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Evaluation of freeze-dried bull spermatozoa by intra-or inter-species micro insemination

Research Project

Project/Area Number 20580305
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionShinshu University

Principal Investigator

HOCHI Shinichi  信州大学, 繊維学部, 教授 (10283243)

Project Period (FY) 2008 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsウシ精子 / 顕微授精 / 凍結乾燥 / カルシウムオシレーション / DNA脱メチル化 / ROCK阻害剤 / ガラス化保存 / 微小管形成中心 / 精子中心体 / コメットアッセイ / 胚盤胞 / 共焦点レーザー顕微鏡 / 超急速ガラス化保存 / 卵子活性化
Research Abstract

The purpose of this study was to investigate whether freeze-drying process of bull spermatozoa disturbed early post-fertilization events essential to gain the developmental competence of intracytoplasmically sperm-injected(ICSI) oocytes. In interspecies assay using mouse ovulated oocytes, the ability of freeze-dried(FD) bull spermatozoa to induce normal intracellular calcium oscillation pattern was comparable despite of temperatures stored for 1 year. DNA fragmentation in FD spermatozoa stored at+ 4℃was not detected by alkaline comet assay. With intraspecies(bovine-bovine) ICSI, the FD process was found to be innocent in performance of epigenetic remodeling(active DNA demethylation) and function of microtubule-organizing center(sperm aster formation and network assembly) in the pronuclear-stage zygotes. Factors responsible for low blastocyst yield from the FD-ICSI bovine oocytes remain to be determined.

Report

(6 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (39 results)

All 2011 2010 2009 2008

All Journal Article (19 results) (of which Peer Reviewed: 19 results) Presentation (18 results) Book (2 results)

  • [Journal Article] Procedure for bovine ICSI, not spermfreeze-drying, impairs the function of the microtubule-organizing center.2011

    • Author(s)
      H. Hara, H. Abdalla, H. Morita, M. Kuwayama, M. Hirabayashi, S. Hochi.
    • Journal Title

      J Reprod Dev誌

      Volume: 57巻 Pages: 428-432

    • NAID

      10029050914

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Challenging endeavourfor preservation of freeze-dried mammalian spermatozoa2011

    • Author(s)
      S. Hochi, H. Abdalla, H. Hara, M. Hirabayashi
    • Journal Title

      J Reprod Dev誌

      Volume: 57巻 Pages: 557-563

    • NAID

      10029878056

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Procedure for Bovine ICSI, not Sperm Freeze-drying, Impairs the Function of the Microtubule-organizing Center2011

    • Author(s)
      Hiromasa Hara
    • Journal Title

      Journal of Reproduction and Development

      Volume: 57 Issue: 3 Pages: 428-432

    • DOI

      10.1262/jrd.10-167N

    • NAID

      10029050914

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Challenging Endeavour for Preservation of Freeze-Dried Mammalian Spermatozoa2011

    • Author(s)
      Shinichi Hochi
    • Journal Title

      Journal of Reproduction and Development

      Volume: 57 Issue: 5 Pages: 557-563

    • DOI

      10.1262/jrd.11-061O

    • NAID

      10029878056

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vitrification of ICSI-and IVF-derived bovine blastocysts by minmum volume cooling procedure : effect of developmental stage and age2010

    • Author(s)
      H. Abdalla, M. Shimoda, H. Hara, H. Morita, M. Kuwayama, M. Hirabayashi, S. Hochi
    • Journal Title

      Theriogenology誌

      Volume: 74巻 Pages: 1028-1035

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stimulatory effect of Rhoassociated coiled-coil kinase(ROCK) inhibitor on revivability of in vitroproduced bovine blastocysts after vitrification2010

    • Author(s)
      S. Hochi, H. Abdalla, H. Hara, M. Shimoda, H. Morita, M. Kuwayama, M. Hirabayashi
    • Journal Title

      Theriogenology誌

      Volume: 73巻 Pages: 1139-1145

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vitrification of ICSI-and IVF-derived bovine blastocysts by minmum volume cooling procedure : effect of developmental stage and age.2010

    • Author(s)
      H.AbdaRa, M.Shimoda, H.Hara, H.Morita, M.Kuwayama, M.Hirabayashi, S.Hochi.
    • Journal Title

      Theriogenology誌 74巻

      Pages: 1028-1035

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Stimulatory effect of Rho-associated coiled-coil kinase (ROCK) inhibitor on revivability of in vitro-produced bovine blastocysts after vitrification.2010

    • Author(s)
      S.Hochi, H.Abdalla, H, Hara, M。Shimoda, H.Morita, M.Kuwayama, M.Hirabayashi.
    • Journal Title

      Theriogenology誌 73巻

      Pages: 1139-1145

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Stimulatory effect of Rho-associated coiled-coil kinase(ROCK)inhibitor on revivabilitv of in vitro-produced bovine blastocysts after vitrification2010

    • Author(s)
      Shinichi Hochi
    • Journal Title

      Theriogenology

      Volume: 73 Pages: 1139-1145

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vitrification of ICSI-and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age2010

    • Author(s)
      Hany Abdalla
    • Journal Title

      Theriogenology

      Volume: 74 Pages: 1028-1035

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A combined treatment of ionomycin with ethanol improve sblastocyst development of bovineoocytes harvested from stored ovaries and microinjected with sperma-tozoa2009

    • Author(s)
      H. Abdalla, M. Shimoda, M. Hirabayashi, S. Hochi
    • Journal Title

      Theriogenology誌

      Volume: 72巻 Pages: 453-460

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Demethylation dynamics of thepaternal genome in pronuclear-stagebovine zygotes produced by in vitrofertilization and ooplasmic injection offreeze-thawed or freeze-dried spermatozoa2009

    • Author(s)
      H. Abdalla, M. Hirabayashi, S. Hochi
    • Journal Title

      J Reprod Dev誌

      Volume: 55巻 Pages: 433-439

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The ability of freeze-dried bull spermatozoa to induce calcium oscillations and resumption of meiosis2009

    • Author(s)
      H. Abdalla, M. Hirabayashi, S. Hochi
    • Journal Title

      Theriogenology誌

      Volume: 71巻 Pages: 543-552

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with sperma-tozoa.2009

    • Author(s)
      H.Abdalla, M.Shimoda, M.Hirabayashi, S.Hochi.
    • Journal Title

      Theriogenology誌 72巻

      Pages: 453-460

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Demethylation dynamics of the paternal genome in pronuclear-stage bovine zygotes produced by in vitro fertilization and ooplasmic injection of freeze-thawed or freeze-dried spermatozoa.2009

    • Author(s)
      H.Abdalla, M.Hirabayashi, S.Hochi
    • Journal Title

      J Reprod Dev誌 55巻

      Pages: 433-439

    • NAID

      10025194467

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] The ability of freeze-dried bull sperma-tozoa to induce calcium oscillations and resumption of meiosis.2009

    • Author(s)
      H.Abdalla, M.Hirabayashi, S.Hochi.
    • Journal Title

      Theriogenology誌 71巻

      Pages: 543-552

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Demethylation dynamics of the paternal genome in pronuclear-stage bovine zygotes produced by in vitro fertilization and ooplasmic injection of freeze-thawed or freeze-dried spermatozoa2009

    • Author(s)
      Hany ABDALLA
    • Journal Title

      Journal of Reproduction and Development 55

      Pages: 433-439

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa2009

    • Author(s)
      Hany ABDALLA
    • Journal Title

      Theriogenology 72

      Pages: 453-460

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The ability of freeze-dried bull spermatozoa to induce calcium oscillations and resumption of meiosis2009

    • Author(s)
      Hany ABDALLA
    • Journal Title

      Theriogenology 71

      Pages: 543-552

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 顕微授精、精子凍結乾燥、卵子ガラス化の各行程がウシ微小管形成中心の機能発現に及ぼす影響2011

    • Author(s)
      原弘真
    • Organizer
      第52回日本哺乳動物卵子学会
    • Place of Presentation
      栃木/大田原
    • Year and Date
      2011-05-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] 顕微授精、精子凍結乾燥、卵子ガラス化の各行程がウシ微小管形成中心の機能発現に及ぼす影響2011

    • Author(s)
      原弘真
    • Organizer
      第52回日本哺乳動物卵子学会
    • Place of Presentation
      栃木
    • Related Report
      2011 Final Research Report
  • [Presentation] IVFまたはICSIに由来するウシ前核期卵におけるDNAメチル化量とH3ヒストンアセチル化量との関係2010

    • Author(s)
      原弘真
    • Organizer
      第103回日本繁殖生物学会
    • Place of Presentation
      青森
    • Related Report
      2011 Final Research Report
  • [Presentation] ICSIとIVFに由来するウシ胚盤胞の超急速ガラス化保存:発生速度と発育ステージの影響2010

    • Author(s)
      下田美怜
    • Organizer
      第51回日本哺乳動物卵子学会
    • Place of Presentation
      新潟
    • Related Report
      2011 Final Research Report 2010 Annual Research Report 2010 Self-evaluation Report
  • [Presentation] ROCKの活性阻害はガラス化保存した体外受精由来ウシ胚盤胞の蘇生率を改善する2010

    • Author(s)
      原弘真
    • Organizer
      第112回日本畜産学会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report 2010 Self-evaluation Report
  • [Presentation] ICSIとIVFにより作出した拡張度が異なる7日目ウシ胚盤胞におけるガラス化耐性の比較2010

    • Author(s)
      下田美怜
    • Organizer
      第112回日本畜産学会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] IVFまたはICSIに由来するウシ前核期卵におけるDNAメチル化量とH3ヒストンアセチル化量との関係2010

    • Author(s)
      原弘真
    • Organizer
      第103回日本繁殖生物学会
    • Place of Presentation
      十和田
    • Related Report
      2010 Annual Research Report 2010 Self-evaluation Report
  • [Presentation] ウシ卵細胞質内精子注入法の効率改善と凍結乾燥精子を用いた胚盤胞作製の試み2009

    • Author(s)
      下田美怜
    • Organizer
      第102回日本繁殖生物学会
    • Place of Presentation
      奈良
    • Related Report
      2011 Final Research Report
  • [Presentation] ウシ顕微授精胚の胚盤胞発生率を改善する卵子活性化補填法について2009

    • Author(s)
      保地眞一
    • Organizer
      第110回日本畜産学会
    • Place of Presentation
      神奈川
    • Related Report
      2011 Final Research Report 2008 Annual Research Report
  • [Presentation] Demethylation dynamics ofpaternal genomes in pronuclear bovinezygotes produced by IVF and ICSI withnon-dried or freeze-dried spermatozoa2009

    • Author(s)
      Abdalla H
    • Organizer
      第35回国際胚移植学会
    • Place of Presentation
      カリフォルニア州
    • Related Report
      2011 Final Research Report
  • [Presentation] Demethylation dynamics of paternal genomes in pronuclear bovine zygotes produced by IVF and ICSI with non-dried or freeze-dried spermatozoa2009

    • Author(s)
      Abdalla H
    • Organizer
      第35回国際胚移植学会
    • Place of Presentation
      カリフォルニア州
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] ウシ卵細胞質内精子注入法の効率改善と凍結乾燥精子を用いた胚盤胞作製の試み2009

    • Author(s)
      下田美怜
    • Organizer
      第102回日本繁殖生物学会
    • Place of Presentation
      近畿大学農学部(奈良)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Demethylation dynamics of paternal genomes in pronuclear bovine zygotes produced by IVF and ICSI with non-dried or freeze-dried spermatozoa2009

    • Author(s)
      ハニー アブダツラ
    • Organizer
      第35回国際胚移植学会
    • Place of Presentation
      アメリカ、カリフォルニア州
    • Related Report
      2008 Annual Research Report
  • [Presentation] Demethylation dynamics ofpaternal genomes in pronuclear bovinezygotes produced by IVF and ICSI withnon-dried or freeze-dried spermatozoa2008

    • Author(s)
      Abdalla H
    • Organizer
      第101回日本繁殖生物学会
    • Place of Presentation
      福岡
    • Related Report
      2011 Final Research Report
  • [Presentation] Ability of freeze-dried bullspermatozoa to induce calcium oscillations and meiosis resumption2008

    • Author(s)
      Abdalla H
    • Organizer
      第1回世界生殖生物工学会議
    • Place of Presentation
      ハワイ州
    • Related Report
      2011 Final Research Report
  • [Presentation] Ability of freeze-dried bull spermatozoa to induce calcium oscillations and meiosis resumption2008

    • Author(s)
      Abdalla H
    • Organizer
      第1回世界生殖生物工学会議
    • Place of Presentation
      ハワイ州
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] Ability of freeze-dried bull spermatozoa to induce calcium oscillations and meiosis resumption2008

    • Author(s)
      ハニー アブダツラ
    • Organizer
      第1回世界生殖生物工学会議
    • Place of Presentation
      アメリカ、ハワイ州
    • Related Report
      2008 Annual Research Report
  • [Presentation] Demethylation dynamics of paternal genomes in pronuclear bovine zygotes produced by IVF and ICSI with non-dried or freeze-dried spermatozoa2008

    • Author(s)
      ハニー アブダツラ
    • Organizer
      第101回日本繁殖生物学会
    • Place of Presentation
      福岡
    • Related Report
      2008 Annual Research Report
  • [Book] Vitro Fertilization2011

    • Author(s)
      S. Hochi, H. Abdalla, M. Hirabayashi
    • Publisher
      Nova Science Publishers
    • Related Report
      2011 Final Research Report
  • [Book] In Vitro Fertilization/Intracytoplasmic sperm injection in cattle2011

    • Author(s)
      Shinichi Hochi
    • Publisher
      Nova Science Publishers
    • Related Report
      2011 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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