Molecular basis of the rhythmical expression of the genes in the gastrointestinal tract

• Project/Area Number: 20590233
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: University of Shizuoka
• Principal Investigator: GODA Toshinao University of Shizuoka, 食品栄養科学部, 教授 (70195923)
• Co-Investigator(Kenkyū-buntansha): MOCHIZUKI Kazuki 静岡県立大学, 食品栄養科学部, 助教 (80423838)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 時計遺伝子 / 小腸 / 日内リズム / 転写調節 / マイクロアレイ / 糖輸送担体

Research Abstract

Restricted feeding schedule led to a shift of the peak of the expression of clock gene (per 2) and a hexose transporter gene (sglt1) to just prior to the start of feeding. The binding of BMAL1 and acetylated histone H3 and H4 to the transcribed regions of these genes was associated with changes in their expressions. These results suggest that diurnal changes in expression of hexose transporter genes are regulated by a feedback loop of clock genes.

Research Products

• [Journal Article] Clock genes regulate the feeding schedule-dependent diurnal rhythm changes in hexose transporter gene expressions through the binding of BMAL1 to the promoter/enhancer and transcribed regions. (2011)
  • Author(s): Iwashina, I., Mochizuki, K., Inamochi, Y., Goda, T.
  • Journal Title: J.Nutr.Biochem. Vol.22 Pages: 334-343
• [Journal Article] Diet-induced epigenetic regulation in vivo of the intestinal fructose transporter GLUT5 during development of rat small intestine. (2011)
  • Author(s): Suzuki, T., ら
  • Journal Title: Biochem.J. Volume: 435 Pages: 43-53
  • Peer Reviewed
• [Journal Article] Feeding rats dietary resistant starch reduces both the binding of ChREBP and the acetylation of histones on the Thrsp gene in the jejunum. (2011)
  • Author(s): Shimada, M.
  • Journal Title: J.Agr.Food Chem. Volume: 59 Pages: 1464-1469
  • Peer Reviewed
• [Journal Article] Jejunal induction of SI and SGLT1 genes in rats by high-starch/low-fat diet is associated with histone acetylation and binding of GCN5 on the genes. (2011)
  • Author(s): Inoue, S., ら
  • Journal Title: J.Nutr.Sci.Vitaminol. Volume: 57 Pages: 162-169
  • Peer Reviewed
• [Journal Article] Histone H3 methylation at lysine 4 on the SLC2A5 gene in intestinal Caco-2 cells is involved in SLC2A5 expression. (2010)
  • Author(s): Inamochi, Y., Mochizuki, K., Osaki, A., Ishii, T., Nakayama, T., Goda, T.
  • Journal Title: Biochem.Biophys.Res.Commu. Vol.392 Pages: 16-21
• [Journal Article] Histone H3 methylation at lysine 4 on the SLC2A5 gene in intestinal Caco-2 cells is involved in SLC2A5 expression (2010)
  • Author(s): Inamochi, Y., ら
  • Journal Title: Biochem.Biophys.Res.Commu. 392 Pages: 16-21
  • Peer Reviewed
• [Journal Article] The regulation of jejunal induction of the maltase-glucoamylase gene by a high-starch/low-fat diet in mice (2010)
  • Author(s): Mochizuki, K., ら
  • Journal Title: Mol.Nutr.Food Res. 54 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Clock genes regulate the feeding schedule-dependent diurnal rhythm changes in hexose transporter gene expressions through the binding of BMAL1 to the promoter/enhancer and transcribed regions (2010)
  • Author(s): Iwashina, I., ら
  • Journal Title: J.Nutr.Biochem. (印刷中)
  • Peer Reviewed
• [Journal Article] Induction of histone acetylation on the sucrase-isomaltase gene in the postnatal rat jejunum (2009)
  • Author(s): Yorita, S., ら
  • Journal Title: Biosci.Biotechnol.Biochem. 73 Pages: 933-935
  • Peer Reviewed
• [Journal Article] Gene expression changes in the jejunum of rats during the transient suckling-weaning period (2009)
  • Author(s): Mochizuki, K., ら
  • Journal Title: J.Nutr.Sci.Vitaminol. 55 Pages: 139-148
  • NAID: 10025970814
  • Peer Reviewed
• [Journal Article] Localized expression of genes related to carbohydrate and lipid absorption along the crypt-villus axis of rat jejunum (2009)
  • Author(s): Suzuki, T., ら
  • Journal Title: Biochim.Biophys.Acta 1790 Pages: 1624-1635
  • Peer Reviewed
• [Journal Article] Feeding rats dietary resistant starch shifts the peak of SGLT1 gene expression and histone H3 acetylation on the gene from the upper jejunum towards the ileum (2009)
  • Author(s): Shimada, M., ら
  • Journal Title: J.Agr.Food Chem. 57 Pages: 8049-8055
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 合田敏尚
  • Journal Title: 臨床栄養医学(南山堂) Pages: 96-101
• [Journal Article] Inductions of histone H3 acetylation at lysine 9 on SGLT1 gene and its expression by feeding mice a high carbohydrate/fat ratio diet. (2009)
  • Author(s): Honma, K.
  • Journal Title: Nutrition 25 Pages: 40-44
  • Peer Reviewed
• [Journal Article] Inductions of histone H3 acetylation at lysine 9 on SGLT1 gene and its expression by feeding mice a high carbohydrate/fat ratio diet. (2008)
  • Author(s): Honma, K., Mochizuki, K., Goda, T.
  • Journal Title: Nutrition Vol.25 Pages: 40-44
• [Journal Article] Dietary resistant starch reduces levels of GIP mRNA along the jejunum-ileum in both normal and type 2 diabetic rats. (2008)
  • Author(s): Shimada, M.
  • Journal Title: Biosci. Biotech. Biochem. 72 Pages: 2206-2209
  • Peer Reviewed
• [Journal Article] Higher expression of jejunal LPH gene in rats fed the high-carbohydrate/low-fat diet compared with in those fed the lowcarbohydrate/high-fat diet is associated with in vitro binding of Cdx-2 in nuclear proteins to its promoter regions. (2008)
  • Author(s): Tanaka, T.
  • Journal Title: Life Sciences 83 Pages: 122-127
  • Peer Reviewed
• [Presentation] ヒストンコードとmRNA伸長因子を介した小腸消化吸収関連遺伝子発現の日内リズム発振機構 (2010)
  • Author(s): 山内浩之、望月和樹、合田敏尚
  • Organizer: 第64回日本栄養・食糧学会大会
  • Place of Presentation: 徳島
  • Year and Date: 2010-05-23
• [Presentation] 小腸様細胞株Caco-2の分化に伴うGLUT5遺伝子発現増大におけるヒストンH3K4のメチル化の役割 (2010)
  • Author(s): 稲用裕子, 他
  • Organizer: 第64回日本栄養・食糧学会大会
  • Place of Presentation: 徳島
  • Year and Date: 2010-05-23
• [Presentation] 糖質摂取時に増大する小腸糖質消化吸収関連遺伝子発現のヒストンテール翻訳後修飾を介した調節機構 (2010)
  • Author(s): 井上聖哉, 他
  • Organizer: 第64回日本栄養・食糧学会大会
  • Place of Presentation: 徳島
  • Year and Date: 2010-05-22
• [Presentation] Ontogenetic changes of histone modifications and polymerase II binding in the promoter region of rat intestinal sugar transporter genes in vivo. (2010)
  • Author(s): 鈴木拓史, 他
  • Organizer: Experimental Biology 2010
  • Place of Presentation: Anaheim(USA)
  • Year and Date: 2010-04-27
• [Presentation] Regulation of intestinal carbohydrate digestion/absorption-related genes : Mechanisms and Applications (2009)
  • Author(s): 合田敏尚
  • Organizer: 15^<th> Korea-Japan Symposium on Diabetes Mellitus
  • Place of Presentation: Jeju(Korea)
  • Year and Date: 2009-11-20
• [Presentation] マウス脂肪組織におけるヒストンコードを介した脂肪合成関連遺伝子発現の日内リズム (2009)
  • Author(s): 五十嵐信人、望月和樹、合田敏尚
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] 遺伝子発現の周期性維持に関与する転写産物の分子生物学的ならびに統計学的手法を用いた探索法の開発 (2009)
  • Author(s): 望月和樹
  • Organizer: 2008 USフォーラム
  • Place of Presentation: 静岡
  • Year and Date: 2009-08-05
• [Presentation] 小腸様細胞Caco-2におけるGLUT5発現誘導におけるグルココルチコイドホルモン受容体のリン酸化修飾の役割 (2009)
  • Author(s): 稲用裕子, 他
  • Organizer: 第63回日本栄養・食糧学会大会
  • Place of Presentation: 長崎
  • Year and Date: 2009-05-21
• [Presentation] クロマチンリモデリング因子による小腸吸収細胞遺伝子発現誘導機構 (2009)
  • Author(s): 望月和樹
  • Organizer: 第63回日本栄養・食糧学会大会
  • Place of Presentation: 長崎
  • Year and Date: 2009-05-20
• [Presentation] Clock genes regulate circadian rhythmical change of sglt1 gene expression according to feeding schedule through binding of BMAL1 on the promoter region. (2009)
  • Author(s): Goda, T., Iwashina, I., Mochizuki K.
  • Organizer: Experimental Biology 2009
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2009-04-20
• [Presentation] Clock genes regulate circadian rhythmical change of sglt1 gene expression according to feeding schedule through binding of BMAL1 on the promoter region (2009)
  • Author(s): 合田敏尚, 他
  • Organizer: Experimental Biology 2009
  • Place of Presentation: New Orleans(USA)
  • Year and Date: 2009-04-20
• [Presentation] 小腸由来幹細胞様細胞株IEC-6細胞の分化型形質獲得をもたらす転写因子とホルモンの相互作用 (2008)
  • Author(s): 鈴木拓史, 他
  • Organizer: 第62回日本栄養・食糧学会大会
  • Place of Presentation: 坂戸
• [Presentation] 時計遺伝子による小腸消化吸収関連遺伝子の発現調節 (2008)
  • Author(s): 合田敏尚
  • Organizer: 2007 USフォーラム
  • Place of Presentation: 静岡
• [Presentation] 糖輸送祖体遺伝子のヒストンコードを介した転写調節 (2008)
  • Author(s): 合田敏尚
  • Organizer: 第39回日本消化吸収学会総会
  • Place of Presentation: 東京
• [Presentation] 難消化性糖質による糖質の消化・吸収遅延作用とその応用 (2008)
  • Author(s): 合田敏尚
  • Organizer: 第13回日本食物繊維学会学術集会
  • Place of Presentation: 東京
• [Presentation] 小腸細胞分化・成熟関連転写因子の安定的発現に伴う細胞周期への影響 (2008)
  • Author(s): 鈴木拓史, 他
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸
• [Book] 臨床栄養医学 (2009)
  • Author(s): 合田敏尚
  • Publisher: 南山堂
• [Book] 新時代の糖尿病学(I) -病因・診断・治療研究の進歩- (2008)
  • Author(s): 合田敏尚
  • Publisher: 日本臨床
• [Remarks]
  • URL: http://sfns.u-shizuoka-ken.ac.jp/nutrphys/
• [Remarks]
  • URL: http://sfns.u-shizuoka-ken.ac.jp/nutrphys/
• [Remarks]
  • URL: http://sfns.u-shizuoka-ken.ac.jp/nutrphys/