A study of neuronal differentiation of sea cucumber by the comprehensive analysis of neuropeptides.
| Project/Area Number |
20570057
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Kyushu University |
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Principal Investigator |
YOSHIKUNI Michiyasu Kyushu University, 大学院・農学研究院, 教授 (50210662)
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| Co-Investigator(Renkei-kenkyūsha) |
OHNO Kaoru 自然科学研究機構, 基礎生物学研究所, 教 (10260035)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 比較内分泌 / 生体分子 / タンパク質 / 生理活性物質 / 棘皮動物 / プロテオーム / 神経 / 機能分化 / 蛋白質 / プロテオミクス / 卵成熟 |
|---|
| Research Abstract |
EST analysis and mass spectrometry revealed the low quality of neural tissues prepared by a conventional method. A complete dehydration could suppress the degradations of RNAs and peptides during microdissection. The ratio of a neural peptide mRNA and EF1a mRNA was improved over several dozen folds. A new peptide, Cubifirn, was identified as a potent neural factor to induce oocyte maturation and spawning.
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Report
(4 results)
Research Products
(54 results)
