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New synthetic peptide enhances plasminogen activation and thrombolysis

Research Project

Project/Area Number 20590222
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionKinki University

Principal Investigator

OKADA Kiyotaka  Kinki University, 医学部, 講師 (20185432)

Co-Investigator(Kenkyū-buntansha) MATSUO Osamu  近畿大学, 医学部, 教授 (40030879)
KAWAO Naoyuki  近畿大学, 医学部, 助教 (70388510)
UESHIMA Shigeru  近畿大学, 農学部, 教授 (30193791)
NAGAI Nobuo  長浜バイオ大学, バイオサイエンス学部, 教授 (90260281)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords血液凝固 / 血液レオロジー / ペプチド / プラスミノーゲン / 線溶系 / プラスミノーゲンアクチベ / 血栓溶解 / マウス / スタヒロキナーゼ
Research Abstract

A new synthetic nonadecapeptide (SP) corresponding to Glu22-Leu40 of staphylokinase molecule bound to plasminogen (Plg) and enhanced the activation of Plg by Plg activator (PA). This binding was completely inhibited by synthetic peptide corresponding to C-terminal region of plasmin B-chain. SP binds to Glu-Plg, and induces structural changes of Glu-Plg. When SP was administrated in mouse thrombosis model, earlier recanalization was observed than in mice with vehicle administration. Thus, SP enhanced Plg activation and induced effective thrombolysis.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (21 results)

All 2011 2010 2009 2008

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (6 results)

  • [Journal Article] A synthetic peptide derived from staphylokinase enhances plasminogen activator by tissue-type plasminogen activator.2011

    • Author(s)
      Okada K, Ueshima S, Matsuno H, Nagai N, Kawao N, Tanaka M, Matsuo O
    • Journal Title

      J Thromb Haemost (Epub ahead of print.)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plasminogen/plasmin modulates bone metabolism by regulating the osteoblast and osteoclast function.2011

    • Author(s)
      Kanno Y, Ishisaki A, Kawashita E, Chosa, N, Nakajima K, Nishihara T, Toyoshima K, Okada K, Ueshima S, Matsushita K, Matsuo O, Matsuno H
    • Journal Title

      J Biol Chem

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Kawao N, Nagai N, Tamura Y, Okada K, Yano M, Suzuki Y, Umemura K, Ueshima S, Matsuo O2011

    • Author(s)
      Epub ahead of print.
    • Journal Title

      Urokinase-type plasminogen activator contributes to heterogeneity of macrophages at the border of damaged site during liver repair in mice. Thromb Haemost(Epub ahead of print.)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Localization of plasminogen in mouse hippocampus, cerebral cortex, and hypothalamus.2011

    • Author(s)
      Taniguchi Y, Inoue N, Morita S, Nikaido Y, Nakashima T, Nagai N, Okada K, Matsuo O, Miyata S
    • Journal Title

      Cell Tissue Res 343

      Pages: 303-317

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A synthetic peptide derived from staphylokinase enhances plasminogen activation by tissue-type plasminogen activator.2011

    • Author(s)
      Kiyotaka Okada
    • Journal Title

      Journal of Thrombosis and Haemostasis

      Volume: (Epub ahead of print)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of plasminogen in macrophage accumulation during liver repair.2010

    • Author(s)
      Kawao N, Nagai N, Okada K, Okumoto K, Ueshima S, Matsuo O
    • Journal Title

      Thromb Res 125

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plasminogen is essential for granulation tissue formation during the recovery process after liver injury in mice.2010

    • Author(s)
      Kawao N, Nagai N, Ishida C, Okada K, Okumoto K, Suzuki Y, Umemura K, Ueshima S, Matsuo O
    • Journal Title

      J Thromb Haemost 8

      Pages: 1555-1566

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Determination of factor VIII-interactive region within plasmin responsible for plasmin-catalyzed activation and inactivation of factor VIII(a).2010

    • Author(s)
      Nishiya K, Nogami R, Okada K, Matsuo O, Take M, Ogi K, Shima M
    • Journal Title

      Thromb Haemost 104

      Pages: 105-117

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of plasminogen in macrophage accumulation during liver repair2010

    • Author(s)
      Kawao N
    • Journal Title

      Thrombolysis Research Web

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of fibrinolytic activity in vascular endothelial cells by heterologous expression of adenine nucleotide translocase-12010

    • Author(s)
      Ishida C
    • Journal Title

      Blood Coagulation and Fibrinolysis Web

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alpha2-antiplasmin is associated with the progression of fibrosis2009

    • Author(s)
      Kanno Y
    • Journal Title

      American Journal of Pathology 176

      Pages: 238-245

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Binding of plasminogen to hepatocytes isolated from injured mice liver and nonparenchymal cell-dependent proliferation of hepatocytes.2008

    • Author(s)
      Okada K, Ueshima S, Kawao N, Okamoto C, Matsuo K, Akao M, Seki T, Ariga T, Tanaka M, Matsuo O.
    • Journal Title

      Blood Coagul Fibrin 19

      Pages: 503-511

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Binding of plasminogen to hepatocytes isolated from injured mouse liver and nonparenchymal-cell-dependent proliferation of hepatocytes.2008

    • Author(s)
      Okada K
    • Journal Title

      Blood Coagulation and Fibrinolysis 19

      Pages: 503-511

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The absence of uPAR is associated with the progression of dermal fibrosis.2008

    • Author(s)
      Kanno Y
    • Journal Title

      Journal of Investigative Dermatology 123

      Pages: 2792-2797

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alpha2-antiplasmin is a critical regulator of angiotensin II-madiated vascular remodeling.2008

    • Author(s)
      Hou Y
    • Journal Title

      Arterioscler Thrombosis Vascular Biology 28

      Pages: 1257-1262

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Syntetic noneecapeptide(SAK22-40)enhances plasminogen activation and thrombolysis.2010

    • Author(s)
      Kiyotaka Okada
    • Organizer
      20th International Congress on Fibrinolysis and Proteolysis
    • Place of Presentation
      Amsteraam, The Netherlands
    • Year and Date
      2010-08-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Synthetic nonadecapeptide (SAK22-40) enhances plasminogen activation and thrombolysis.2010

    • Author(s)
      Okada K
    • Organizer
      20th Internationl Congress on Fibrinolysis and Proteolysis.
    • Place of Presentation
      Amsterdam, The Netherlands.
    • Year and Date
      2010-08-24
    • Related Report
      2010 Final Research Report
  • [Presentation] 新規ペプチドの線溶系活性化促進作用の解析2010

    • Author(s)
      岡田清孝
    • Organizer
      第33回日本血栓止血学会学術集
    • Place of Presentation
      鹿児島
    • Year and Date
      2010-04-24
    • Related Report
      2010 Final Research Report
  • [Presentation] アンギオテンシンII誘発性の血管障害に対するα2-antiplasminの関与2009

    • Author(s)
      岡田清孝
    • Organizer
      第32回日本血栓止血学会学術集会
    • Place of Presentation
      小倉
    • Year and Date
      2009-06-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] 新規ペプチドのプラスミノーゲン活性化促進機序の解析2008

    • Author(s)
      岡田清孝
    • Organizer
      第31回日本血栓止血学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2008-11-22
    • Related Report
      2010 Final Research Report
  • [Presentation] 新規ペプチドのプラスミノーゲン活性化促進機序の解析2008

    • Author(s)
      岡田清孝
    • Organizer
      第31回日本血栓止血学会学術集会
    • Place of Presentation
      大阪市
    • Year and Date
      2008-11-22
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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