Production of hepatocyte growth factor (HGF) and tissue regeneration induced by natural products

• Project/Area Number: 20590058
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Okayama University
• Principal Investigator: GOHDA Eiichi Okayama University, 大学院・医歯薬学総合研究科, 教授 (20028814)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 肝細胞増殖因子(HGF) / 細胞増殖 / ニガウリ / 冬虫夏草 / ポリミキシンB / ヒト真皮線維芽細胞 / サイクリックAMP / 真皮線維芽細胞 / ERK

Research Abstract

Hepatocyte growth factor (HGF) has been shown to stimulate the proliferation of epithelial cells in various tissues including hepatocytes and play critical roles in regenerative events of tissues. Here we found that the extracts of bitter melon placenta and Cordyceps sinensis and polymyxin B promoted HGF production in human fibroblasts and described their mechanism and properties of active component(s). Injection of polymyxin B to rats significantly increased both plasma and hepatic HGF levels.

Research Products

• [Journal Article] Induction of hepatocyte growth factor production in human dermal fibroblasts and their proliferation by the extract of bitter melon pulp. (2009)
  • Author(s): Ono, T., Tsuji, T., Sakai, M., Yukizaki, C., Ino, H., Akagi, I., Hiramatsu, K., Matsumoto, Y., Sugiura, Y., Uto, H., Tsubouchi, H., Gohda, E.
  • Journal Title: Cytokine 46巻 Pages: 119-126
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 合田榮一
  • Journal Title: 壮快(ゴーヤに傷の治りを早くする成分があると実験で判明)(マキノ出版) Pages: 98-99
• [Journal Article] Induction of hepatocyte growth factor production in human dermal fibroblasts and their proliferation by the extract of bitter melon pulp. (2009)
  • Author(s): Ono, T., Tsuji, T., Sakai, M., Yukizaki, C., Ino, H., Akagi, I., Hiramatsu, K., Matsumoto, Y., Sugiura, Y., Uto, H., Tsubouchi, H., Gohda, E.
  • Journal Title: Cytokine 46 Pages: 119-126
  • Peer Reviewed
• [Presentation] ポリミキシンBによるヒト皮膚線維芽細胞の増殖誘導 (2009)
  • Author(s): 土手口修平、浜渦玲美、木村洸、合田榮一
  • Organizer: 第48回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
  • Place of Presentation: 徳島市
  • Year and Date: 2009-11-07
• [Presentation] ポリミキシンBによるヒト皮膚線維芽細胞の増殖誘導 (2009)
  • Author(s): 合田榮一
  • Organizer: 薬学会
  • Place of Presentation: 徳島市
  • Year and Date: 2009-11-07
• [Presentation] ニガウリ抽出物のヒト真皮線維芽細胞における増殖促進作用 (2009)
  • Author(s): 小野雄大、齋藤大介、小林かほり、辻友絵、酒井美穂、柚木崎千鶴子、井野寿俊、赤木功、坪内博仁、合田榮一
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都市
  • Year and Date: 2009-03-26
• [Remarks] 合田榮一、ゴーヤに傷の治りを早くする成分があると実験で判明、壮快、2009、98-99、マキノ出版