Study on functional antisense nucleic acids for ncRNA

• Project/Area Number: 20550147
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Gunma University
• Principal Investigator: OZAKI Hiroaki Gunma University, 大学院・工学研究科, 教授 (90211820)
• Co-Investigator(Renkei-kenkyūsha): INOUE Yusuke 群馬大学, 大学院・工学研究科, 准教授 (90304302)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 核酸 / アンチセンス / siRNA / アンセンス / mRNA / 修飾核酸 / ncRNA

Research Abstract

Functional oligonucleotides were prepared for new antisense molecule to regulate microRNA. Incorporation of arabinonucleosides or phenanthroline-polyamine derivatives in DNA did not contribute to enhancement of antisense activity. siRNA bearing modified nucleoside was more active in silencing the gene expression. These siRNA could regulate the microRNA.

Research Products

• [Journal Article] Synthesis of modified siRNA bearing C-5 polyamine-substituted pyrimidine nucleoside in their 3'-overhang regions and its RNAi activity (2011)
  • Author(s): Mohammad Mehedi Masud, Tomokazu Masuda, Yusuke Inoue, Masayasu Kuwahara, Hiroaki Sawai, Hiroaki Ozaki
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Vol.21, No.2 Pages: 715-717
  • Peer Reviewed
• [Journal Article] Synthesis of modified siRNA bearing C-5 polyamine-substituted Pyrimidine nucleoside in their 3'-overhang regions and its RNAi activity (2011)
  • Author(s): Mohammad Mehedi Masud
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 21 Pages: 715-717
  • Peer Reviewed
• [Journal Article] Synthesis and properties of oligodeoxyribonucleotides bearing a polyaminogroup at the 2' position via 2'-O-carbamoylmethyl and 2'-S-carbamoylmethyl groups (2009)
  • Author(s): Hiroaki Ozaki
  • Journal Title: Nucleosides, Nucleotides & Nucleic Acids 28 Pages: 943-952
  • Peer Reviewed
• [Presentation] 3'オーバーハング領域に修飾ヌクレオシドを導入したsiRNAの合成とそのRNAi活性の検討 (2010)
  • Author(s): 増田知和、Mohammad Mehedi Masud、井上裕介、桑原正靖、澤井宏明、尾崎広明
  • Organizer: 日本化学会関東支部群馬地区地域懇談会
  • Place of Presentation: 日本原子力研究開発機構(群馬県)
  • Year and Date: 2010-11-22
• [Presentation] 3'オーバーハング領域に修飾ヌクレオシドを導入したsiRNAの合成とそのRNAi活性の検討 (2010)
  • Author(s): 増田知和
  • Organizer: 日本化学会関東支部群馬地区地域懇談会
  • Place of Presentation: 高崎
  • Year and Date: 2010-11-22
• [Presentation] Synthesis of modified siRNA and its activity for gene regulation (2010)
  • Author(s): Mohammad Mehedi Masud, Tomokazu Masuda, Yusuke Inoue, Hiroaki Ozaki
  • Organizer: The 37th International symposium on Nucleic Acid Chemistry 2010
  • Place of Presentation: Hamagin Hall (Yokohama).
  • Year and Date: 2010-11-11
• [Presentation] Synthesis of modified siRNA and its activity for gene regulation (2010)
  • Author(s): 増田知和
  • Organizer: The 37th International symposium on Nucleic Acid Chemistry 2010
  • Place of Presentation: 横浜
  • Year and Date: 2010-11-10
• [Presentation] 1,10-フェナンスロリン-ポリアミン複合体の開発と核酸の切断活性の検討 (2010)
  • Author(s): 増田知和、Mohammad Mehedi Mohammad Mehedi、尾崎広明
  • Organizer: 日本化学会第4回関東支部大会
  • Place of Presentation: 筑波大学(筑波)
  • Year and Date: 2010-08-30
• [Presentation] 1,10-フェナンスロリン・ポリアミン複合体の開発と核酸の切断活性の検討 (2010)
  • Author(s): 増田知和
  • Organizer: 第4回関東支部大会
  • Place of Presentation: 筑波(筑波大学)
  • Year and Date: 2010-08-30
• [Presentation] 5位置換ピリミジンヌクレオシドを含む機能性核酸の合成とアンチセンス法への利用 (2009)
  • Author(s): 増田知和、尾崎広明、Mohammad Mehedi Mohammad Mehedi、〓原正靖、澤井宏明
  • Organizer: 日本化学会第3回関東支部大会
  • Place of Presentation: 早稲田大学(東京)
  • Year and Date: 2009-09-04
• [Presentation] 5位置換ピリミジンヌクレオシドを含む機能性核酸の合成とアンチセンス法への利用 (2009)
  • Author(s): 増田知和
  • Organizer: 日本化学会第3回関東支部大会
  • Place of Presentation: 東京(早稲田大学)
  • Year and Date: 2009-09-04