Analysis of novel regulatory mechanism of apoptosis mediated by the posttranslational N-myristoylation of protein
| Project/Area Number |
20580099
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Yamaguchi University |
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Principal Investigator |
UTSUMI Toshihiko Yamaguchi University, 大学院・医学系研究科, 教授 (20168727)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 翻訳後N-ミリストイル化 / アポトーシス / 脂質修飾 / ゲルゾリン / アクチン / 翻訳後修飾 / N-ミリストイル化 / 細胞情報伝達 |
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| Research Abstract |
Protein N-myristoylation has been recognized as a cotranslational protein modification. Recently, it was demonstrated that protein N-myristoylation could also occur posttranslationally during apoptosis as in the case of BID, actin, gelsolin and PAK2. The posttranslational N-myristoylation has been shown to play a crucial role in the biological activity of BID, gelsolin, and PAK2. However, it was not fully accepted that the reaction is a universal posttranslational modification frequently observed during the apoptosis pathway.
In this study, we established the strategy for genome-wide screening of posttranslationally N-myristoylated proteins. Using this strategy, we could successfully demonstrate that the posttranslational myristoylation of caspase cleavage product represents a universal mechanism widely used to regulate cellular apoptosis.
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Report
(4 results)
Research Products
(74 results)
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[Journal Article] Preparation of ubiquitin-conjugated proteins using an insect cell-free protein synthesis system.2010
Author(s)
Suzuki, T., Ezure, T., Ando, E., Nishimura, O., Utsumi t., Tsunasawa, S.
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Journal Title
Journal of Biotechnology 145
Pages: 73-78
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Peer Reviewed
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[Journal Article] Alpha-tocopheryl succinate induces rapid and reversible phosphatidylserine external- ization in histiocytic lymphoma through the caspase- independent pathway.2010
Author(s)
Fujita, H., Shiba, D., Utsumi T., Ogino, T., Ogawa, T., Yasuda, T., Utsumi, K., Sasaki, J.
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Journal Title
Molecular and Cellular Biochemistry 333
Pages: 137-148
Related Report
Peer Reviewed
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[Journal Article] Development of an Insect Cell-Free System.2010
Author(s)
Ezure, T., Suzuki, T., Shikata, M., Ito, M., Ando, E., Utsumi T., Nishimura, O., Tsunasawa, S.
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Journal Title
Current Pharmaceutical Biotechnology 11
Pages: 279-284
Related Report
Peer Reviewed
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[Journal Article] Strategy for comprehensive identification of human N-myristoylated proteins using an insect cell-free protein synthesis system.2010
Author(s)
Suzuki, T., Moriya, K., Nagatoshi, K., Ohta, Y., Ezure, T., Ando, E., Tsunasawa, S., Utsumi T.
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Journal Title
Proteomics 10
Pages: 1780-1793
Related Report
Peer Reviewed
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[Journal Article] Bombyx mori Ras proteins, BmRas1, BmRas2 and BmRas3 are neither farnesylated nor palmitoylated but are geranylgeranylated.2010
Author(s)
Moriya, K., Tsubota, T., Ishibashi, N., Yafune, A., Suzuki, T., Kobayashi, J., Shiotsuki, T., Utsumi T.
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Journal Title
Insect Molecular Biology 19
Pages: 291-301
Related Report
Peer Reviewed
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[Journal Article] Characterization of the consensus motif for N-myristoylation of plant proteins with a cell-free system based on wheat germ extract.2010
Author(s)
Yamauchi, S., Fusada, N., Hayashi, H., Utsumi T., Uozumi, N., Endo, Y., Tozawa, Y.
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Journal Title
FEBS Journal 277
Pages: 3579-3607
Related Report
Peer Reviewed
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[Journal Article] Mechanism of cell death by 5-aminolevulinic acid-based photodynamic action and its enhancement by Mn^<2+>, deferoxamine, and NOC-18 in human histiocytic lymphoma cell line U937.2009
Author(s)
Amo, T., Kawanishi, N., Uchida, M., Fujita, H., Oyanagi, E., Utsumi T., Inoue, K., Shuin, T., Utsumi, K., Sasaki, J.
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Journal Title
Cell Biochemistry and Function 27
Pages: 503-515
Related Report
Peer Reviewed
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[Journal Article] Alpha-Lipoic acid suppresses 6-hydroxydopamine-induced ROS generation and apoptosis through the stimulation of glutathione synthesis but not by the expression of heme oxygenase-1.2008
Author(s)
Fujita, H., Shiosaka, M., Ogino, T., Okimura, Y., Utsumi T., Sato, E., Akagi, R., Inoue, M., Utsumi, K., Sasaki, J.
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Journal Title
Brain Res. 1206
Pages: 1-12
Related Report
Peer Reviewed
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[Presentation] Analysis of lipid modification occurs on Bombyx mori Ras proteins, BmRas1, BmRas2, and BmRas3.2009
Author(s)
守屋康子, 坪田拓也, 石橋直人, Yafune, A., 鈴木崇, 小林淳, 塩月孝博, 内海俊彦
Organizer
IUBMB 2009
Place of Presentation
上海(中国)Shanghai International Convention Center
Year and Date
2009-08-04
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