院・農学生命科学研究科, 准教授 (80192087)
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| Co-Investigator(Renkei-kenkyūsha) |
INOUE Hiromasa 鹿児島大学, 大学院・医歯学総合研究科, 教授 (30264039)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2008: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 生物活性物質 / キチナーゼ / 喘息 / キチナーゼ様タンパク質 / アロサミジン / 放線菌 / 二次代謝 / 生合成 / キチナーゼ阻害物質 / Ym1 / デメチルアロサミジン / キチナーゼ阻害剤 / キチン / 二次代謝産物 |
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| Research Abstract |
Allosamidin, a chitinase inhibitor, shows anti-asthmatic and chitinase production promoting activities toward mammals and Streptomyces bacteria, respectively. Allosamidin photo-affinity probe was used to identify a chitinase-like protein as a key allosamidin-binding molecule for anti-asthmatic activity of allosamidin. Expression systems to obtain recombinant candidate proteins for allosamidin's target molecule including the chitinase-like protein were constructed. Gene cluster responsible for allosamidin biosynthesis was obtained, which is useful for investigating the role of allosamidin in environment.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article]2011
Author(s)
Yosuke Sato, Shigeo Suzuki, Seiko Muraoka, Naoya Kikuchi, Naotaka Noda, Takafumi Matsumoto, Hiromasa Inoue, Hiromichi Nagasawa, Shohei Sakuda
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Journal Title
Bioorg.Med.Chem. 19
Pages: 3054-3059
Related Report
Peer Reviewed
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[Journal Article] Transglycosylation reaction catalyzed by a class V chitinase from cycad2010
Author(s)
Toki Taira, Maho Fujiwara, Nicole Dennhart, Hiroko Hayashi, Shoko Onaga, Takayuki Ohnuma, Thomas Letzel, Shohei Sakuda, Tamo Fukamizo
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Journal Title
Cycas revolute : A study involving site-directed mutagenesis, HPLC, and real-time ESI-MS. Biochimica et Biophysica Acta 1804
Pages: 668-675
Related Report
Peer Reviewed
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[Journal Article] Demethylallosamidin, a chitinase inhibitor, suppresses airway inflammation and hyperresponsiveness.2009
Author(s)
Takafumi Matsumoto, Hiromasa Inoue, Yosuke Sato, Yoshihiro Kita, Takako Nakano, Naotaka Noda, Miyuki Eguchi-Tsuda, Atsushi Moriwaki, Keiko Kan-o, Koichiro Matsumoto, Takao Shimizu, Hiromichi Nagasawa, Shohei Sakuda, Yoichi Nakanishi
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Journal Title
Biochem.Biophys.Res.Commun. 390
Pages: 103-108
Related Report
Peer Reviewed
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