Role of heterocellular gap-junctional communication between cardiomyocytes and myofibroblasts in genesis of ventricular tachy arrhytymias
| Project/Area Number |
20390115
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
TAKAMATSU Tetsuro Kyoto Prefectural University of Medicine, 医学研究科, 教授 (40154900)
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| Co-Investigator(Renkei-kenkyūsha) |
TANAKA Hideo 京都府立医科大学, 大学院・医学研究科, 准教授 (60236619)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2008: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Keywords | 不整脈 / 心筋梗塞 / 非心筋細胞 / ギャップ結合 / 急性心筋梗塞 / 致死的心室性不整脈 / 心筋細胞 / 筋線維芽細胞 / 興奮伝播 / スパイラル波 |
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| Research Abstract |
This study addressed the possibility that myofibroblasts (MF), produced during healing phase of myocardial infarct, play important role(s) in fatal ventricular tachyarrhythmias. In scrape loading of the left ventricular wall, neurobiotin loaded into the cardiomyocytes (CM) propagated to the surrounding MF via Cx43. CM culture of neonate revealed slower conduction of the CM tissue co-cultured with MF in the opposite side of the filter membrane cup. Arrhythmogenic depolzizations were observed in CM layer when MFs were cultured in filter-membrane on the opposite side. Taken together, MF plays important role in arrhythmias during MI.
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Report
(4 results)
Research Products
(38 results)
