Molecular-biological studies on the function of cardiolipin in higher plants

• Project/Area Number: 20570031
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The University of Tokyo
• Principal Investigator: WADA HAJIME The University of Tokyo, 大学院・総合文化研究科, 教授 (60167202)
• Co-Investigator(Kenkyū-buntansha): MIZUSAWA Naoki 東京大学, 大学院・総合文化研究科, 助教 (80342856)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: カルジオリピン / 高等植物 / 脂質 / シロイヌナズナ / 生体膜 / ミトコンドリア / 膜脂質

Research Abstract

We studied physiological roles of CL with cls mutants of Arabidopsis thaliana, in which the CLS gene for CL synthase is disrupted by T-DNA insertion. The findings obtained in this study are as follows: (1) CLS expression can control the level of CL and CL plays important roles in growth of plants. (2) CLS gene is highly expressed in cells of tissues, such as the columella and guard cells, where the number of mitochondria is higher than that in cells of other tissues, suggesting importance of CLS in mitochondria-rich cells. (3) The shape of mitochondria in cls mutants is abnormal, namely, mitochondria in mutant cells are extremely larger than those in wild-type cells. These findings demonstrate that CL plays critical roles in maintenance of mitochondrial structure and plant development.

Research Products

• [Journal Article] T-DNA insertion in the CLS gene for cardiolipin synthase affects development of Arabidopsis thaliana. (2011)
  • Author(s): Katayama K, Wada H
  • Journal Title: Cytologia vol.76(in press)
  • NAID: 10030866335
  • Peer Reviewed
• [Journal Article] The role of lipids in photosystem II. (2011)
  • Author(s): Mizusawa N., Wada H.
  • Journal Title: Biochim.Biophys.Acta vol.1807(in press)
  • Peer Reviewed
• [Journal Article] Acyl lipid metabolism. (2010)
  • Author(s): Li-Beisson Y., Shorrosh B., Beisson F., Andersson M.X., Arondel V., Bates P.D., Baud S., Bird D., DeBono A., Durrett T.P., Franke R.B., Graham I.A., Katayama K., Kelly A.A., Larson T., Markham J.E., Miquel M., Molina I., Nishida I., Rowland O., Samuels L., Schmid K.M., Wada H., Welti R., Xu C., Zallot R., Ohlrogge J.
  • Journal Title: The Arabidopsis book. vol.8
  • Peer Reviewed
• [Journal Article] Phosphatidylglycerol depletion affects photosystem II activity in Synechococcus PCC 7942 cells (2010)
  • Author(s): Balazs Bogos
  • Journal Title: Photosynthesis Research 103 Pages: 19-30
  • Peer Reviewed
• [Journal Article] Remodeling of phosphatidylglycerol in Synechocystis PCC6803 (2010)
  • Author(s): Hajnalka Laczko-Dobos
  • Journal Title: Biochimica et Biophysica Acta 1801 Pages: 163-170
  • Peer Reviewed
• [Journal Article] Cellular function of the phospholipid cardiolipin. (2009)
  • Author(s): Katayama K., Wada H.
  • Journal Title: Plant Morphol. vol.21 Pages: 17-28
  • NAID: 130002152911
  • Peer Reviewed
• [Journal Article] Cellular function of the phospholipid cardiolipin. (2009)
  • Author(s): Kenta Katayama
  • Journal Title: Plant Morphology 21(掲載(発行)確定)
  • Peer Reviewed
• [Presentation] ホスファチジルグリセロールはシロイヌナズナの胚発生に必要である (2011)
  • Author(s): 和田元
  • Organizer: 日本植物生理学会
  • Place of Presentation: 仙台(東北大学)
  • Year and Date: 2011-03-20
• [Presentation] 植物におけるカルジオリピンの機能解析 (2011)
  • Author(s): 和田元
  • Organizer: 日本植物生理学会
  • Place of Presentation: 仙台(東北大学)
  • Year and Date: 2011-03-20
• [Presentation] Roles of anionic phospholipids, phosphatidylglycerol and cardiolipin, in photosynthetic organisms (2011)
  • Author(s): Hajime Wada
  • Organizer: Japanese-Finnish binational seminar
  • Place of Presentation: Okayama (Okayama Royal Hotel)
  • Year and Date: 2011-03-03
• [Presentation] Roles of anionic phospholipids, phosphatidylglycerol and cardiolipin, in photosynthetic organisms (2011)
  • Author(s): Hajime Wada
  • Organizer: Japan-Finnish Binational Seminar
  • Place of Presentation: 岡山ロイヤルホテル(岡山県岡山市)
  • Year and Date: 2011-03-03
• [Presentation] ホスファチジルグリセロールはシロイヌナズナの胚発生に必要である (2010)
  • Author(s): 和田元
  • Organizer: 日本植物脂質シンポジウム
  • Place of Presentation: 京都(京都大学)
  • Year and Date: 2010-11-27
• [Presentation] カルジオリピン合成酵素はカルジオリピン量を制御できる (2010)
  • Author(s): 和田元
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本(熊本大学)
  • Year and Date: 2010-03-21
• [Presentation] シロイヌナズナにおけるPG合成に関わるPGP合成酵素遺伝子の機能解析 (2010)
  • Author(s): 和田元
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本(熊本大学)
  • Year and Date: 2010-03-21
• [Presentation] カルジオリピン合成酵素はカルジオリピン量を制御できる (2010)
  • Author(s): 片山健太
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-20
• [Presentation] Roles of lipids in photosynthesis (2009)
  • Author(s): Hajime Wada
  • Organizer: The third Asian sy,posium on plant lipids
  • Place of Presentation: Yokohama (Yokohama Media and Communication Center)
  • Year and Date: 2009-11-27
• [Presentation] Roles of lipids in photosynthesis under stressed conditions (2008)
  • Author(s): Hajime Wada
  • Organizer: Japanese-Finnish binational seminar
  • Place of Presentation: Helsinki (Finland)
  • Year and Date: 2008-10-31
• [Presentation] 膜脂質研究に多細胞性をどう生かすかオルガネラ機能および発生におけるカルジオリピンの役割 (2008)
  • Author(s): 和田元
  • Organizer: 日本植物学会
  • Place of Presentation: 高知(高知大学)
  • Year and Date: 2008-09-25
• [Presentation] 膜脂質研究に多細胞性をどう生かすかオルガネラ機能および発生におけるカルジオリピンの役割 (2008)
  • Author(s): 片山健太
  • Organizer: 日本植物学会
  • Place of Presentation: 高知大学
  • Year and Date: 2008-09-25
• [Presentation] Cardiolipin synthase plays important roles in plant morphology (2008)
  • Author(s): Hajime Wada
  • Organizer: The 18th International Symposium on Plant Lipids
  • Place of Presentation: Bordeaux (France)
  • Year and Date: 2008-07-20
• [Book] Lipids in photosynthesis : Essential and regulatory functions. (2009)
  • Author(s): Wada H., Murata N.
  • Publisher: Springer
• [Book] Lipids in photosynthesis : Essential and regulatory functions. (2009)
  • Author(s): Wada H., Mizusawa N.
  • Publisher: Springer
• [Book] Lipids in Photosynthesis : Essential and regulatory functions. (2009)
  • Author(s): Sato N., Wada H.
  • Publisher: Springer
• [Book] Lipids in Photosynthesis : Essential and Regulatory Functions (2009)
  • Author(s): Hajime Wada
  • Total Pages: 473
  • Publisher: Springer
• [Book] Lipids in Photosynthesis:Essential and Regulatory Functions (2009)
  • Author(s): Hajime Wada
  • Publisher: Springer(掲載(発行)確定)
• [Remarks] ホームページ等
  • URL: http://hajimewada.c.u-tokyo.ac.jp/