| Project/Area Number |
20590163
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Medical pharmacy
|
|---|
| Research Institution | Kinjo Gakuin University |
|---|
Principal Investigator |
HAYASHI Yayoi Kinjo Gakuin University, 薬学部, 教授 (00117847)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
INOUE Katsuhisa 名古屋市立大学, 大学院・薬学研究科, 准教授 (50315892)
HATAKEYAMA Mai 金城学院大学, 薬学部, 助教 (20454340)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | 葉酸 / メトトレキセート / トランスポーター / PCFT / 転写活性化因子 / 転写抑制因子 / エタノール / フラボノイド / hPCFT / 消化管吸収 / 転写因子 / 発現制御 / フラボノイド類 |
|---|
| Research Abstract |
Chronic alcohol consumption have been suspected to cause malabsorption of folates by decreaseing the function of folate transporter(s) in small intestine. Therefore, we examined the effects of ethanol and several flavonoids on the function of proton-coupled folate transporter (PCFT). Pretreatment of Caco-2 cells with myricetin, which is included in wines, caused inhibition of the uptake of folate mediated by PCFT, but not with ethanol. We also examined human PCFT promoter activity with several transcriptional factors to elucidate transcriptional regulation mechanism involved in intestinal PCFT expression. It was found that KLF4 is a major nuclear factor involved in the activation of PCFT promoter, and the KLF4-induced activation is likely to be synergistically regulated by HNF4α, CDX2 and C/EBPα.
|
