The tumorigenicity and transmissible genetic risk in mice under the Chernobyl simulation

• Project/Area Number: 20510052
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Osaka University
• Principal Investigator: NAKAJIMA Hiroo Osaka University, 大学院・医学系研究科, 助教 (20237275)
• Co-Investigator(Kenkyū-buntansha): SAITO Tadashi 大阪大学, ラジオアイソトープ総合センター, 教授 (50153812) ; HONGYO Tadashi 大阪大学, 大学院・医学系研究科, 准教授 (90271569)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 生物影響 / チェルノブイリ原発事故 / 低線量・低線量率放射線被曝 / 遺伝毒性 / 発がん性 / セシウム-137 / シミュレーション実験 / リスクアセスメント / チェルノブイリ原発放射能汚染 / 生物濃縮 / 核種体内動態

Research Abstract

To simulate the radiocontamination in middle contaminated areas of Belarus (1997, 2005), mice were maintained for 8 months in the radioisotope facility with free access to drinking water containing ^<137>CsCl (0Bq/ml, 10Bq/ml and 100Bq/ml).
Mice were assessed the long term low dose rate and low dose internal and external radiation effect by the quantitative measurement of the contaminated radionuclides-induced DNA double-strand breaks by γ-H2AX foci in the organs. The micro nuclei test and the tumorigenicity of mouse lung were also examined under the same condition.
The number of γ-H2AX focus per hepatic cell was 1.4 (0Bq/ml), 9.5(10Bq/ml), 10.7(100Bq/ml), respectively. It was suggested that the genome damage caused internal low dose ^<137>Cs radiation were occurring chronically even in low dose contaminated area. But no significant difference was observed in the micro nuclei test. In addition, progress data (by 6 animals in each dose groups) is showing that the number of lung tumors per mouse induced by urethane under the same condition is 36.8(368/10) (0 Bq/ml), 44.1 (441/10) (10Bq/ml), 44.1 (441/10) (100Bq/ml), and the average of tumor diameter (mm) is 1.64, 1.67, 1.68, respectively.

Research Products

• [Journal Article] A rapid and easy method for the qualitative detection of intracellular deposition of inhaled nanoparticles (2011)
  • Author(s): Nakajima H.,, Ozaki K., Hongyo T., Narama I., Todo T.
  • Journal Title: Nanomedicine : Nanotechnology, Biology and Medicine (2011), doi:10.1016/j.nano. In press
• [Journal Article] Effects of fission neutrons on human thyr id tissues aintained in SCID mice (2010)
  • Author(s): Adachi S., Ryo H., Hong T., Nakajima H., Tsuboi-Kikuya R., Tokita Y., Matsuzuka F., Hiramatsu K., Fujikawa K., Itoh T., Nomura T.
  • Journal Title: Mutation Research 696 Pages: 107-113
• [Journal Article] No evidence of increased mutation rates at microsatellite loci in offspring of A-bomb survivors. (2010)
  • Author(s): Kodaira M., Roy H., Kamata N., Furukawa K., Takahashi N., Nakajima H., Nomura T., Nakamura N.
  • Journal Title: Radiation Research 173 Pages: 205-213
• [Journal Article] Mossbauer Study on the Polar Ferrimagnet GaFeO3. (2010)
  • Author(s): Nakamura S., Morimoto S., Saito T., Tsunoda Y.
  • Journal Title: J.Phys. C200
• [Journal Article] 宇宙環境の人体影響評価 (2010)
  • Author(s): 野村大成、梁治子、足立成基、時田偉子、堀家なな緒、中島裕夫、本行忠志、藤川和男、伊藤哲夫、落合俊昌、行徳淳一郎、桂洋介
  • Journal Title: Evaluation of Human Risk in Space Environment、Space Utiliz Res. 26 Pages: 249-251
• [Journal Article] Effects of fission neutrons on human thyroid tissues maintained in SCID mice (2010)
  • Author(s): Adachi, S., Nakajima, H., et al.
  • Journal Title: Mutation Research Volume: 696 Pages: 107-113
  • Peer Reviewed
• [Journal Article] No evidence of increased mutation rates at microsatellite loci in offspring of A-bomb survivors. (2010)
  • Author(s): Kodaira, M., Nakajima H., et al.
  • Journal Title: Radiation Research Volume: 173 Pages: 2015-213
  • Peer Reviewed
• [Journal Article] Evaluation of Human Risk in Space Environment (2010)
  • Author(s): Nomura.T., Nakajima H., et al.
  • Journal Title: Space Utiliz Res. Volume: 26 Pages: 249-251
• [Journal Article] No evidence of increased mutation rates at microsatellite loci in offspring of A-bomb survivors (2010)
  • Author(s): 小平美江子, 中島裕夫, 他
  • Journal Title: Radiation Research 173 Pages: 205-213
  • Peer Reviewed
• [Journal Article] ヒト臓器・組織置換マウス等を用いた宇宙放射線の人体および継世代リスクの基礎評価、Risk of Cosmic Radiation on Human Organs and Tissues Maintained in SCID Mice and Transgenerational Effects (2009)
  • Author(s): 野村大成、梁治子、足立成基、中島裕夫,本行忠志、藤川和男、伊藤哲夫、松塚文夫
  • Journal Title: Space Utiliz Res. 25 Pages: 42-45
• [Journal Article] Terahertz Absorption Spectra of Original and Generic Ceftazidime. (2009)
  • Author(s): Kawase M., Saito T., Ogawa M., Uejima H., Hatsuda Y., Kawanishi S. Y.Hirotani, M.Myotoku, K.Ikeda, K.Takano, M.Hangyo, K.Yamamoto, M.Tani
  • Journal Title: Anal.Sci. 25 Pages: 1483-1485
  • NAID: 10026780722
• [Journal Article] ヒト臓器・組織置換マウス等を用いた宇宙放射線の人体および継世代リスクの基礎評価巳 (2009)
  • Author(s): 野村大成, 中島裕夫, 他
  • Journal Title: Space Utihz Res. 25 Pages: 42-45
  • Peer Reviewed
• [Journal Article] 原爆被爆者の子供におけるマイクロサテライト突然変異のスクリーニング (2008)
  • Author(s): 小平美江子, 梁治子, 高橋規郎, 鎌田直子, 古川恭治, 中島裕夫, 野村大成, 中村典
  • Journal Title: 長崎医学会雑誌83(特集号) Pages: 345-8
• [Journal Article] Differential radiation sensitivity to morphological, functional and molecular changes of human thyroid tissues and bone marrow cells maintained in SCID mice. (2008)
  • Author(s): Taisei Nomura, Tadashi Hongyo, Hiroo Nakajima, Li Ya Li, Mukh Syaifudin, Shigeki Adachi, Haruko Ryo, Rajamanickam Baskar, Kazuyasu Fukuda, Yoshihiro Oka, Haruo Sugiyama, Fumio Matsuzuka
  • Journal Title: Mutation Research 657 Pages: 68-76
• [Journal Article] チェルノブイリシミュレーション実験-放射能汚染地域における低線量放射線内部被曝による生物影響- (2008)
  • Author(s): 中島裕夫
  • Journal Title: 大阪大学RIセンターニュース No.21 Pages: 2-4
• [Journal Article] チェルノブイリ高度、中度放射能汚染地域における動植物体内の137Csの分布と含量の時間的推、Ecological decrease and biological concentration of radionuclides in plants and animals after Chernobyl catastrophe, Proceedings of the Eight Workshop on Environmental Radioactivity (TaichiMiura and Norikazu Kinoshita eds.) (2008)
  • Author(s): 中島裕夫、斎藤直、梁治子、野村大成
  • Journal Title: KEK Proceedings 2007-16 Pages: 113-118
• [Journal Article] Differential radiation sensitivity to morphological, functional and molecular changes of human thyroid tissues and bone marrow cells maintained in SCID mice. (2008)
  • Author(s): Taisei Nomura
  • Journal Title: Mutation Research 657 Pages: 68-76
  • Peer Reviewed
• [Journal Article] Ecological decrease and biological concentration of radionuclides in plants and animals after Chernobyl catastrophe (2008)
  • Author(s): Hiroo Nakajima
  • Journal Title: Proceedings of the Eight Workshop on Environmental Radioactivity 16 Pages: 113-118
  • Peer Reviewed
• [Presentation] scidマウスと野生型マウス系統におけるγ-H2AXフォーカス消長の比較 (2010)
  • Author(s): 中島裕夫, 他
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-21
• [Presentation] scidマウスと野生型マウス系統におけるγ-H2AXフォーカス消長の比較 (2010)
  • Author(s): 中島裕夫、菊谷理絵、野村大成、藤堂剛
  • Organizer: 日本放射線影響学会第53回大会要旨集、γ-H2AX foci formation in white blood cells of wild type and scid mouse strains after high or low dose rate γ-rays irradiation. 2010
  • Place of Presentation: 京都
• [Presentation] 宇宙環境の人体影響評価 (2010)
  • Author(s): 野村大成、梁治子、足立成基、大平充宣、本行忠志、中島裕夫、伊藤哲夫、藤川和男、落合俊昌、行徳淳一郎、桂洋介、時田偉子、堀家なな緒
  • Organizer: 宇宙利用シンポジウム(第26回)
  • Place of Presentation: 宇宙航空研究開発機構・相模原キャンパス(相模原)
• [Presentation] チェルノブイリ放射能汚染シミュレーションにおけるマウス腫瘍形成性とゲノムストレス (2009)
  • Author(s): 中島裕夫, 他
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-12
• [Presentation] Ecological decrease, biological concentration and genome stress of radionuclides in plants and animals after Chernobyl catastrophe (2009)
  • Author(s): 中島裕夫, 他
  • Organizer: International Conference on Environmental Mutagens
  • Place of Presentation: Firenze-Italy
  • Year and Date: 2009-08-24
• [Presentation] Ecological decrease, biological concentration and genome stress of radionuclides in plants and animals after Chernobyl catastrophe. (2009)
  • Author(s): H Nakajima, T Saito, KG Yeliseeva, H Ryo, T Hongyo, Y Yamaguchi, NA Kartel, EV Krupnova, V Trusova, AM Voitovich, VS Piskunov, TP Smirnova, EI Anisimova, V Afonin, T Todo, T Nomura
  • Organizer: 10th International Conference on Environmental Mutagens (ICEM), pp221
  • Place of Presentation: Firenze-Italy
• [Presentation] チェルノブイリ放射能汚染シミュレーションにおけるマウス腫瘍形成性とゲノムストレス (2009)
  • Author(s): 中島裕夫、本行忠志、斎藤直、藤堂剛
  • Organizer: 日本放射線影響学会第52回大会要旨集82 The tumorigenicity and genome stress in mice under the Chernobyl simulation. pp82.
  • Place of Presentation: 広島
• [Presentation] ヒト臓器・組織置換マウス等を用いた宇宙放射線の人体および継世代リスクの基礎評価 (2009)
  • Author(s): 野村大成、梁治子、足立成基、中島裕夫、本行忠志、藤川和男、伊藤哲夫、松塚文夫
  • Organizer: 宇宙利用シンポジウム
  • Place of Presentation: 宇宙航空研究開発機構・相模原キャンパス(相模原)
• [Presentation] チェルノブイリ放射能汚染地域に生息する生物の体内核種動態と遺伝子へのストレス (2008)
  • Author(s): 中島裕夫
  • Organizer: 日本環境変異原学会(第37回大会)
  • Place of Presentation: 沖縄コンベンションセンター(沖縄)
  • Year and Date: 2008-12-04
• [Presentation] Mice genome stress in the Chernobyl simulation experiment (2008)
  • Author(s): Hiroo Nakajima
  • Organizer: International Mammalian Genome Conference(IMGC 22^<nd>, 2008)
  • Place of Presentation: Inst. of Mol. Genet. Academy of Sci. of the Czech Republic(Prague)
  • Year and Date: 2008-11-05
• [Presentation] Mice genome stress in the Chernobyl simulation experiment. (2008)
  • Author(s): H.Nakajima, T.Saito, T.Hongyo, T.Todo
  • Organizer: 22nd International Mammalian Genome Conference. Institute of Molecular Genetics. Prague, pp129, 2008
  • Place of Presentation: Czech Republic
• [Presentation] SCIDマウスに維持されたヒト甲状腺組織への核分裂放射線の影響 (2008)
  • Author(s): 足立成基, 野村大成, 梁治子, 本行忠志, 中島裕夫, 猪原秀典, 藤川和男, 伊藤哲夫
  • Organizer: 日本放射線影響学会大会,講演要旨集pp.259
  • Place of Presentation: 小倉
• [Presentation] マイクロサテライトでの突然変異率に対する原爆放射線の遺伝的影響は検出されなかった (2008)
  • Author(s): 小平美江子, 梁治子, 高橋規郎, 鎌田直子, 古川恭治, 中島裕夫, 野村大成, 中村典
  • Organizer: 日本放射線影響学会大会、講演要旨集, pp.264
  • Place of Presentation: 小倉
• [Presentation] チェルノブイリ放射能汚染地域に棲息する生物の体内核種動態と遺伝子へのストレス (2008)
  • Author(s): 中島裕夫、梁治子、野村大成、斎藤直、本行忠志、藤堂剛
  • Organizer: 日本環境変異原学会(第37回大会)、International Symposium on Genotoxicity Assesment(併催)要旨集pp171. Genome Stress, Ecological Decrease and Biological Concentration of Radionuclides in Organisms after Chernobyl Catastrophe.
  • Place of Presentation: 沖縄コンベンションセンター(沖縄)