Development of the hypersensitive and rapid detection method for NF-κB activation using by Fluorescence Correlation Spectroscopy and its application to the emergency medicine
| Project/Area Number |
20590559
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | University of Toyama |
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Principal Investigator |
KITAJIMA Isao University of Toyama, 大学院・医学薬学研究部(医学), 教授 (50214797)
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| Co-Investigator(Kenkyū-buntansha) |
NIIMI Hideki 富山大学, 附属病院検査部, 副部長,助教 (50401865)
HAKAKEYAMA Noboru 富山大学, 附属病院手術部, 副部長,准教授 (70251907)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 転写因子 / NF-κB / 1分子蛍光相関法 / 全身炎症反応症候群 / 救急医療 / 1分子蛍光法 / 炎症性サイトカイン / 迅速検査 / 全身性炎症反応候群 / 菌血症 / 全身性炎症反応症候群 / 一分子蛍光法 / 核蛋白抽出法 |
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| Research Abstract |
We have established a novel measurement system for the NF-κB activity using fluorescence correlation spectroscopy (FCS), which was a methodology to examine the size and number of fluorescence-labeled molecules in a confocal area by direct observation of their Brownian motion in solutions. The principle of the measurement was to detect the different of molecular weights of fluorescent-labeled DNA probe and DNA probe-NF-κB complex. The elevation of NF-κB activity reflects the acute inflammation. Measuring the NF-κB activity in the patients with systemic inflammatory response syndrome (SIRS) would be useful as an inflammatory marker.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Reduced expression of the ATRX gene, a chromatin-remodeling factor, causes hippocampal dysfunction in mice.2010
Author(s)
Nogami T, Beppu H, Tokoro T, Moriguchi S, Shioda N, Fukunaga K, Ohtsuka T, Ishii Y, Sasahara M, Shimada Y, Nishijo H, Li E, Kitajima I
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Related Report
Peer Reviewed
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[Journal Article] Modulation of glucocorticoid receptor expression, inflammation and cell apoptosis in septic guinea-pig lung using methylprednisolone.2008
Author(s)
Kamiyama K, Matsuda N, Yamamoto S, Takano K, Takano Y, Yamazaki H, Kageyama SI, Kokoo H, Nagata H, Hatakeyama N, Tsukada K, Hattori Y
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Journal Title
Am J Physiol Lung Cell Mol Physiol. 259
Related Report
Peer Reviewed
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