Development of cell differentiation against malignant melanoma.

• Project/Area Number: 20580352
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Tottori University
• Principal Investigator: OKAMOTO Yoshiharu Tottori University, 農学部, 教授 (50194410)
• Co-Investigator(Kenkyū-buntansha): HATA Keishi 秋田県農林水産技術センター総合食品研究所, 食品機能グループ, 主任研究員 (80360353) ; OGIHARA Kikumi 麻布大学, 環境保健学部, 講師 (50154381)
• Co-Investigator(Renkei-kenkyūsha): MINAMI Saburou 鳥取大学, 農学部, 教授 (70032307) ; TSUKA Takeshi 鳥取大学, 農学部, 准教授 (30432610)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: メラノーマ / 分化誘導 / イヌ / 遊走能 / B16 2F2 / チロシナーゼ / MITF / Rab27a

Research Abstract

In the present study, we could elucidate the mechanism that lupeol induces differentiation of melanoma cell in vitro. In the gene analysis, lupeol affects cell cycle. In vivo study using mice, it was found that lupeol inhibits growth of melanoma by local and systemic administrations. Furthermore, it was found that lupeol is effective for naturally occurring melanomas in dogs.

Research Products

• [Journal Article] Effects of Lupeol on Melanoma In Vitro and In Vivo : Fundamental and Clinical Trials. (2010)
  • Author(s): Keishi Hata, Kikumi Ogihara, Saori Takahashi, Takeshi Tsuka, Saburo Minami, Yoshiharu Okamoato
  • Journal Title: Animal Cell Technology : Basic & Applied Aspects 16 Pages: 339-344
  • Peer Reviewed
• [Journal Article] Melanoma cell differentiation induced by lupeol separates into two stages : morphological and functional changes. (2009)
  • Author(s): Kikumi Ogiwara, Keishi Hata.
  • Journal Title: J.Nat.Med. 63 Pages: 323-326
  • NAID: 10025826562
  • Peer Reviewed
• [Journal Article] Differentiation-inducing activities by lupane triterpenes from Lactuca indica on a mouse melanoma cell line. (2009)
  • Author(s): Keishi Hata, Toshiyuki Mukaiyama, Noriyuki Tsujimura, Yusuke Sato, Yasuyuki Kosaka, Kenji Sakamoto, Kazuyuki Hori.
  • Journal Title: Animal Cell Technology : Basic & Applied Aspects 15 Pages: 279-285
  • Peer Reviewed
• [Journal Article] Melanoma cell differentiation induced by lupeol separates into two stages : morphological and functional changes. (2009)
  • Author(s): Ogiwara, K., Hata, K.
  • Journal Title: J. Nat. Med (in press)
  • NAID: 10025826562
  • Peer Reviewed
• [Presentation] 悪性黒色腫に対するルペオールの基礎的検討と臨床応用 (2011)
  • Author(s): 岡本芳晴、松島伸昭、柄武志、新田牧子、今川智敬、大崎智弘、南三郎
  • Organizer: 平成22年度日本獣医師会学会年次大会
  • Place of Presentation: 岐阜市
  • Year and Date: 2011-02-11
• [Presentation] 悪性黒色腫に対するlupeolの効果 (2009)
  • Author(s): 新田牧子、柄武志、今川智敬、南三郎、畠恵司、荻原喜久美、岡本芳晴
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取市
  • Year and Date: 2009-09-26
• [Presentation] キク科植物由来ルペオールのメラノーマ細胞分化誘導活性 (2009)
  • Author(s): 畠恵司、高橋沙織、荻原喜久美、柄武志、南三郎、岡本芳晴
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取市
  • Year and Date: 2009-09-26
• [Presentation] 悪性黒色腫に対するlupeo1の効果 (2009)
  • Author(s): 新田牧子、柄武志、今川智敬、南三郎、畠恵司、荻原喜久美、岡本芳晴
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取市
  • Year and Date: 2009-09-26
• [Presentation] Effects of lupeol on melanoma in vitro and in vivo-Fundamental and clinical trials-. (2008)
  • Author(s): 畠恵司, 荻原喜久美, 高橋沙織, 柄武志, 南三郎, 岡本芳晴
  • Organizer: The 21st Annual and International Meeting of Japanese Association for Animal Cell Technology (JAACT 2008)
  • Place of Presentation: 福岡市
  • Year and Date: 2008-11-26
• [Presentation] EFFECTS OF LUPEOL ON MELANOMA IN VITRO AND IN VIVO-FUNDAM ENTAL AND CLINICAL TRIALS- (2008)
  • Author(s): Keishi Hata, Kikumi Ogihara, Saori Takahashi, Takeshi Tsuka, Saburo Minami, Yoshiharu Okamoto
  • Organizer: The 21st Annual and International Meeting of Japanese Association for Animal Cell Technology (JAACT 2008)
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-11-26
• [Book] Animal Cell Technology : Basic & Applied Aspects (2010)
  • Author(s): Hata, K., Ogihara, K., Takahashi, S., Tsuka, T., Minami, S., Okamoto, Y.
  • Total Pages: 6
  • Publisher: Springer Science+Business Media B.V.