Evaluation of the insect parasitic nematode, Sphaerularia vespae, as a biological control agent for hornets

• Project/Area Number: 20380097
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Forest science
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: KOSAKA Hajime Forestry and Forest Products Research Institute, 九州支所, グループ長 (20343791)
• Co-Investigator(Kenkyū-buntansha): SAYAMA Katsuhiko 独立行政法人森林総合研究所, 北海道支所, 主任研究員 (70353711) ; MAKINO Shun'ichi 独立行政法人森林総合研究所, 森林昆虫研究領域, 領域長 (20353849) ; KANZAKI Natsumi 独立行政法人森林総合研究所, 森林微生物研究領域, 主任研究員 (70435585)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000); Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2008: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
• Keywords: 森林生物 / 昆虫寄生線虫 / 生態解明 / 野外調査 / スズメバチ / 不妊化 / 寄生線虫 / 宿主範囲 / 地理的分布 / 感染場所 / 影響評価 / オオスズメバチ / 熊本 / 西表 / キイロスズメバチ / 寄生率

Research Abstract

Recently, we found an insect-parasitic nematode from the queen of common hornet, Vespa simillima, from Hokkaido and described it as a new species, Sphaerularia vespae. We also confirmed S. vespae sterilizes the queen hornets. In this study, we examined the host range, geographical distribution and life history of the nematode. Then we clarified that S. vespae parasitizes V.mandarinia and V.dybowskii in addition to V.simillima among seven species of Japanese hornets, (Vespa spp.) ; inhabits three Japanese main islands (Hokkaido, Honshu, and Kyushu) ; and infests gynes (potential queens of next generation) in their hibernation site. In consideration of its hosts including two most dangerous species, V.mandarinia and V.simillima, its wide geographical distribution in Japan and its infection route, we concluded that S. vespae is a promising candidate for the biological control agent of hornets.

Research Products

• [Journal Article] First record of occurrence of Xenos moutoni (Strepsiptera ; Stylopidae), an important parasite of hornets (Hymenoptera : Vespidae: Vespa), in Korea (2011)
  • Author(s): Makino S.、Kawashima M.、、Kosaka H.
  • Journal Title: Journal of Asia-Pacific Entomology Vol.14 Pages: 137-139
  • Peer Reviewed
• [Journal Article] Poikilolaimus carsiops n. sp. (Rhabditida : Rhabditidae) associated with Neotermes koshunensis (Kalotermitidae) in Kenting National Park, Taiwan (2011)
  • Author(s): Kanzaki N., Li H.F., Lan Y.C., Kosaka, H., Giblin-Davis R.
  • Journal Title: Nematology Vol.13 Pages: 155-164
  • Peer Reviewed
• [Journal Article] Poikilolaimus carsiops n.sp.(Rhabditida : Rhabditidae) associated with Neotermes koshunensis (Kalotermitidae) in Kenting National Park, Taiwan (2011)
  • Author(s): Kanzaki, N., Li, H.F., Lan, Y.C., Kosaka, H., Giblin-Davis, R.
  • Journal Title: Nematology Volume: 13 Pages: 155-164
  • Peer Reviewed
• [Journal Article] First record of occurrence of Xenos moutoni (Strepsiptera ; Stylopidae), an important parasite of hornets (Hymenoptera : Vespidae : Vespa), in Korea (2011)
  • Author(s): Makino, S., Kawashima, M., Kosaka, H
  • Journal Title: Journal of Asia-Pacific Entomology Volume: 14 Pages: 137-139
  • Peer Reviewed
• [Journal Article] Pseudaphelenchus vindai n. sp. (Tylenchomorpha : Aphelenchoididae) associated with termites (Termitidae) in Barro Colorado Island, Panama (2010)
  • Author(s): Kanzaki N., Giblin-Davis R.M., Herre E.A., Scheffrahn R.H., Center B.J.
  • Journal Title: Nematology Vol.12 Pages: 905-914
  • Peer Reviewed
• [Journal Article] Ditylenchus halictus n. sp. (Nematoda : Anguinidae), an associate of the sweat bee, Halictus sexcinctus (Halictidae), from Germany (2010)
  • Author(s): Giblin-Davis R.M., Erteld C., Kanzaki N., Ye W., Zeng Y., Center B.J.
  • Journal Title: Nematology Vol.12 Pages: 847-866
  • Peer Reviewed
• [Journal Article] スズメバチタマセンチュウ-あらたに発見されたスズメバチの天敵- (2010)
  • Author(s): 小坂肇
  • Journal Title: 山林 1516号 Pages: 64-67
• [Journal Article] Acrostichus megaloptae n. sp. (Nematoda : Diplogastridae), a phoretic associate of Megalopta spp. (Hymenoptera : Halictidae) in Central America (2010)
  • Author(s): Kanzaki N., Giblin-Davis R.M., Wcislo W.T., Zeng Y., Ye W., Cente, B.J., Esquivel A., Thomas W.K.
  • Journal Title: Nematology Vol.12 Pages: 453-468
  • Peer Reviewed
• [Journal Article] 北海道におけるツヤクロスズメバチの越冬記録 (2010)
  • Author(s): 佐山勝彦、小坂肇
  • Journal Title: つねきばち 17号 Pages: 29-30
• [Journal Article] Smaller nests of the hornet Vespa analis (Hymenoptera, Vespidae) are more severely affected by the strepsipteran parasite Xenos moutoni (Strepsiptera, Stylopidae) than are larger nests (2010)
  • Author(s): Makino S., Yamaura Y., Yamauchi H.
  • Journal Title: Insectes Sociaux Vol.57 Pages: 83-90
  • Peer Reviewed
• [Journal Article] Smaller nests of the hornet Vespa analis (Hymenoptera, Vespidae) are more severely affected by the strepsipteran parasite Xenos moutoni (Strepsiptera, Stylopidae) than are larger nests (2010)
  • Author(s): Makino, S., Yamaura, Y., Yamauchi, H.
  • Journal Title: Insectes Sociaux Volume: 57 Pages: 83-90
  • Peer Reviewed
• [Journal Article] Acrostichus megaloptae n.sp.(Nematoda : Diplogastridae), a phoretic as sociate of Megalopta spp.(Hymenoptera : Halictidae) in Central America. (2010)
  • Author(s): Kanzaki, N., Giblin-Davis, R.M., Wcislo, W.T., Zeng, Y., Ye, W., Center, B.J., Esquivel, A., Thomas, W.K.
  • Journal Title: Nematology Volume: 12 Pages: 453-468
  • Peer Reviewed
• [Journal Article] Ditylenchus halictus n.sp.(Nematoda : Anguinidae), an associate of the sweat bee, Halictus sexcinctus (Halictidae), from Germany. (2010)
  • Author(s): Giblin-Davis, R.M., Erteld, C., Kanzaki, N., Ye, W., Zeng, Y., Center, B.J.
  • Journal Title: Nematology Volume: 12 Pages: 847-866
  • Peer Reviewed
• [Journal Article] Pseudaphelenchus vindai n.sp.(Tylenchomorpha : Aphelenchoididae) associated with termites (Termitidae) in Barro Colorado Island, Panama. (2010)
  • Author(s): Kanzaki, N., Giblin-Davis, R.M., Herre, E.A., Scheffrahn, R.H., Center, B.J.
  • Journal Title: Nematology Volume: 12 Pages: 905-914
  • Peer Reviewed
• [Journal Article] 北海道におけるツヤクロスズメバチの越冬記録 (2010)
  • Author(s): 佐山勝彦、小坂肇
  • Journal Title: つねきばち Volume: 17 Pages: 29-30
• [Journal Article] スズメバチタマセンチュウ-あらたに発見されたスズメバチの天敵- (2010)
  • Author(s): 小坂肇
  • Journal Title: 山林 Volume: 1516 Pages: 64-67
• [Journal Article] スズメバチタマセンチュウ:新たに発見されたスズメバチ女王を不妊にする寄生虫 (2009)
  • Author(s): 小坂肇、佐山勝彦、神崎菜摘、高橋純一、牧野俊一
  • Journal Title: 森林保護 313号 Pages: 1-3
• [Journal Article] スズメバチタマセンチュウ : 新たに発見されたスズメバチ女王を不妊にする寄生虫 (2009)
  • Author(s): 小坂肇, 佐山勝彦, 神崎菜摘, 高橋純一, 牧野俊一
  • Journal Title: 森林保護 313 Pages: 1-3
• [Presentation] スズメバチ女王を不妊にするスズメバチタマセンチュウについて (2010)
  • Author(s): 小坂肇
  • Organizer: 微生物をめぐる生物間相互作用に関する小集会
  • Place of Presentation: 鹿児島大学高隅演習林(鹿児島県垂水市)(招待講演)
  • Year and Date: 2010-12-19
• [Presentation] スズメバチ女王を不妊にするスズメバチタマセンチュウについて (2010)
  • Author(s): 小坂肇
  • Organizer: 微生物をめぐる生物間相互作用に関する小集会
  • Place of Presentation: 鹿児島大学高隈演習林(鹿児島県垂水市)(招待講演)
  • Year and Date: 2010-12-19
• [Presentation] 寄生線虫に操作されたキイロスズメバチ女王の行動 (2010)
  • Author(s): 佐山勝彦、小坂肇、牧野俊一
  • Organizer: 日本動物行動学会大会
  • Place of Presentation: 沖縄県男女共同参画センターてぃるる(沖縄県那覇市)
  • Year and Date: 2010-11-19
• [Presentation] キイロスズメバチ女王におけるスズメバチタマセンチュウ寄生率の年次変動 (2010)
  • Author(s): 佐山勝彦、小坂肇、牧野俊一
  • Organizer: 日本昆虫学会大会
  • Place of Presentation: 山形大学(山形県山形市)
  • Year and Date: 2010-09-19
• [Presentation] 北海道産セイヨウオオマルハナバチの寄生線虫 (2010)
  • Author(s): 小坂肇、神崎菜摘、佐山勝彦、牧野俊一、岡部貴美子
  • Organizer: 日本線虫学会大会
  • Place of Presentation: 北海道大学(北海道札幌市)
  • Year and Date: 2010-08-27
• [Presentation] Uterium of the insect parasitic nematode Sphaerularia vespae (Nematoda, Sphaerulariidae), TEM obserbations (2010)
  • Author(s): Yushin V., Kosaka H.
  • Organizer: International congress on Invertebrate Reproduction and Development in the Age of Genetic Modifications
  • Place of Presentation: ホテル・クリスタルプラハ(チェコ・プラハ)
  • Year and Date: 2010-08-17
• [Presentation] Uterium of the insect parasitic nematode Sphaerulari vespae (Nematoda, Sphaerulariidae), TEM obserbations (2010)
  • Author(s): Yushin, V., Kosaka, H.
  • Organizer: International congress on Invertebrate Reproduction and Development in the Age of Genetic Modifications
  • Place of Presentation: ホテル・クリスタルプラハ(チェコ・プラハ)
  • Year and Date: 2010-08-17
• [Presentation] Nematode-parasitized hornets visit overwintering sites in summer to release juvenile parasites for transmission (2010)
  • Author(s): 佐山勝彦, 小坂肇, 牧野俊一
  • Organizer: XVI Congress of the International Union for the Study of Social Insects
  • Place of Presentation: コペンハーゲン大学・ペーノム研究所(デンマーク・コペンハーゲン)
  • Year and Date: 2010-08-12
• [Presentation] Nematode-parasitized hornets visit overwintering sites in summer to release juvenile parasites for transmission (2010)
  • Author(s): Sayama, K., Kosaka, H., Makino, S.
  • Organizer: XVI Congresss of the International Union for the Study of Social Insects
  • Place of Presentation: パヌム研究所(デンマーク・コペンハーゲン)
  • Year and Date: 2010-08-12
• [Presentation] Relationship between nest size of the hornet Vespa analis and prevalence of its parasite Xenos moutoni (2010)
  • Author(s): Makino, S., Yamamura, Y., Yamauchi, H.
  • Organizer: XVI Congresss of the International Union for the Study of Social Insects
  • Place of Presentation: パヌム研究所(デンマーク・コペンハーゲン)
  • Year and Date: 2010-08-12
• [Presentation] 寄生線虫に操られるキイロスズメバチ女王 (2010)
  • Author(s): 佐山勝彦、小坂肇、牧野俊一
  • Organizer: 日本応用動物昆虫学会会大会
  • Place of Presentation: 千葉大学(千葉県千葉市)
  • Year and Date: 2010-03-28
• [Presentation] 寄生線虫に操られるキイロスズメバチ女王 (2010)
  • Author(s): 佐山勝彦、小坂肇、牧野俊一
  • Organizer: 日本応用動物昆虫学会会大会
  • Place of Presentation: 千葉大学
  • Year and Date: 2010-03-28
• [Presentation] Termite associated nematodes : their association pattern and evolutional relationship (2010)
  • Author(s): Kanzaki N.
  • Organizer: Symposium and Workshop of Termite Biology and Control in Taiwan
  • Place of Presentation: 台湾大学(台湾・台北)(招待講演)
• [Presentation] Sphaerularia sp. from the introduced bumblebee, Bombus terrestris, naturalized in Hokkaido, Japan (2010)
  • Author(s): Kosaka H., Kanzaki N., Sayama, K., Makino S., Okabe K.
  • Organizer: International Symposium of the European Society of Nematologists
  • Place of Presentation: ボクー大学(オーストリア・ウィーン)
• [Presentation] Termite-associated nematodes in Kenting National Park, Taiwan and Miyako and Hateruma islands, Okinawa, Japan. (2010)
  • Author(s): Kanzaki, N., Li, H.-F., Lan, T.-C., Kosaka, H., Giblin-Davis, R.M., Akiba, M., Sahashi, N., Ota, Y.
  • Organizer: XXIII IUFRO World Congress
  • Place of Presentation: コエックス・センター(韓国・ソウル)
• [Presentation] Sphaerularia sp.from the introduced bumblebee, Bombus terrestris, naturalized in Hokkaido, Japan. (2010)
  • Author(s): Kosaka, H., Kanzaki, N., Sayama, K., Makino, S., Okabe, K.
  • Organizer: The European Society of Nematologists held its 30th International Symposium
  • Place of Presentation: ボクー大学(オーストリア・ウィーン)
• [Presentation] Termite associated nematodes : their association pattern and evolutional relationship. (2010)
  • Author(s): Kanzaki, N.
  • Organizer: Symposium and Workshop of Termite Biology and Control in Taiwan
  • Place of Presentation: 台湾大学(台湾・台北)(招待講演)
• [Presentation] 誘引餌の違いによるスズメバチ捕獲効率の検討 (2009)
  • Author(s): 小坂肇、佐山勝彦、牧野俊一
  • Organizer: 日本森林学会九州支部大会
  • Place of Presentation: 九州大学
  • Year and Date: 2009-10-17
• [Presentation] キイロスズメバチ女王に寄生したタマセンチュウは寄主の越冬行動を利用する (2009)
  • Author(s): 佐山勝彦、小坂肇、牧野俊一
  • Organizer: 日本昆虫学会大会
  • Place of Presentation: 三重大学
  • Year and Date: 2009-10-11
• [Presentation] 韓国におけるスズメバチネジレバネの記録 (2009)
  • Author(s): 牧野俊一、川島充博、小坂肇
  • Organizer: 日本昆虫学会大会
  • Place of Presentation: 三重大学
  • Year and Date: 2009-10-10
• [Presentation] 西表島におけるスズメバチ捕獲事例 (2009)
  • Author(s): 小坂肇、加藤一隆、佐山勝彦、牧野俊一
  • Organizer: 亜熱帯森林・林業研究会
  • Place of Presentation: 八汐荘大ホール(沖縄・那覇)
  • Year and Date: 2009-09-04
• [Presentation] Sphaerularia nematodes from native hornets and bumblebees in Hokkaido, Japan (2009)
  • Author(s): Kosaka H., Sayama K., Kanzaki N., Makino S.
  • Organizer: Nematodes in Tropical Ecosystem
  • Place of Presentation: アーミーホテル(ベトナム・ハノイ)
  • Year and Date: 2009-08-17
• [Presentation] Sphaerularia nematodes from native hornets and bumblebees in Hokkaido, Japan (2009)
  • Author(s): kosaka, H., Sayama, K., Kanzaki, N., Makino, S.
  • Organizer: Nematodes in Tropical Ecosystem
  • Place of Presentation: ベトナム・ハノイ
  • Year and Date: 2009-08-17
• [Presentation] スズメバチタマセンチュウに寄生されたキイロスズメバチ女王の行動 (2008)
  • Author(s): 佐山勝彦, 小坂肇, 牧野俊一
  • Organizer: 第68回日本昆虫学会大会
  • Place of Presentation: 香川大学
  • Year and Date: 2008-09-14
• [Presentation] The life cycle of Sphaerularia vespae, a novel parasite of hornets (2008)
  • Author(s): Kosaka H., Sayama K., Kanzaki N., Takahashi J., Makino S.
  • Organizer: 5th International Congress of Nematology
  • Place of Presentation: ブリスベン会議展示センター(オーストラリア・ブリスベン)
  • Year and Date: 2008-07-15
• [Presentation] The life cycle of Sphaerularia vespae, a novel parasite of hornets (2008)
  • Author(s): Kosaka, H., Sayama, K., Kanzaki, N., Takahashi, J., Makino, S.
  • Organizer: 5th International Congress of Nematology
  • Place of Presentation: オーストラリア・ブリスベン
  • Year and Date: 2008-07-15
• [Remarks] ホームページ等
• [Remarks] 研究代表者の小坂が、平成22年8月21日に一般人を対象とした森林総合研究所・九州支所第35回森のセミナーで「スズメバチに刺されないために」と題する講演をし、内容が地方テレビ局で放映された。
• [Remarks]
  • URL: http://www.ffpri-kys.affrc.go.jp/
• [Remarks]
  • URL: http://www.ffpri-kys.affrc.go.jp/kysmr/main-mr.htm