A large animal experimental study to establish a clinical applicable method for lung transplantation utilizing non-heart beating donors

• Project/Area Number: 20390367
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Kyoto University
• Principal Investigator: DATE Hiroshi Kyoto University, 医学研究科, 教授 (60252962)
• Co-Investigator(Kenkyū-buntansha): KOIKE Kaoru 京都大学, 大学院・医学研究科, 教授 (10267164) ; BANDO Toru 京都大学, 大学院・医学研究科, 准教授 (20293954) ; TSUYOSHI Shoji 京都大学, 大学院・医学研究科, 助教 (80402840) ; CHEN Fengshi 京都大学, 大学院・医学研究科, 助教 (00452334) ; FUJINAGA Takuji 京都大学, 大学院・医学研究科, 助教 (00444456) ; OKAMOTO Toshihiro 京都大学, 大学院・医学研究科, COEリサーチアシスタント (90467465) ; SANO Yoshifumi 岡山大学, 医学部歯学部附属病院, 講師 (60322228) ; OTO Takahiro 岡山大学, 医学部歯学部附属病院, 助教 (40452578) ; YAMANE Masaomi 岡山大学, 医学部歯学部附属病院, 助教 (20432643)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2008: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
• Keywords: 心停止ドナー / 肺移植 / Ex-vivo evaluation / 温虚血 / 再灌流 / ex-vivo evaluation

Research Abstract

The purpose of this study was to establish a clinically applicable method for lung transplantation utilizing non-heart-beating donors under the collaboration between Kyoto University and Okayama University groups. Using a large animal ex-vivo perfusion (EVLP) model, both groups performed the experimental studies. At Kyoto University, we demonstrated the usefulness of ET-Kyoto solution as perfusion solution of EVLP and the effect of EVLP in repairing edematous lung graft. At Okayama University, the effect of removing cytokines (TNF α and IL-8) by absorbable membrane was investigated. We suggested that factors other than these cytokines might play a rule for the graft injury during EVLP.

Research Products

• [Journal Article] Effect of donor pre-mortem hypoxia and hypotension on graft function and start of warm ischemia in donation after cardiac death lung transplantation. (2011)
  • Author(s): Miyoshi K, Oto T, Otani T, Tanaka S, Harada M, Kakishita T, Hori S, Waki N, Yamane M, Miyoshi S.
  • Journal Title: J Heart Lung Transplant 30(4) Pages: 445-451
  • NAID: 130004505725
• [Journal Article] Comparison of extracellular-type-kyoto solution and perfadex as a preservation solution in a pig ex vivo lung perfusion model : impact of potassium level. (2011)
  • Author(s): Okamoto T, Chen F, Zhang J, Choi H, Yamada T, Morikawa H, Nakayama E, Bando T, Date H.
  • Journal Title: Transplant Proc 43(5) Pages: 1525-1528
  • NAID: 120003238948
• [Journal Article] Early effects of the ex vivo evaluation system on graft function after swine lung transplantation. (2011)
  • Author(s): Otani S, Oto T, Kakishita T, Miyoshi K, Hori S, Yamane M, Toyooka S, Miyoshi S.
  • Journal Title: Eur J Cardiothorac Surg Epub ahead of print
• [Journal Article] Establishment of an ex vivo lung perfusion model using non-heart-beating large pigs. (2010)
  • Author(s): Okamoto T, Chen F, Zhang J, Yamada T, Nakayama E, Morikawa H, Bando T, Date H.
  • Journal Title: Transplant Proc 42(5) Pages: 1598-1601
• [Journal Article] Suppression of inflammatory cytokines during ex vivo lung perfusion with an absorbent membrane. (2010)
  • Author(s): Kakishita T, Oto T, Hori S, Miyoshi K, Otani S, Yamamoto S, Waki N, Yoshida O, Okazaki M, Yamane M, Toyooka S, Sano Y, Miyoshi S.
  • Journal Title: Ann Thorac Surg 89(6) Pages: 1773-1779
• [Journal Article] Establishment of an ex vivo lung perfusion model using non-heart-beating large pigs (2010)
  • Author(s): Okamoto T, Date H(8番目)
  • Journal Title: Transplant Proc Volume: 42(5) Pages: 1598-1601
  • Peer Reviewed
• [Journal Article] Post transplant lymphoproliferative disorder following cytomegalovirus reactivation in a lung recipient (2010)
  • Author(s): Aoyama A, Date H(9番目)
  • Journal Title: Gen Thorac Cardiovasc Surg Volume: 58(5) Pages: 251-254
  • NAID: 10026263541
  • Peer Reviewed
• [Journal Article] Living-donor single-lobe lung transplant in a 6-year-old girl after 7-month mechanical ventilator support (2010)
  • Author(s): Shoji T, Date H(4番目)
  • Journal Title: J Thorac Cardiovasc Surg Volume: 139(5)
  • NAID: 120002511335
  • Peer Reviewed
• [Journal Article] Post-ischemic infusion of atrial natriuretic peptide attenuates warm ischemia-reperfusion injury in rat lung (2009)
  • Author(s): Aoyama A, Date H., et all
  • Journal Title: J Heart Lung Transplant 28(6) Pages: 628-34
  • Peer Reviewed
• [Journal Article] Lung and heart-lung transplantation (2009)
  • Author(s): Date H, Sweet SC.
  • Journal Title: Cardiolo Young 19Suppl 1 Pages: 45-8
  • Peer Reviewed
• [Journal Article] Diagnostic strategies for mediastinal tumors and cysts (2009)
  • Author(s): Date H
  • Journal Title: Thorac Surg Clin 19(1) Pages: 29-35
  • Peer Reviewed
• [Journal Article] Nebulized phosphodiesterase III inhibitor during warm ischemia attenuates pulmonary ischemia-reperfusion injury (2009)
  • Author(s): Zhang J, Chen F, Zhao X, Aoyama A, Okamoto T, Fujinaga T, Shoji T, Sakai H, Cui Y, Bando T, Date H
  • Journal Title: J Heart Lung Transplant 28 Pages: 79-84
  • Peer Reviewed
• [Journal Article] Postischemic infusion of atrial natriuretic peptide attenuates warm ischemia-reperfusion injury in rat lungs (2009)
  • Author(s): Aoyama A, Chen F, Fujinaga T, Sato A, Tsuruyatma T, Zhang J, Shoji T, Sakai H, Nakamura T. Date H. Wada H. Bando T
  • Journal Title: J Heart Lung Transplant (印刷中)
  • Peer Reviewed
• [Journal Article] Successful subzero nonfreezing preservation of rat lungs at ミ 2C utilizing a new supercooling technology (2008)
  • Author(s): Okamoto T, Nakamura T, Zhang J, Aoyama A, Chen F, Fujinaga T, Shoji T, Hamakawa H, Sakni H. Manabe T. Wada H. Date H Bando T
  • Journal Title: J Heart Lung Transplant 27 Pages: 1150-1157
  • Peer Reviewed
• [Presentation] Reconditioning of lungs from non-heart-beating donors with normothermic ex-vivo lung perfusion. (2011)
  • Author(s): Nakajima D, Yamada T, Chen F, Sakamoto J, Ohsumi A, Fujinaga T, Shoji T, Sakai H, Bando T, Date H.
  • Organizer: 31^<st> Annual Meeting and Scientific Sessions of International Society for Heart Lung Transplantation Society
  • Place of Presentation: サンディエゴ
• [Presentation] Hypothermic ex-vivo perfusion prevents ischemia reperfusion injury in rat lungs from non-heart-beating donors. (2011)
  • Author(s): Nakajima D, Yamada T, Chen F, Sakamoto J, Ohsumi A, Fujinaga T, Shoji T, Sakai H, Bando T, Date H.
  • Organizer: 31^<st> Annual Meeting and Scientific Sessions of International Society for Heart Lung Transplantation Society
  • Place of Presentation: サンディエゴ
• [Presentation] Protective effect of ventilation before organ procurement on graft lungs donated after cardiac death in a canine lung transplantation model. (2011)
  • Author(s): Sakamoto J, Yamada T, Chen F, Nakajima D, Ohsumi A, Fujinaga T, Shoji T, Sakai H, Bando T, Date H.
  • Organizer: 31^<st> Annual Meeting and Scientific Sessions of International Society for Heart Lung Transplantation Society
  • Place of Presentation: サンディエゴ
• [Presentation] Reconditioning of lungs with pulmonary edema in ex-vivo lung perfusion circuit. (2011)
  • Author(s): Yamada T, Nakajima D, Sakamoto J, Chen F, Okamoto T, Ohsumi A, Fujinaga T, Shoji T, Sakai H, Bando T, Date H.
  • Organizer: 31^<st> Annual Meeting and Scientific Sessions of International Society for Heart Lung Transplantation Society
  • Place of Presentation: サンディエゴ
• [Presentation] Effects of ex-vivo lung evaluation system on post-transplant graft function in swine donation after cardiac death (DCD) lung trasnsplantation. (2010)
  • Author(s): Otani S, Oto T, Kakishita T, Harada M, Yamamoto S, Miyoshi K, Okazaki M, Waki N, Hori S, Sugimoto S, Soh J, Yamane M, Toyoka S, Miyoshi S.
  • Organizer: The 6^<th> Lung Transplant Conference
  • Place of Presentation: 日仙台
  • Year and Date: 2010-05-13
• [Presentation] 小児に対する生体肺移植の技術革新 (2010)
  • Author(s): 伊達洋至
  • Organizer: 第110回躰外科学会学術集会
  • Place of Presentation: 名古屋(招待講演)
  • Year and Date: 2010-04-08
• [Presentation] Does the ex-vivo lung evaluation system affect post-transplant graft dysfunction in swine donation after cardiac death (DCD) lung transplantation? (2010)
  • Author(s): Otani S, Oto T, Kakishita T, Harada M, Yamamoto S, Miyoshi K, Okazaki M, Waki N, Hori S, Sugimoto S, Soh J, Yamane M, Toyoka S, Miyoshi S.
  • Organizer: 30^<th> Annual Meeting and Scientific Sessions of International Society for Heart Lung Transplantation Society
• [Presentation] 映像による私の手術手技 (2009)
  • Author(s): 伊達洋至
  • Organizer: 第109回日本外科学会学術集会
  • Place of Presentation: 福岡
• [Presentation] Living-donor lobar lung transplantation for pulmonary hypertension (2008)
  • Author(s): 伊達洋至
  • Organizer: The 2nd Oriental Congress of Cardiology
  • Place of Presentation: 上海(中華人民共和国)
  • Year and Date: 2008-05-31
• [Presentation] 肺Ex vivo回路を用いたドナー肺保護と機能評価の研究 (2008)
  • Author(s): 岡本俊宏、陳豊史、藤永卓司、張吉天、青山晃博、趙向東、高橋鮎子、里田直樹、庄司剛、阪井宏彰、板東徹、伊達洋至
  • Organizer: 第44回日本移植学会総会
  • Place of Presentation: 日大阪
• [Book] 小児肺移植の現状と問題点 (2009)
  • Author(s): 伊達洋至
  • Total Pages: 4
  • Publisher: 日本胸部臨床
• [Remarks] ホームページ等
  • URL: http://www.thoracic-kyoto-u.gr.jp/
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