Trial to identify membranous protein(s) regulating the planar cell polarity

• Project/Area Number: 20590172
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Ehime University
• Principal Investigator: KOBAYASHI Naoto Ehime University, 大学院・医学系研究科, 教授 (50234836)
• Co-Investigator(Kenkyū-buntansha): HAMADA Fumihiko 大分大学, 医学部, 教授 (70252707)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 発生・分化 / 平面内細胞極性 / 平面内細胞極性形成シグナル伝達 / Frizzled受容体 / Frizzledリガンド / ショウジョウバエS2細胞株 / キメラ受容体分子 / 平面内細胞極性(PCP) / PCP形成シグナル伝達 / Planar Cell Polarity(PCP) / PCP形成シグナル伝達経路 / リガンド

Research Abstract

Planar cell polarity is known to be established mainly by the signals originated from the Frizzled-1 receptor localized on the plasma membrane, although available information on the ligand molecule for Frizzled-1 is still limited. The present study has aimed to reveal the unknown ligand molecule(s) using Drosophila cell culture system coupled with chimeric molecule (Frizzled-1-Frizzled-2 chimeras) technique. We have established culture cell-based assay system of the target molecules, together with the constructs enabling to express various membranous receptor molecules reported in Drosophila. We are trying to survey receptor molecules to reveal the Frizzled-1-ligand in question.

Research Products

• [Journal Article] Rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity. (2011)
  • Author(s): Zhang X-J, Li C, Gao H-L, Nabeka H, Shimokawa T, Wakisaka H, Matsuda S, Kobayashi N
  • Journal Title: Cellular and Molecular Biology Letters 162 Pages: 279-295
  • Peer Reviewed
• [Journal Article] Rho kinase inhibitors stimulate the migration of human cultured osteoblastic cells by regulating actomyosin activity. (2011)
  • Author(s): Zhang, X-J., Li, C., Gao, H-L., Nabeka, H., Shimokawa, T., Wakisaka, H., Matsuda, S., Kobayashi, N.
  • Journal Title: Cellular and Molecular Biology Letters Volume: 162 Pages: 279-295
  • Peer Reviewed
• [Journal Article] Sunspot, a link between Wingless signaling and endoreplication in Drosophila. (2010)
  • Author(s): Taniue K, Nishida A, Hamada F , Sugie A, Oda T, Ui-Tei K, Tabata T, Akiyama T
  • Journal Title: Development 137 Pages: 1755-1764
  • Peer Reviewed
• [Journal Article] Morphological maturation level of the esophagus is associated with the number of circumesophageal muscle fibers during archenteron formation in the starfish Patiria (Asterina) pectinifera. (2010)
  • Author(s): Miguchi Y, Takata H, Doihara T, Miyawaki K, Shimokawa T, Hamada F, Kobayashi N, Matsuda S
  • Journal Title: Biological Bulletin 219 Pages: 12-16
  • Peer Reviewed
• [Journal Article] Morphological maturation level of the esophagus is associated with the number of circumesophageal muscle fibers during archenteron formation in the starfish Patiria (Asterina) pectinifera (2010)
  • Author(s): Miguchi, Y., Takata, H., Doihara, T., Miyawaki, K., Shimokawa, T., Hamada, F., Kobayashi, N., Matsuda, S.
  • Journal Title: Biological Bulletin Volume: 219 Pages: 12-16
  • Peer Reviewed
• [Journal Article] Sunspot, a link between Wingless signaling and endoreplication in Drosophila (2010)
  • Author(s): Taniue, K., Nishida, A., Hamada, F., Sugie, A., Oda, T., Ui-Tei, K., Tabata, T., Akiyama, T.
  • Journal Title: Development Volume: 137 Pages: 1755-1764
  • Peer Reviewed
• [Journal Article] Spatiotemporal distribution patterns of oligosaccharides during early embryogen esis in the starfish Patiria pectinifera. (2009)
  • Author(s): Doihara T, Miguchi Y, Miyawaki K, Shimokawa T, Hamada F, Kobayashi N, Matsuda S.
  • Journal Title: Development Genes Evolution 219 Pages: 199-206
  • Peer Reviewed
• [Journal Article] Spatiotemporal distribution patterns of oligosaccharides during early embryogenesis in the starfish Patiria pectinifera (2009)
  • Author(s): Doihara T, Miguchi Y, Miyawaki K, Shimokawa T, Hamada F, Kobayashi N, Matsuda S
  • Journal Title: Development Genes Evolution 219 Pages: 199-206
  • Peer Reviewed
• [Journal Article] Trabid, a new positive regulator of Wnt-induced transcription with preference for binding and cleaving K63-linked ubiquitin chains. (2008)
  • Author(s): Tran H, Hamada F, Schwarz-Romond T, Bienz M
  • Journal Title: Genes and Development 22 Pages: 528-542
  • Peer Reviewed
• [Journal Article] Increase of integrin- linked kinase activity in cultured podocytes upon stimulation with plasma from patients with recurrent FSGS. (2008)
  • Author(s): Hattori M, Akioka Y, Chikamoto H, Kobayashi N, Tsuchiya K, Shimizu M, Kagami S, Tsukaguchi H
  • Journal Title: American Journal of Transplantation 8 Pages: 1550-1556
  • Peer Reviewed
• [Journal Article] Expression patterns in alternative splicing forms of prosaposin mRNA in the rat facial nerve nucleus after facial nerve transection. (2008)
  • Author(s): Chen J, Saito S, Kobayashi N, Sato K, Terashita T, Shimokawa T, Mominoki K, Miyawaki K, Sano A, Matsuda S
  • Journal Title: Neuroscience Research 60 Pages: 82-94
  • Peer Reviewed
• [Journal Article] Trabid, a new positive regulator of Wnt-induced transcription with preference for binding and cleaving K63-linked ubiquitin chains. (2008)
  • Author(s): Tran, H., Hamada, F., Schwarz-Romond, T., Bienz, M.
  • Journal Title: Genes and Development 22 Pages: 528-542
  • Peer Reviewed
• [Journal Article] Increase of integrin-linked kinase activity in cultured podocytes uponstimulation with plasma from patients with recurrent FSGS. (2008)
  • Author(s): Hattori, M., Kobayashi. N., 他(8名中4人目)
  • Journal Title: American Journal of Transplantation 8 Pages: 1550-1556
  • Peer Reviewed
• [Journal Article] Expression patterns in alternative splicing forms of prosaposin mRNA in the rat facial nerve nucleus after facial nerve transection. (2008)
  • Author(s): Chen, J., Kobayashi, N., 他(10名中3人目)
  • Journal Title: Neuroscience Research 60 Pages: 82-94
  • Peer Reviewed
• [Journal Article] カイニン酸神経細胞障害によるプロサポシン動態 (2008)
  • Author(s): 武智浩子, 上松敬吾, 濱田文彦, 下川哲哉, 小林直人, 松田正司
  • Journal Title: 愛媛医学 27 Pages: 120-126
  • Peer Reviewed
• [Presentation] Wntシグナル伝達経路を制御する新規ubiquitin ligaseの機能解析 (2009)
  • Author(s): 濱田文彦、鍋加浩明、H.Tran, M.Bienz, 小林直人、松田正司
  • Organizer: 日本解剖学会・第65回九州支部学術集会
  • Place of Presentation: 那覇
  • Year and Date: 2009-11-07
• [Presentation] Wntシグナル伝達経路を制御する新規 ubiquitin ligase の機能解析 (2009)
  • Author(s): 濱田文彦, 鍋加浩明, H.Tran, M.Bienz, 小林直人, 松田正司
  • Organizer: 日本解剖学会・第65回九州支部学術集会
  • Place of Presentation: 那覇
  • Year and Date: 2009-11-07
• [Presentation] Wntシグナル伝達経路を制御する新規ubiquitin ligaseの機能解析 (2009)
  • Author(s): 鍋加浩明、濱田文彦、宮脇恭史、下川哲哉、小林直人、松田正司
  • Organizer: 日本解剖学会・全国学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2009-03-30
• [Presentation] Wntシグナル伝達経路を制御する新規ubiquitin ligaseの機能解析 (2008)
  • Author(s): 濱田文彦、鍋加浩明、H.Tran, M.Bienz, 小林直人、松田正司
  • Organizer: 日本解剖学会総会・全国集会
  • Place of Presentation: 大分
  • Year and Date: 2008-03-28
• [Book] Dendritic Spines : Biochemistry, Modeling and Properties "The Structure of Dendritic Spines" (2009)
  • Author(s): Matsuda, Hamada, F, Ishizuka
  • Total Pages: 12
  • Publisher: NOVA Science publisher
• [Book] 細胞・培地活用ハンドブック 「32章 昆虫細胞」 (2008)
  • Author(s): 濱田文彦(分担執筆), 秋山徹, 河府和義編
  • Publisher: 羊土社
• [Remarks] ホームページ等
  • URL: http://kenqweb.office.ehime-u.ac.jp/Profiles/0008/0000359/profile.html
• [Remarks]
  • URL: http://kenqweb.office.ehime-u.ac.jp/Profiles/0008/0000359/profile.html