| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
The prevalent allergen of common buckwheat (Fagopyrum esculentum Moench), Fage 1, is β polypeptide of the most abundant storage protein, 13S globulin. Because the13S globulin is composed of multiple diversified subunits, the subunits of 13S globulinwere characterized. The 13S globulin α polypeptides were categorized into four typesand were further grouped into methionine-poor and methionine-rich subunits as major andminor types, respectively. The large variation in size among the α polypeptides was explained by the different lengths of the tandem repeats. Beside the three known methionine-poor subunits of 13S globulin, four new methionine-poor subunits with 0, 2, 4, and 6 tandem repeats seemed to exist. Because the tandem repeat region was hydrophilic with many arginine residues, digestibilities against trypsin were different between the subunits with and without the tandem repeat. The tandem repeat insertion in the methionine-poor subunits of the 13S globulin may reduce common buckwheat allergenicity.
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