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Exploration for a Detox Pathway-Discovery and haracterization of New Genes in the Pathway

Research Project

Project/Area Number 20510069
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation/Chemicals
Research InstitutionChubu University

Principal Investigator

MIWA Johji  Chubu University, 応用生物学部, 教授 (80321686)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsトキシコロジー / 生体異物代謝 / Caenorhabditis elegans / 薬毒物代謝 / GST / WDリピートタンパク質 / xrep遺伝子 / ストレス / ホルミシス / ホルメティック回路 / 異物代謝 / 遺伝学 / 経路 / アクリルアミド
Research Abstract

We are constantly exposed to harmful substances from foods and environments. Besides, our body also produces such metabolites quite unkind to our own tissues, cells, and genetic materials as reactive oxygen species. We do have innate defense systems, however, against these assaults. One of them involves a detoxifying (detox) enzyme system, where harmful substances are first recognized, captured, transformed, and finally expelled outside the body. We explored this system using the model animal C. elegans and have found that an XREP-1/XREP-3 pair plays a vital role in the detox system. XREP-1 functions as a sensor to recognize harmful substances and XREP-3 as a processor to initiate the production of detox enzymes. This pair resembles the mammalian Keap1/Nfr2 pair, indicating that a similar system is working in the human body.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (39 results)

All 2011 2010 2009 2008 Other

All Journal Article (10 results) (of which Peer Reviewed: 5 results) Presentation (19 results) Book (4 results) Remarks (4 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] 薬即是毒-ホルメティック回路による生体防衛2011

    • Author(s)
      三輪錠司、長谷川浩一、毒即是薬
    • Journal Title

      生物の科学 1月号

      Pages: 98-104

    • Related Report
      2010 Final Research Report
  • [Journal Article] 毒即是薬 薬即是毒-ホルメティック回路による生体防衛2011

    • Author(s)
      三輪錠司
    • Journal Title

      生物の科学

      Volume: 1月号 Pages: 98-104

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 毒と薬は紙一重-ホルメティック食品-2010

    • Author(s)
      三輪錠司
    • Journal Title

      青淵 11月号

      Pages: 12-14

    • Related Report
      2010 Final Research Report
  • [Journal Article] Genetic and cellular characterization of Caenorhabditis elegans mutants abnormal in the regulation of many phase II enzymes2010

    • Author(s)
      Koichi Hasegawa、Johji Miwa
    • Journal Title

      PLoS ONE Vol.5

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Allyl isoth iocyanate that induces GST and UGT expression confers oxidative stress resistance on C.elegans, as demonstrated by nematode biosensor2010

    • Author(s)
      Koichi Hasegawa, Satsuki Miwa、Kaname Tsutsumiuchi、Johji Miwa
    • Journal Title

      PLoS ONE Vol.5

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic and cellular characterization of Caenorhabditis elegans mutants abnormal in the regulation of many phase II enzymes2010

    • Author(s)
      長谷川浩一
    • Journal Title

      PLoS ONE

      Volume: 5

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptome analysis of the xrep-1(RNAi)phenocopy in C.elegans2010

    • Author(s)
      長谷川浩一
    • Journal Title

      The Annual Report of Research Institute for Biological Function

      Volume: 10 Pages: 13-21

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 毒と薬は紙一重-解毒に役立つホルメティック食品のすすめ-2010

    • Author(s)
      三輪錠司
    • Journal Title

      青淵

      Volume: 11月号 Pages: 12-14

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Allyl isothiocyanate that induces GST and UGT expression confers oxidative stress resistance on C.elegans, as demonstrated by nematode biosensor2010

    • Author(s)
      長谷川浩一
    • Journal Title

      PLoS ONE 5

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Early embryogenesis and anterior-posterior axis formation in the white-tip nematode Aphelenchoides besseyi2009

    • Author(s)
      吉田幸平
    • Journal Title

      Journal of Nematology 41

      Pages: 17-22

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 線虫学がC.elegansを取り込む日を目指して2011

    • Author(s)
      三輪錠司
    • Organizer
      第55回日本応用動物昆虫学会大会
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Acrylamide confers aldicarb resistance on Caenorhabditis elegans via the XREP-1/SKN-1 system2010

    • Author(s)
      Koichi Hasegawa、Johji Miwa
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場(神戸市)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] Acrylamide confers aldicarb resistance on Caenorbabditis elegans via the XREP-1/SKN-1 system2010

    • Author(s)
      長谷川浩一
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場(神戸市)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生体防衛機構で働く新規遺伝子xrep-3の遺伝学的解析2010

    • Author(s)
      長谷川浩一
    • Organizer
      日本線虫学会第18回大会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2010-08-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Genetic characterization of mutants in xenobiotics response pathways2010

    • Author(s)
      Koichi Hasegawa、Johji Miwa
    • Organizer
      The 4^<th> Ease Asia C.elegans Meeting
    • Place of Presentation
      国立オリンピック記念青少年総合センター(東京都)
    • Year and Date
      2010-07-12
    • Related Report
      2010 Final Research Report
  • [Presentation] Genetic characterization of mutants in xenobiotics response pathways2010

    • Author(s)
      長谷川浩一
    • Organizer
      The 4th East Asia C.elegans Meeting
    • Place of Presentation
      国立オリンピック記念青少年総合センター(東京都)
    • Year and Date
      2010-07-12
    • Related Report
      2010 Annual Research Report
  • [Presentation] Nematode biosensors to discover functional food ingredients : allylisothiocyanate that induces GST and UGT expression confers oxidative stress resistance on C.elegans2010

    • Author(s)
      長谷川浩一
    • Organizer
      The 4th East Asia C.elegans Meeting
    • Place of Presentation
      国立オリンピック記念青少年総合センター(東京都)
    • Year and Date
      2010-07-12
    • Related Report
      2010 Annual Research Report
  • [Presentation] マツノザイセンチュウドラフトゲノムの解読2010

    • Author(s)
      菊池泰生
    • Organizer
      第121回日本森林学会大会
    • Place of Presentation
      筑波大学(茨城県)
    • Year and Date
      2010-04-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] 線虫バイオセンサーを用いた機能性食品成分のスクリーニング2010

    • Author(s)
      長谷川浩一
    • Organizer
      第54回日本応用動物昆虫学会大会
    • Place of Presentation
      千葉大学(千葉市)
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Caenorhabditis elegans のWDR-23はGST発現を負に制御する2009

    • Author(s)
      長谷川浩一
    • Organizer
      日本線虫学会第17回大会
    • Place of Presentation
      崇城大学市民ホール(熊本市)
    • Year and Date
      2009-09-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] GST発現に関わる遺伝子の変異体解析2009

    • Author(s)
      近藤有希菜
    • Organizer
      日本線虫学会第17回大会
    • Place of Presentation
      崇城大学市民ホール(熊本市)
    • Year and Date
      2009-09-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] イネシンガレセンチュウ Aphelenchoides besseyi の初期胚発生と体軸決定について2009

    • Author(s)
      吉田幸平
    • Organizer
      日本線虫学会第17回大会
    • Place of Presentation
      崇城大学市民ホール(熊本市)
    • Year and Date
      2009-09-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] 線虫バイオセンサーを用いたアクリルアミド毒性緩和物質の探索2009

    • Author(s)
      木邑和広
    • Organizer
      日本線虫学会第17回大会
    • Place of Presentation
      崇城大学市民ホール(熊本市)
    • Year and Date
      2009-09-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] Pseudomonas aeruginosa による Caenorhabditis elegans 殺害に対するアクリルアミドの効果2009

    • Author(s)
      石川友洋
    • Organizer
      日本線虫学会第17回大会
    • Place of Presentation
      崇城大学市民ホール(熊本市)
    • Year and Date
      2009-09-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] モデル生物「線虫」を使った化学物質の安全性及び機能性評価2009

    • Author(s)
      長谷川浩一
    • Organizer
      中部大学生物機能開発研究所、健康食品科学寄附講座、植物バイオ研究センター共催セミナー
    • Place of Presentation
      中部大学(愛知県春日井市)
    • Year and Date
      2009-07-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] GST発現に異常をきたした変異体の解析2008

    • Author(s)
      山内春奈
    • Organizer
      日本線虫学会第16回大会
    • Place of Presentation
      筑波研修センター
    • Year and Date
      2008-09-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] 線虫バイオセンサーを用いたGST発現誘導物質のスクリーニングと生物に与える効能評価2008

    • Author(s)
      長谷川浩一
    • Organizer
      日本線虫学会第16回大会
    • Place of Presentation
      筑波研修センター
    • Year and Date
      2008-09-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] Nematode biosensors to identify toxicants and to discover their antidotes.2008

    • Author(s)
      長谷川浩一
    • Organizer
      5th International Congress of Nematology
    • Place of Presentation
      Brisbane, Australia
    • Year and Date
      2008-06-17
    • Related Report
      2008 Annual Research Report
  • [Presentation] Developmental biology of the white-tip nematode Aphelenchoides besseyi2008

    • Author(s)
      持地信雄
    • Organizer
      5th International Congress of Nematology
    • Place of Presentation
      Brisbane, Australia
    • Year and Date
      2008-06-17
    • Related Report
      2008 Annual Research Report
  • [Book] Pine Wilt Disease(Embryology and Cytology of Bursaphelenchus xylophilus)2008

    • Author(s)
      Koichi Hasegawa、Johji Miwa
    • Total Pages
      459
    • Publisher
      Springer
    • Related Report
      2010 Final Research Report
  • [Book] Pine Wilt Disease : A Worldwide Threat to Forest Ecosystem(Developmental Biology and Cytogenetics of Bursaphelenchus xylophilus)2008

    • Author(s)
      Koichi Hasegawa、Johji Miwa
    • Total Pages
      405
    • Publisher
      Springer
    • Related Report
      2010 Final Research Report
  • [Book] Pine Wilt Disease2008

    • Author(s)
      長谷川浩一
    • Publisher
      Springer Verlag
    • Related Report
      2008 Annual Research Report
  • [Book] Pine Wilt Disease : A Worldwide Threat to Forest Ecosystems2008

    • Author(s)
      長谷川浩一
    • Publisher
      Springer Verlag
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://stu.isc.chubu.ac.jp/bio/public/Environ_Bio/labo/miwa_lab/top.htm

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://stu.isc.chubu.ac.jp/bio/public/Environ_Bio/labo/miwa_lab/top.htm

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://stu.isc.chubu.ac.jp/bio/public/Environ_Bio/labo/miwa_lab/top.htm

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://stu.isc.chubu.ac.jp/bio/public/Environ_Bio/labo/miwa_lab/top.htm

    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] 線虫を用いたタンパク質の生産方法2008

    • Inventor(s)
      三輪錠司、長谷川浩一
    • Industrial Property Rights Holder
      中部大学
    • Industrial Property Number
      2008-304499
    • Filing Date
      2008-11-28
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 線形動物(通称、線虫類)を使用した有用タンパク質の生産2008

    • Inventor(s)
      三輪錠司, 長谷川浩
    • Industrial Property Rights Holder
      中部大学
    • Industrial Property Number
      2008-304499
    • Filing Date
      2008-11-28
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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