Expression and biological function of a novel endogenous cholinergic polypeptide in immune cells

• Project/Area Number: 20590094
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Musashino University
• Principal Investigator: KAWASHIMA Koichiro Musashino University, 薬学研究所, 客員教授 (70095008)
• Co-Investigator(Kenkyū-buntansha): HIMI Toshiyuki 武蔵野大学, 薬学研究所, 教授 (30222243)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: SLURP-1 / ニコチン受容体 / 気道上皮細胞 / T細胞 / 抗体 / 喘息 / 抗SLURP-1抗体 / アセチルコリン / α7 nAChR / 細胞増殖 / アセチルコリン産生 / アセチルコリン遊離 / RT-PCR / Western Blotting / 免疫組織化学 / 気管支線毛上皮細胞 / CHT1 / α7nAChR / Western blot / マクロファージ

Research Abstract

SLURP-1 is a novel endogenous peptide with an allosteric agonistic activity for α7 nAChR. Specific expression of SLURP-1 in the bronchial epithelial cells was discovered by an immunohistochemical procedure. SLURP-1 expression in the lung was significantly suppressed in mice with bronchial asthma. SLURP-1 modified differentiation and cholinergic activity of T cells. These findings suggest that SLURP-1 is involved in pathophysiology of asthma and regulation of immune function. We prepared two different kinds of antibodies against human type SLURP-1 in guinea pigs and rabbits in order to develop an ELISA for SLURP-1.

Research Products

• [Journal Article] Down-regulation of secreted lymphocyte antigen-6/urokinase-type plasminogen activator receptor-related peptide-1 (SLURP-1), an endogenous alpha7 nicotinic acetylcholine receptor modulator, in murine and human asthmatic conditions. (2010)
  • Author(s): Narumoto O, Horiguchi K, Horiguchi S, Moriwaki Y, Takano-Ohmuro H, Shoji S, Misawa H, Yamashita N, Nagase T, Kawashima K, Yamashita N
  • Journal Title: Biochem Biophys Res Commun 398 Pages: 713-718
• [Journal Article] Down-regulation of secreted lymphocyte antigen-6/urokinase-type plasminogen activator receptor-related peptide-1(SLURP-1), an endogenous allosteric alpha7 nicotinic acetylcholine receptor modulator, in murine and human asthmatic conditions. (2010)
  • Author(s): Narumoto O, Horiguchi K, Horiguchi S, Moriwaki Y, Takano-Ohmuro H, Shoji S, Misawa H, Yamashita N, Nagase T, Kawashima K, Yamashita N
  • Journal Title: Biochem Biophys Res Commun Volume: 398 Pages: 713-718
  • Peer Reviewed
• [Journal Article] Expression of SLURP-1, an endogenous α7 nicotinic acetylcholine receptor allosteric ligand, in murine bronchial epithelial cells. (2009)
  • Author(s): Horiguchi K, Yamashita N, Horiguchi S, Irie K, Masuda J, Takano-Ohmuro H, Himi T, Miyazawa M, Moriwaki Y, Okuda T, Misawa H, Ozaki H, Kawashima K
  • Journal Title: J Neurosci Res 87 Pages: 2740-2747
• [Journal Article] Expression of SLURP-1, an endogenous α7 nicotinic acetylcholine receptor allosteric ligand, in murine bronchial epithelial cells. (2009)
  • Author(s): Horiguchi K, et al.
  • Journal Title: J Neurosci Res 87 Pages: 2040-2047
  • Peer Reviewed
• [Journal Article] Expression of SLURP-1, an endogenous α7 nicotinic acetylcholine re-ceptor allosteric ligand, in murine bronchial epithelial cells. (2009)
  • Author(s): Horiguchi K, et al.
  • Journal Title: J Neurosci Res (in press)
  • Peer Reviewed
• [Presentation] SLURP-1 up-regulates acetylcholine release in T cells. (2011)
  • Author(s): Fujii T, Takada-Takatori Y, Kawashima K
  • Organizer: The 48^<th> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Kanagawa-ken, Japan
  • Year and Date: 2011-03-23
• [Presentation] SLURP-1 up-regulates acetylcholine release in T cells. (2011)
  • Author(s): Fujii T, Takada-Takatori Y, Kawashima K
  • Organizer: The 48^<th> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Kanagawa-ken
  • Year and Date: 2011-03-23
• [Presentation] Decreased expression of SLURP-1, an endogenous alpha7 nicotinic acetylcholine receptor modulator, in the bronchus of asthmatic mice. (2010)
  • Author(s): Narumoto O, Horiguchi K, Horiguchi S, Moriwaki Y, Takano-Ohmuro H, Shoji S, Misawa H, Yamashita N, Nagase T, Kawashima K, Yamashita N
  • Organizer: Annual Meeting of Society for Neuroscience
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2010-11-17
• [Presentation] Decreased expression of SLURP-1, an endogenous alpha7 nicotinic acetylcholine receptor modulator, in the bronchus of asthmatic mice. (2010)
  • Author(s): Yamashita N, Horiguchi K, Horiguchi S, Moriwaki Y, Misawa H, Kawashima K
  • Organizer: Annual Meeting of Society for Neuroscience
  • Place of Presentation: San Diego, CA, USA.
  • Year and Date: 2010-10-17
• [Presentation] Expression of SLURP-1, an endogenous alpha7 nicotinic acetylcholine receptor modulator, in bronchial epithelial cells. (2009)
  • Author(s): Kawashima K, Horiguchi K, Horiguchi S, Yamashita N, Moriwaki Y, Misawa H
  • Organizer: Annual Meeting of Society for Neuroscience
  • Place of Presentation: Chicago, IL, USA.
  • Year and Date: 2009-10-21
• [Presentation] Expression of SLURP-1, an endogenous alpha7 nicotinic acetylcholine receptor modulator, in bronchial epithelial cells. (2009)
  • Author(s): Kawashima K, et al.
  • Organizer: 39^<th> Annual Meeting of Neuroscience 2009
  • Place of Presentation: Chicago, IL, USA
  • Year and Date: 2009-10-21