Studies on the roles for 8-nitroguanosine 3',5'-cyclic monophosphate in the cardiovascular system and the applications to drug discovery.
| Project/Area Number |
20590255
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Kumamoto University |
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Principal Investigator |
TOKUTOMI Yoshiko Kumamoto University, 生活科学部, 教授 (90253723)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | cGMP / eNOS / 心血管系 / NO / 一酸化窒素 / db |
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| Research Abstract |
8-Nitroguanosine 3',5'-cyclic monophosphate (8-nitro-cGMP), formed nitric oxide (NO)-dependently, is a novel physiological second messenger. To study the physiological activity of 8-nitro-cGMP in the cardiovascular system, we studied its effects on vascular reactivity of mouse aortas. 8-Nitro-cGMP induced enhancement of contraction to phenylephrine and relaxation concentration-dependently in aortas from non-diabetic mice, but only relaxation in aortas from diabetic mice. It was suggested that 8-nitrocGMP- induced superoxide production via eNOS uncoupling may mediate the enhancement of the phenylephrine contraction. The vasodilator effect of 8-nitro-cGMP may contribute to amelioration of the vascular endothelial dysfunction in diabetic mice, representing a novel pharmacological approach toprevent the complications associated with diabetes.
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Report
(4 results)
Research Products
(29 results)
