Notochord-Neuron interaction during the chordate evolution.

• Project/Area Number: 20570214
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: TAKAHASHI Hiroki National Institute for Basic Biology, 形態形成研究部門, 助教 (40283585)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 脊索 / 神経 / 発生 / 進化 / ホヤ

Research Abstract

The midline organ notochord and its overlying dorsal neural tube are the most prominent features of the chordate body plan. Although molecular mechanisms involved in the dorsal patterning of the nervous system have been studied extensively in vertebrate embryos, none of the genes that are expressed specifically in notochord cells have been shown to function in this process. Here, we report a gene in the urochordate Ciona intestinalis encoding a fibrinogen-like protein that plays a pivotal role in the notochord-dependent dorsal positioning of neuronal cells. Therefore, it is highly likely that the interaction of the notochord-based fibrinogen-like protein and the neural tube-based Notch signaling is essential for the dorsal patterning of the nervous system or for the establishment of the chordate body plan.

Research Products

• [Journal Article] Ciona intestinalis Noto4 contains a phosphotyrosine interaction domain and is involved in the midline intercalation of notochord cells. (2011)
  • Author(s): Yamada S., Ueno N., Satoh N., Takahashi H.
  • Journal Title: Int.J.Dev.Biol. 55 Pages: 11-18
  • Peer Reviewed
• [Journal Article] CIPRO 2.5 : Ciona intestinalis Protein Database, a unique integrated repository of large-scale omics data, bioinformatic analyses, and curated annotation, with user rating and reviewing functionality. (2011)
  • Author(s): Endo T., Ueno K., Yonezawa K., Mineta K., Hotta K., Satou Y., Yamada L., Ogasawara M., Takahashi H., Nakajima A., Nakachi M., Nomura M., Yaguchi J., Sasakura Y., Yamasaki C., Sera M., Yoshizawa A., Imanishi T., Taniguchi H., Inaba K.
  • Journal Title: Nucleic Acids Res. 38
  • NAID: 120002834765
  • Peer Reviewed
• [Journal Article] CIPRO 2.5 : Ciona intestinalis Protein Database, a unique integrated repository of large-scale omics data, bioinformatic analyses, and curated annotation, with user rating and reviewing functionality. (2011)
  • Author(s): Endo, T., Ueno, K., Yonezawa, K., Mineta, K., Hotta, K., Satou, Y., Yamada, L., Ogasawara, M., Takahashi, H., Nakajima, A., Nakachi, M., Nomura, M., Yaguchi, J., Sasakura, Y., Yamasaki, C.Sera, M., Yoshizawa, A., Imanishi, T., Taniguchi, H., Inaba, K
  • Journal Title: Nucleic Acids Res. Volume: 39
  • NAID: 120002834765
  • Peer Reviewed
• [Journal Article] Ciona intestinalis Noto4 contains a phosphotyrosine interaction domain and is involved in the midline intercalation of notochord cells. (2011)
  • Author(s): Yamada, S., Ueno, N., Satoh, N., Takahashi, H.
  • Journal Title: Int.J.Dev.Biol. Volume: 55 Pages: 11-18
  • Peer Reviewed
• [Journal Article] Regulation of notochord-specific expression of Ci-Bra downstream genes in Ciona intestinalis embryos. (2010)
  • Author(s): Takahashi H., Hotta K., Takagi C., Ueno N., Satoh N., Shoguchi E.
  • Journal Title: Zool.Sci. 27 Pages: 110-118
  • NAID: 40017358561
  • Peer Reviewed
• [Journal Article] Novel peripheral neuron network in the tunic of normal ascidian revealed by confocal laser scanning and transmission electron microscopies. (2010)
  • Author(s): Terakubo H., Nakajima Y., Sasakura Y., Horie T., Konno A., Takahashi H., Inaba K., Hotta K., Oka K.
  • Journal Title: Dev.Dynamics. 239 Pages: 2278-2287
  • Peer Reviewed
• [Journal Article] Novel peripheral neuron network in the tunic of normal ascidian revealed by confocal laser scanning and transmission electron microscopies. (2010)
  • Author(s): Terakubo, H., Nakajima, Y., Sasakura, Y., Horie, T., Konno, A., Takahashi, H., Inaba, K., Hotta, K., Oka, K.
  • Journal Title: Dev.Dynamics. Volume: 239 Pages: 2278-2287
  • Peer Reviewed
• [Journal Article] Regulation of notochord-specific expression of Ci-Bra downstream genes in Ciona intestinalis embryos (2010)
  • Author(s): Takahashi, H., Hotta, K., Takagi, C., Ueno, N., Satoh, N., Shoguchi, E.
  • Journal Title: Zool.Sci. 27 Pages: 110-118
  • NAID: 40017358561
  • Peer Reviewed
• [Journal Article] Ciona intestinalis Noto4 contains a phosphotyrosine interaction domain and is involved in the midline intercalation of notochord cells (2010)
  • Author(s): Yamada, S., Ueno, N., Satoh, N., Takahashi, H.
  • Journal Title: Int.J.Dev.Biol. (in press)
  • Peer Reviewed
• [Journal Article] Interaction of notochord-derived fibrinogen-like protein with Notch regulates the patterning of the central nervous system of Ciona intestinalis embryos. (2009)
  • Author(s): Yamada S., Hotta K., Yamamoto T.S., Ueno N., Satoh N., Takahashi H.
  • Journal Title: Dev.Biol. 328 Pages: 1-12
  • Peer Reviewed
• [Journal Article] Interaction of notochord-derived fibrinogen-like protein with Notch reg ulates the patterning of the central nervous system of Ciona intestinalis embryos (2009)
  • Author(s): Yamada, S., Hotta, K., Yamamoto, T. S., Ueno, N., Satoh, N., Takahashi, H.
  • Journal Title: Dev. Biol. 328 Pages: 1-12
  • Peer Reviewed
• [Journal Article] Brachyury-downstream gene sets in a chordate, Ciona intestinalis : Integrating notochord specification, morphogenesis and chordate evolution. (2008)
  • Author(s): Hotta K., Takahashi H., Satoh N., Gojobori T.
  • Journal Title: Evo.Dev. 10 Pages: 37-51
  • Peer Reviewed
• [Journal Article] Brachyury-downstream gene sets in a chordate, Ciona intestinalis : Integrating notochord specification, morphogenesis and chordate evolution (2008)
  • Author(s): Hotta, K., Takahashi, H., Satoh, N., Gojobori, T.
  • Journal Title: Evo. Dev. 10 Pages: 37-51
  • Peer Reviewed
• [Presentation] ナメクジウオカルシトニンからみたカルシトニンファミリーの進化機構 (2010)
  • Author(s): 関口俊男、高橋弘樹、小笠原道生、佐竹炎
  • Organizer: 日本比較内分泌学会第35回大会
  • Place of Presentation: 静岡
  • Year and Date: 2010-11-19
• [Presentation] ホヤ幼生末梢神経から被嚢内へ伸びた突起が形成するネットワーク構造の解析 (2010)
  • Author(s): 堀田耕司、寺久保洋、中島陽子、紺野在、堀江健生、高橋弘樹、笹倉靖徳、稲葉一男、岡浩太郎
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-23
• [Presentation] Regulation of notochord-specific expression of Ci-Bra downstream genes in Ciona intestinalis embryos. (2010)
  • Author(s): Takahashi H., Hotta K., Takagi C., Ueno N., Satoh N., Shoguchi E.
  • Organizer: The 16th International Conference of the International Society of Differentiation From Stem Cells to Organisms 2010
  • Place of Presentation: Nara, Japan.
• [Presentation] Regulation of notochord-specific expression of Ci-Bra downstream genes in Ciona intestinalis embryos. (2010)
  • Author(s): Takahashi, H., Hotta, K., Takagi, C., Ueno, N., Satoh, N., Shoguchi, E.
  • Organizer: The 16th International Conference of the International Society of Differentiation
  • Place of Presentation: 奈良県新公会堂(奈良)
• [Presentation] カタユウレイボヤ脊索遺伝子(Ci-Noto4)は脊索鞘の形成に関与する. (2009)
  • Author(s): 山田成宏、上野直人、佐藤矩行、高橋弘樹
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-17
• [Presentation] カタユウレイボヤ脊索遺伝子(Ci-Noto4)は脊索鞘の形成に関与する (2009)
  • Author(s): 山田成宏、上野直人、佐藤矩行、高橋弘樹
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡グランシップ
  • Year and Date: 2009-09-17
• [Presentation] The structure and evolution of caudal epidermal neurons in ascidian larva. (2009)
  • Author(s): Terakubo H., Takahashi H., Nakajima Y., Sasakura Y., Horie T., Hotta K., Oka K.
  • Organizer: 5th International tunicate Meeting
  • Place of Presentation: Okinawa, Japan.
• [Presentation] Interaction of notochord-derived fibrinogen-like protein with Notch regulates the patterning of the central nervous system of Ciona intestinalis embryos. (2008)
  • Author(s): Yamada S., Hotta K., Yamamoto T.S., Ueno N., Satoh N., Takahashi H.
  • Organizer: The 8th NIBB-EMBL joint Meeting, Evolution : Genomes, Cell Types and Shapes
  • Place of Presentation: Okazaki, Japan.
• [Presentation] Brachyury-downstream notochord genes in the chordate evolution.
  • Author(s): 高橋弘樹
  • Organizer: Guest Speaker Seminar, Sars International Center for Marine Molecular Biology
  • Place of Presentation: Bergen, Norway.
• [Remarks] ホームページ等
  • URL: http://www.nibb.ac.jp/morphgen/summary/hoya.html
• [Remarks]
  • URL: http://www.nibb.ac.jp/morphgen/summary/hoya.html