Analysis of regulatory mechanism of motility essential for Entamoeba encystation and excystation

• Project/Area Number: 20590432
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Jikei University School of Medicine
• Principal Investigator: MAKIOKA Asao Jikei University School of Medicine, 医学部, 准教授 (90119850)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: Entamoeba / 嚢子形成 / 脱嚢 / コフィリン / プロフィリン / アクチン細胞骨格 / cytochalasin D / Arp2 / 3

Research Abstract

The encystation and excystation of Entamoeba are important processes for formation of the infective form and establishment of the infection. The present study focused on an actin depolymerizing factor cofilin and profilin, which are essential for reorganization of actin cytoskeleton, and investigated their identification, gene cloning, phylogeny, preparation of recombinant proteins, localization and expression analysis and found different expression of each molecules.

Research Products

• [Journal Article] Entamoeba invadens : identification of ADF/cofilin and their expression analysis in relation to encystation and excystation. (2011)
  • Author(s): Makioka A, Kumagai M, Hiranuka K, Kobayashi S, Takeuchi T
  • Journal Title: Exp Parasitol 127 Pages: 195-201
  • Peer Reviewed
• [Journal Article] Entamoeba invadens : identification of ADF/cofilin and their expression analysis in the encystation and excystation (2011)
  • Author(s): Makioka A
  • Journal Title: Exp Parasitol Volume: 127 Pages: 195-201
  • Peer Reviewed
• [Journal Article] Involvement of serine proteases in the excystation and metacystic development of Entamoeba invadens (2009)
  • Author(s): Makioka A
  • Journal Title: Parasitol Res 105 Pages: 977-987
  • Peer Reviewed
• [Presentation] Entamoebaの脱嚢に伴うアクチン脱重合因子コフィリンの発現解析 (2010)
  • Author(s): 牧岡朝夫
  • Organizer: 第79回日本寄生虫学会大会
  • Place of Presentation: 旭川
  • Year and Date: 2010-05-21
• [Presentation] Entamoebaの脱嚢に伴うアクチン脱重合因子コフィリンの発現解析 (2010)
  • Author(s): 牧岡朝夫
  • Organizer: 日本寄生虫学会
  • Place of Presentation: 大雪クリスタルホール(旭川市)
  • Year and Date: 2010-05-21
• [Presentation] Entamoebaのアクチン脱重合因子コフィリンの解析 (2009)
  • Author(s): 牧岡朝夫
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] Entamoebaのアクチン脱重合因子コフィリンの解析 (2009)
  • Author(s): 牧岡朝夫
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-12-11
• [Presentation] Entamoebaの脱嚢におけるコフィリンの発現解析 (2009)
  • Author(s): 牧岡朝夫
  • Organizer: 日本原生動物学会
  • Place of Presentation: 石巻専修大学(石巻市)
  • Year and Date: 2009-11-01
• [Presentation] Entamoebaの脱嚢におけるコフィリンの発現解析 (2009)
  • Author(s): 牧岡朝夫
  • Organizer: 第42回日本原生動物学会大会
  • Place of Presentation: 石巻
  • Year and Date: 2009-10-30