Novel method of measuring activities of enzymes involved in lipid metabolism
| Project/Area Number |
20590560
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kanazawa University |
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Principal Investigator |
KOBAYASHI Junji Kanazawa University, 医学系研究科, 特任教授 (60302577)
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| Co-Investigator(Kenkyū-buntansha) |
NOHARA Atsushi 金沢大学, 医学系研究科, 特任助教 (50313648)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | LPL / HTGL / ANGPTL3 / 肝性リパーゼ / 内臓脂肪 / インスリン抵抗性 / リポ蛋白リパーゼ / sdLDL / メタボリックシンドローム / 低HDL |
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| Research Abstract |
We have established a simple and reliable system for measuring LPL and HTGL activities in post-heparin plasma and investigated clinical significance of measuring those activities. LPL activities showed inverse relations with TG, RLP-TG and sdLDL and positive relations with HDL-C but had no relation with ANGPTL3. HTGL activities showed no relation with TG, RLP-TG and sdLDL but had inverse relations with HDL-C and ANGPTL3. In vitro experiment showed that ANGPTL3 did not inhibit HTGL activities at all, suggesting unidentified molecule may regulate ANGPTL3 and HTGL each other in opposite directions.
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Report
(4 results)
Research Products
(37 results)
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[Journal Article] Expression of human apoAII in transgenic rabbits leads to dyslipidemia. A new model for combined hyperlipidemia.2009
Author(s)
Koike T, Kitajima S, Yu Y, Li Y, Nishijima K, Liu E, Sun H, Waqar AB, Shibata N, Inoue T, Wang Y, Zhang B, Kobayashi J, Morimoto M, Saku K, Watanabe T, Fan J.
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Journal Title
Arterioscler Thromb Vasc Biol 29
Pages: 2047-2053
Related Report
Peer Reviewed
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[Journal Article] A novel method for measuring human lipoprotein lipase and hepatic lipase activities in post-heparin plasma.2008
Author(s)
Imamura S, Kobayashi J, Nakajima K, Sakasegawa S, Nohara A, Noguchi T, Kawashiri M, Inazu A, Deeb SS, Mabuchi H, Brunzell JD
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Journal Title
Journal of Lipid Research 49
Pages: 1431-1437
Related Report
Peer Reviewed
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