The structure and function of new drug excreting transporter "MATE" in mannmal.

• Project/Area Number: 20590059
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Okayama University
• Principal Investigator: OOTSUKA Masato Okayama University, 自然生命科学研究支援センター, 准教授 (30243489)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 生化学 / 薬物排出 / 輸送体 / 有機カチオン / 薬物副作用軽減 / 多剤耐性 / 薬物輸送 / MATE / プロトン交換輸送系 / Kidney / Transporter / Organic cation exchange / multidrug / Multidrug transporter / organic cation transporter / kidney

Research Abstract

There are so many transporters in kidney and these transporters are concerned about transepithelial transport. These transporters don't work alone but may be working together. From this idea, drug excretion is precisely controlled and these should be well organized mechanism. MATE transporter is also controlled with these mechanism. In MATE transporter, there is long tail in C-terminus region, so, in this region should hove some function for regulation. From this hypothesis, several functional proteins are identified.

Research Products

• [Journal Article] Characterization of the human MATE2 proton-coupled polyspecific organic cation exporter. (2011)
  • Author(s): Otsuka M, Komatsu T, Hiasa M, Kanamoto T, Matsumoto T, Moriyama Y, Omote H.
  • Journal Title: Int J Biochem Cell Biol. 43(6) Pages: 913-918
• [Journal Article] Characterization of the human MATE2 proton-coupled polyspecific organic cation exporter. (2011)
  • Author(s): Otsuka M, Komatsu T, Hiasa M, Kanamoto T, Matsumoto T, Moriyama Y, Omote H.
  • Journal Title: Int J Biochem Cell Biol. Volume: 43(6) Pages: 913-918
  • Peer Reviewed
• [Journal Article] Identification of a vesicular nucleotide transporter. (2009)
  • Author(s): Sawada K, Echigo N, Juge N, Miyaji T, Otsuka M, Omote H, Yamamoto A, Moriyama Y.
  • Journal Title: Proc Natl Acad Sci USA(National Academy of Sciences) Vol.105, No.15 Pages: 5683-5686
• [Journal Article] Identification of a vesicular nucleotide transporter. (2009)
  • Author(s): Sawada K, Echigo N, Juge N, Miyaji T, Otsuka M, Omote H, Yamamoto A, Moriyama Y.
  • Journal Title: Proc Natl Acad Sri USA. 105 Pages: 5683-5686
  • Peer Reviewed
• [Journal Article] A novel variant of mouse MATE-1 H+/organic cation antiporter with a long hydrophobic tail. (2008)
  • Author(s): Kobara A. Hiasa M, Matsumoto T, Otsuka M. Omote H. Moriyama Y.
  • Journal Title: Arch Biochem Biophys 469(2) Pages: 195-199
  • Peer Reviewed
• [Journal Article] Role of glutamate residues in substrate recognition by human MATEl polyspecific H+/organic cation exporter (2008)
  • Author(s): Matsumoto T, Kanamoto T, Otsuka M, Omote H, Moriyama Y.
  • Journal Title: Am J Physiol Cell Physiol. 294(4) Pages: 1074-1078
  • Peer Reviewed
• [Journal Article] Identification of a vesicular nucleotide transporter. (2008)
  • Author(s): Sawada K, Echigo N. Juge N, Miyaji T, Otsuka M, Omote H, Yamamoto A, Moriyama Y.
  • Journal Title: Proc Natl Acad Sci U S A. 105(15) Pages: 5683-5686
  • Peer Reviewed
• [Presentation] ヒトMATE型のトランスポートソーム解析 (2011)
  • Author(s): 大塚正人、小田昌幸
  • Organizer: 日本薬学会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-27
• [Presentation] ヒトMATE型のトランスポートソーム解析 (2010)
  • Author(s): 小田和昌、大塚正人
  • Organizer: 日本薬学会
  • Place of Presentation: 岡山
• [Presentation] 薬物の最終段階を司る新規輸送体について (2008)
  • Author(s): 大塚正人
  • Organizer: 膜タンパク質シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2008-05-14
• [Presentation] Molecular characterization of the human MATE2 polyspecific H^+/organic cation exporter. (2008)
  • Author(s): 大塚正人、松本拓也、日浅未来、表弘志、森山芳則
  • Organizer: 第81回日本生化学会大会,第31回日本分子生物学会年会,BMB2008
  • Place of Presentation: 神戸
• [Presentation] 小胞型ATPトランスポーターの同定と機能解析 (2008)
  • Author(s): 澤田啓介、越後典子、樹下成信、宮地孝明、日浅未来、大塚正人、表弘志、森山芳則
  • Organizer: 第30回生体膜と薬物の相互作用シンポジウム,第21回バイオメディカル分析科学シンポジウム
• [Presentation] 小胞型グルタミン酸トランスポーター1(VGLUT1)及びVGLUT1Rの機能解析 (2008)
  • Author(s): 居原田真史、樹下成信、日浅未来、大塚正人、表弘志、森山芳則
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
• [Remarks] ホームページ等
  • URL: http://www.okayama-u.ac.jp/user/grcweb/otsuka/otsuka.html