Proteomic investigation of oxidized proteins in cerebrospinal fluid for developing novel biomarkers of Alzheimer's disease.

• Project/Area Number: 20590594
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology (2009-2012); Tokyo Metropolitan Institute of Gerontology (2008)
• Principal Investigator: TODA Tosifusa 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (80133635)
• Co-Investigator(Renkei-kenkyūsha): NAKAMURA Magumi 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究助手 (10159072) ; SHIBASAKI Futoshi 財団法人東京都医学研究機構, 東京都臨床医学総合研究所, 研究員 (90300954)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2012: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 病態検査医学 / バイオマーカー / 神経変性疾患 / 髄液マーカー / 酸化タンパク質 / 認知症 / 髄液バイオマーカー / プロテオーム解析 / 臨床検査医学 / 軽度認知障害 / カルボニル化タンパク質

Research Abstract

Proteomic analysis of oxidatively modified proteins was performed on spinal fluid of Alzheimer's disease patients. As a consequence of in-gel digestion and mass spectrometric identification, it has been clarified that transthyretin is preferentially oxidized in spinal fluid of Alzheimer's disease patients. Oxidative modification of transthyretin might be involved in incidence of Alzheimer's disease.

Research Products

• [Journal Article] Differential responses of white adipose tissue and brown adipose tissue to caloric restriction in rats. (2012)
  • Author(s): Okita N, Hayashida Y, Kojima Y, Fukushima M, Yuguchi K, Mikami K, Yamauchi A, Watanabe K, Noguchi M, Nakamura M, Toda T, Higami Y.
  • Journal Title: Mech Ageing Dev. Volume: 133(5) Issue: 5 Pages: 255-266
  • DOI: 10.1016/j.mad.2012.02.003
  • Peer Reviewed
• [Journal Article] Proteomic approaches to oxidative protein modifications implicated in the mechanism of aging (2012)
  • Author(s): Toda T, Nakamura M, Morisawa H, Hirota M, Nishigaki R, Yoshimi Y.
  • Journal Title: Geriatr Gerontol Int Volume: 10(Suppl.1)
  • NAID: 10027272777
• [Journal Article] Galectin-7 as a marker of cholesteatoma residue and its detection during surgery by an immunofluorescent methodva preliminary study (2012)
  • Author(s): 高木大樹
  • Journal Title: Ontology and Neurotology Volume: 33 Issue: 3 Pages: 396
  • DOI: 10.1097/mao.0b013e3182488021
  • Peer Reviewed
• [Journal Article] Proteomic identification of differentially expressed genes during differentiation of cynomolgus monkey (Macaca fascicularis) embryonic stem cells to astrocyte progenitor cells in vitro. (2012)
  • Author(s): 赤間邦子
  • Journal Title: Biochimimica et Biophysica Acta Volume: 1834 Issue: 2 Pages: 601-610
  • DOI: 10.1016/j.bbapap.2012.12.002
  • Peer Reviewed
• [Journal Article] Up-regulation of 42 kDa tubulin alpha-6 chain fragment in well-differentiated hepatocellular carcinoma tissues from patients infected with hepatitis C virus (2011)
  • Author(s): Kuramitsu Y, Takashima M, Yokoyama Y, Iizuka N, Tamesa T, Akada JK, Wang Y, Toda T, Sakaida I, Okita K, Oka M, Nakamura K.
  • Journal Title: Anticancer Res. Volume: 31(10) Pages: 3331-3336
  • Peer Reviewed
• [Journal Article] Morphological changes in diabetic kidney are associated with increased O-GlcNAcylation of cytoskeletal proteins including alpha-actinin 4 (2011)
  • Author(s): Akimoto Y, Miura Y, Toda T, Wolfert MA, Wells L, Boons G-J, Hart GW, Endo T, Kawakami H
  • Journal Title: Clin Proteomics. Volume: 8(15) Issue: 1 Pages: 1-16
  • DOI: 10.1186/1559-0275-8-15
  • Peer Reviewed
• [Journal Article] Quantitative evaluation of lectin-reactive glycoforms of alpha1-acid glycoprotein using lectin affinity capillary electrophoresis with fluorescence detection (2011)
  • Author(s): Shimura K, Tamura M, Toda T, Yazawa S, Kassai K
  • Journal Title: Electrophoresis Volume: 32(16) Issue: 16 Pages: 2188-2193
  • DOI: 10.1002/elps.201100146
  • Peer Reviewed
• [Journal Article] Proteomic analysis of two types of exosomes in human whole saliva (2011)
  • Author(s): Ogawa Y, Miura Y, Harazono A, Kanai-Azuma M, Akimoto Y, Kawakami H, Yamaguchi T, Toda T, Endo T, Tsubuki M., Yanoshita R.
  • Journal Title: Biol. Pharm. Bull. Volume: 34(1) Pages: 13-23
  • NAID: 130000402256
  • Peer Reviewed
• [Journal Article] proteomic identification of differentially expressed genes in neural stem cells and neurons differentiated from embryonic stem cells of cynomolgus monkey (Macaca fascicularis) in vitro (2011)
  • Author(s): Akama K, Horikoshi T, Nakayama T, Otsu M, Imaizumi N, Nakamura M, Toda T, Inuma M, Hirano H, Kondo Y, Suzuki Y, Inoue N.
  • Journal Title: Biochim Biophys Acta. Volume: 1814 Issue: 2 Pages: 265-276
  • DOI: 10.1016/j.bbapap.2010.10.009
  • Peer Reviewed
• [Journal Article] Proteomic analysis of plasma proteins in Japanese semisuper centenarians (2011)
  • Author(s): Miura Y, Sato Y, Arai Y, Abe Y, Takayama M, Toda T, Hirose N, Endo T.
  • Journal Title: Exp Gerontol. Volume: 46 Issue: 1 Pages: 81-85
  • DOI: 10.1016/j.exger.2010.10.002
  • Peer Reviewed
• [Journal Article] Morphological changes in diabetic kidney are associated with increased O-GlcNAcylation of cytoskeletal proteins including alpha-actinin 4 (2011)
  • Author(s): 秋元義弘
  • Journal Title: Clinical Proteomics Volume: 8 Pages: 1-16
  • Peer Reviewed
• [Journal Article] Proteomic analysis of plasma proteins in Japanese semisuper centenarians (2011)
  • Author(s): Miura Y, Sato Y, Arai Y, Abe Y, Takayama M, Toda T, Hirose N, Endo T.
  • Journal Title: Experimental Gerontology. 46 Pages: 81-85
  • Peer Reviewed
• [Journal Article] Proteomic analysis of plasma proteins in Japanese semisuper centenarians (2011)
  • Author(s): Miura Y, Sato Y, Arai Y, Abe Y, Takayama M, Toda T, Hirose N, Endo T
  • Journal Title: Experimental Gerontology Volume: 46 Pages: 801-885
  • Peer Reviewed
• [Journal Article] Protein disulfide isomerase-P5, down-regulated in the final stage of boar epididymal sperm maturation, catalyzes disulfide formation to inhibit protein function in oxidative refolding of reduced denatured lysozyme. (2010)
  • Author(s): Akama K, Horikoshi T, Sugiyama A, Nakahata S, Akitsu A, Niwa N, Intoh A, Kakui Y, Sugaya M, Takei K, Imaizumi N, Sato T, Matsumoto R, Iwahashi H, Kashiwabara SI, Baba T, Nakamura M, Toda T.
  • Journal Title: Biochim Biophys Acta. Volume: 1804(6) Issue: 6 Pages: 1272-1284
  • DOI: 10.1016/j.bbapap.2010.02.004
  • Peer Reviewed
• [Journal Article] Proteomic approaches to oxidative protein modifications implicated in the mechanism of aging (2010)
  • Author(s): Toda T, Nakamura M, Morisawa H, Hirota M, Nishigaki R, Yoshimi Y.
  • Journal Title: Geriatrics Gerontology International 10(Supplement 1)
  • NAID: 10027272777
  • Peer Reviewed
• [Journal Article] Protein disulfide isomerase-P5, down-regulated in the final stage of boar epididymal sperm maturation, catalyzes disulfide formation to inhibit protein function in oxidative refolding of reduced denatured lysozyme (2010)
  • Author(s): Akama K, Horikoshi T, Sugiyama A, Nakahata S, Akitsu A, Niwa N, Intoh A, Kakui Y, Sugaya M, Takei K, Imaizumi N, Sato T, Matsumoto R, Iwahashi H, Kashiwabara SI,Baba T, Nakamura M, Toda T.
  • Journal Title: Biochimica et Biophysics Acta Volume: 1804 Pages: 1272-1284
  • Peer Reviewed
• [Journal Article] Proteomic approaches to oxidative protein modifications implicated in the mechanism of aging (2010)
  • Author(s): Toda T, Nakamura M, Morisawa H, Hirota M, Nishigaki R, Yoshimi Y.
  • Journal Title: Geriatrics and Gerontology International Volume: 10(Supp1.1)
  • NAID: 10027272777
• [Journal Article] Proteomic analysis of protein complexes in human SH-SY5Y neuroblastoma cells by using blue-native gel electrophoresis : An increase in lamin A/C associated with heat shock protein 90 in response to 6-hydroxydopamine-induced oxidative stress (2009)
  • Author(s): Nakamura M, Morisawa H, Imajoh-Ohmi S, Takamura C, Fukuda H, Toda T.
  • Journal Title: Exp. Gerontol. Volume: 44(6-7) Pages: 375-382
  • URL: http://dx.doi.org/10.1016/j.exger.2009.02.009
  • Peer Reviewed
• [Journal Article] Glycoproteomic analysis of abnormal N-glycosylation on the kappa chain of cryocryst alglobulin in a patient of multiple myeloma (2009)
  • Author(s): Toda T, Nakamura M, Yamada M, Nishine T, Torii T, Ikenaka K, Mori M.
  • Journal Title: J. Electrophoresis Volume: 53 Pages: 1-6
  • NAID: 10025191936
  • URL: http://dx.doi.org/10.2198/jelectroph.53.7
  • Peer Reviewed
• [Journal Article] Proteomic analysis of protein complexes in human SH-SY5Y neuroblastoma cells by using blue-native gel electrophoresis : An increase in lamin A/C associated with heat shock protein 90 in response to 6-hydroxydopamine-induced oxidative stress (2009)
  • Author(s): Nakamura M, Morisawa H, Imajoh-Ohmi S, Takamura C, Fukuda H, Toda T
  • Journal Title: Experimental.Gerontology 44(6-7) Pages: 375-382
  • Peer Reviewed
• [Journal Article] Proteomic analysis of protein complexes in human SH-SY5Y neuroblastoma cells by using blue-native gel electrophoresis : An increase in lamin A/C associated with heat shock protein 90 in response to 6-hydroxydopamine-induced oxidative stress (2009)
  • Author(s): Nakamura M, Morisawa H, Imajoh-Ohmi S, Takamura C, Fukuda H, Toda T
  • Journal Title: Experimental Gerontology 44 Pages: 375-382
  • Peer Reviewed
• [Journal Article] Involvement of Galectin-3 with Vacular Cell Adhesion Molecule-1 in Growth Regulation of Mouse BALB/3T3 cells (2009)
  • Author(s): Tadokoro T, Ikekita M, Toda T, Ito H, Sato T, Nakatani R, Hamaguchi Y, Furukawa K
  • Journal Title: Journal of Biological Chemistry 284 Pages: 35556-35563
  • Peer Reviewed
• [Journal Article] ラミンA-その多彩な機能と老化- (2009)
  • Author(s): 中村愛、森澤拓、戸田年総
  • Journal Title: 基礎老化研究 33 Pages: 3-10
  • NAID: 10026103704
  • Peer Reviewed
• [Journal Article] Glycoproteomic analysis of abnormal N-glycosylation on the kappa chain of cryocryst alglobulin in a patient of multiple myeloma (2009)
  • Author(s): Toda T, Nakamura M, Yamada M, Nishine T, Torii T, Ikenaka K, Mori M.
  • Journal Title: J. Electrophoresis 53 Pages: 1-6
  • NAID: 10025191936
  • Peer Reviewed
• [Journal Article] Proteomic identification of differentially expressed genes in ouse neural stem cells and neurons differentiated from embryonic stem cells in vitro (2008)
  • Author(s): Akama K, Tatsuno R. Otsu M, Horikoshi T, Nakamura M, Toda T, Inoue N
  • Journal Title: Biochim. Biophys. Acta. 1784 Pages: 773-782
  • Peer Reviewed
• [Journal Article] Analysis of Glycopetides Using Lectin Affinity Chromatography with MALDI-TOF Mass Spectrometry (2008)
  • Author(s): Kubota K, Sato Y, Suzuki Y, Goto-Inoue N, Toda T, Suzuki M, Endo T.
  • Journal Title: Anal. Chem. 80 Pages: 3693-3698
  • Peer Reviewed
• [Presentation] 糖尿病性腎症に伴うタンパク質への糖修飾異常の解析 (2013)
  • Author(s): 秋元義弘
  • Organizer: 第118回日本解剖学会総会
  • Place of Presentation: 高松
• [Presentation] 質量分析データリポジトリの開発 (2012)
  • Author(s): 森 澤拓、廣田三佳子、岩本真知子、三浦ゆり、戸田年総
  • Organizer: 第10回JHUPO日本プロテオーム学会
  • Place of Presentation: 東京
• [Presentation] Proteomic identification of differentially expressed genes during differentiation of cynomolgus monkey embryonic stem cells to astrocyte progenitor cells in vitro (2012)
  • Author(s): Kuniko Akama1, Tomoe Horikoshi, Takashi Nakayama, Masahiro Otsu, Noriaki Imaizumi, Megumi Nakamura, Tosifusa Toda, Michiko Inuma, Hisashi Hirano, Yasushi Kondo, Yutaka Suzuki, Nobuo Inoue
  • Organizer: HUPO 11th Annual World Congress
  • Place of Presentation: ボストン、米国
• [Presentation] 糖尿病角膜症に伴うタンパク質への糖(O-GlcNAc)修飾の変化 (2012)
  • Author(s): 秋元義弘
  • Organizer: 日本顕微鏡学会第68回学術講演会
  • Place of Presentation: つくば
• [Presentation] Detection of O-GlcNAcylated Proteins by Glycoproteomics and in situ Proximity Ligation Assay (PLA) (2012)
  • Author(s): 秋元義弘
  • Organizer: 第14回国際組織細胞化学会議
  • Place of Presentation: 京都
• [Presentation] Proteomic identification of differentially expressed genes during differentiation of cynomolgus monkey embryonic stem cells to astrocyte progenitor cells in vitro (2012)
  • Author(s): 赤間邦子
  • Organizer: HUPO 11th Annual World Congress
  • Place of Presentation: ボストン
• [Presentation] プロテオーム解析による健康長寿マーカーの探索---日本人超百寿者血漿の網羅的解析--- (2011)
  • Author(s): 三浦ゆり、新井康通、戸田年総、広瀬信義、遠藤玉夫
  • Organizer: 日本基礎老化学会第34回大会
  • Place of Presentation: 東京
• [Presentation] プロテオミクス用研究情報管理システムにおける電気泳動画像データ、質量分析データの表示、再解析機能の開発 (2011)
  • Author(s): 森澤 拓、廣田三佳子、岩本真知子、戸田年総
  • Organizer: 日本ヒトプロテオーム機構第9回大会(日本プロテオーム学会2011年会)
  • Place of Presentation: 新潟
• [Presentation] 日本人超百寿者血漿タンパク質のプロテオミクス (2011)
  • Author(s): 三浦ゆり、新井康通、戸田年総、広瀬 信義、遠藤 玉夫
  • Organizer: 日本ヒトプロテオーム機構第9回大会(日本プロテオーム学会2011年会)
  • Place of Presentation: 新潟
• [Presentation] メチオニンスルホキシドペプチドを標的としたフォーカストプロテオミクスによる細胞内酸化ストレス動態の網羅的解析 (2011)
  • Author(s): 戸田年総、岩本真知子、森澤拓、中家修一、尾島典行
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] 二次元電気泳動分離スポットの質量分析による酸化メチオニンレベルの定量～タンパク質酸化修飾指標としての有用性について～ (2011)
  • Author(s): 戸田年総、岩本真知子、森澤拓、中家修一、尾島典行
  • Organizer: 第62回日本電気泳動学会
  • Place of Presentation: 横浜
• [Presentation] 新規細胞死誘導剤リダイフェン-Gの薬物標的分子の同定 (2011)
  • Author(s): 羽鳥麻奈美
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] 全自動二次元電気泳動装置を使用した14-3-3タンパク質アイソフォームの解析 (2011)
  • Author(s): 岩本真知子
  • Organizer: 第62回日本電気泳動学会
  • Place of Presentation: 横浜
• [Presentation] プロテオーム用研究情報管理システムの拡張-再解析機能ツールの開発- (2011)
  • Author(s): 森澤拓
  • Organizer: 第62回日本電気泳動学会
  • Place of Presentation: 横浜
• [Presentation] プロテオーム解析に利用可能な病理解剖組織バンク(病理解剖バイオバンク)事業の開始 (2011)
  • Author(s): 沢辺元司
  • Organizer: 日本ヒトプロテオーム機構第9回大会(日本プロテオーム学会2011年会)
  • Place of Presentation: 新潟
• [Presentation] Neural stem sphere法によるサルの初期神経発生のプロテオーム解析 (2010)
  • Author(s): 赤間邦子、堀越朋恵、中山 孝、大津昌弘、今泉紀明、中村 愛、戸田年総、近藤 靖、鈴木 豊、井上順雄
  • Organizer: 第53回日本神経化学会大会
  • Place of Presentation: 神戸
• [Presentation] 酸化ストレスによるラミンのリン酸化と分子シャペロン複合体形成のプロテオーム解析 (2010)
  • Author(s): 戸田年総、中村 愛
  • Organizer: 日本電気泳動学会第62回総会
  • Place of Presentation: 札幌
• [Presentation] 脳脊髄液タンパク質の酸化還元状態の多角的プロテオーム解析 (2010)
  • Author(s): 戸田年総、岩本真知子、森澤 拓、吉澤利弘、芝崎 太
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会連合大会
  • Place of Presentation: 神戸
• [Presentation] ラット白色および褐色脂肪組織におけるカロリー制限に体する応答性の違い (2010)
  • Author(s): 林田雄介、福島麻由美、小島由美子、沖田直之、中村愛、戸田年総、樋上賀一
  • Organizer: 日本基礎老化学会第33回大会
  • Place of Presentation: 名古屋
• [Presentation] プロテオーム用研究情報管理システムの改良とGelML (2010)
  • Author(s): 森澤拓、廣田三佳子、戸田年総
  • Organizer: 日本ヒトプロテオーム機構第8回大会・第6回日本臨床プロテオーム研究会連合大会
  • Place of Presentation: 浦安
• [Presentation] ブタ精子成熟過程におけるユビキチン化タンパク質の効率的抽出濃縮法 (2010)
  • Author(s): 今泉紀明、井沼道子、平野久、中村愛、戸田年総、肥後大輔、赤間邦子
  • Organizer: 日本ヒトプロテオーム機構第8回大会・第6回日本臨床プロテオーム研究会連合大会
  • Place of Presentation: 浦安
• [Presentation] O-GlcNAc-Modification of the Proteins in Diabetic Cornea (2010)
  • Author(s): kimoto Y, Yuri Miura Y, Toda T, Yamamoto K, Munetomo E, Hart G W, Endo T, Hayato Kawakami H.
  • Organizer: Glyco T 2010 (7th International Symposium on Glyco syltransferase)
  • Place of Presentation: 東京
• [Presentation] 酸化ストレスによるラミンのリン酸化と分子シャペロン複合体形成のプロテオーム解析 (2010)
  • Author(s): 戸田年総、中村愛
  • Organizer: 第61回日本電気泳動学会総会
  • Place of Presentation: 札幌
• [Presentation] 新規アポトーシス誘導剤リダイフェン-Gの薬物標的分子の同定 (2010)
  • Author(s): 羽鳥麻奈美、森田明典、椎名勇、中田健也、戸崎雄太、王エンブン、戸田年総、大篭友博、矢守隆夫、池北雅彦
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会連合大会
  • Place of Presentation: 神戸
• [Presentation] Protein isoasparatyl methyltransferase gene knock down induced human alveolar epithelial to mesenchymal cell transition through endoplasmic reticulum stress (2010)
  • Author(s): 小笠原正人、戸田年総、韮澤悟、前山一隆、高橋砂織、山内広平
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会連合大会
  • Place of Presentation: 神戸
• [Presentation] 酸化ストレス負荷におけるSH-SY5Y細胞のブルーネイティブ二次元電気泳動によるプロテオーム解析(II) (2009)
  • Author(s): 中村愛, 森澤拓, 戸田年総
  • Organizer: 日本ヒトプロテオーム機構第7回大会
  • Place of Presentation: 東京
• [Presentation] 質量分析データビュワーの改良--プロテオーム研究情報管理のために-- (2009)
  • Author(s): 森澤拓, 中村愛, 廣田三佳子, 戸田年総
  • Organizer: 日本ヒトプロテオーム機構第7回会
  • Place of Presentation: 東京
• [Presentation] Proteome Analysis of Carbonyl Proteins in Cerebrospinal Fluid by Cy5-Hydrazide Labeling (2009)
  • Author(s): Tosifusa Toda, Megumi Nakamura, Futoshi shibasaki, Toshihiro Yoshizawa
  • Organizer: HUPO 8th Annual World Congress
  • Place of Presentation: Tronto、Canada
• [Presentation] Neural StemSphere法を用いたサルES細胞から神経幹細胞への分化過程における発現量変化解析 (2009)
  • Author(s): 今泉紀明, 堀越朋恵, 中山孝, 大津昌弘,赤間邦子, 中村愛, 戸田年総, 近藤靖, 鈴木豊, 井上順雄
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] 二次元目にTris-Tricine緩衝液系SDS-電気泳動を用いた場合のスタッキング効果に影響を及ぼす緩衝液条件の検討:ミニゲル二次元電気泳動のための泳動条件の最適化 (2009)
  • Author(s): 戸田年総, 中村愛, 廣田三佳子, 森澤拓
  • Organizer: 第60回日本電気泳動学会総会
  • Place of Presentation: 松本
• [Presentation] Tokyo Metropolitan Geriatric Hospital Autopsy Resource (TMGH-AR) (2009)
  • Author(s): Motoji Sawabe, Tomio Arai, Yuko Saito, Takahiko Shimizu, Tosifusa Toda, Shigeo Murayama, Masashi Tonaka, Kaiyo Takubo, Makiko Naka, Noriko Tanaka
  • Organizer: 19th IAGG World Congress of Gerontology and Geriatrics
  • Place of Presentation: Paris、France
• [Presentation] Release of New Web Site for Tokyo Metropolitan Geriatric Hospital Autopsy Resource(TMGH-AR) (2009)
  • Author(s): Sawabe M, Arai T, Saito Y, Shimizu T, Toda T, Murayama S, Tanaka M, Tokubo K, Naka M, Tanaka N
  • Organizer: ISBER 2009 Annual Meeting & Exhibits
  • Place of Presentation: Portland, Oregon, USA
• [Presentation] Tokyo Metropolitan Geriatric Hospital Autopsy Resource(TMGH-AR) (2009)
  • Author(s): Sawabe M, Arai T, Saito Y, Shimizu T, Toda T, Murayama S, Tanaka M, Tokubo K, Naka M, Tanaka N
  • Organizer: 19th IAGG World Congress of Gerontology and Geriatrics
  • Place of Presentation: Paris、France
• [Presentation] Neural Stem Sphere 法を用いたサルES細胞から神経への分化における発現比較解析 (2009)
  • Author(s): 今泉紀明, 堀越朋恵, 中山孝, 大津昌弘, 赤間邦子, 中村愛, 戸田年総, 近藤靖, 鈴木豊, 井上順雄
  • Organizer: 日本ヒトプロテオーム機構第7回大会
  • Place of Presentation: 東京
• [Presentation] 質量分析データビュワーの改良-プロテオーム研究情報管理のために- (2009)
  • Author(s): 森澤拓, 中村愛, 廣田三佳子, 戸田年総
  • Organizer: 日本ヒトプロテオーム機構第7回大会
  • Place of Presentation: 東京
• [Presentation] 二時限目に Tris-Tricine 緩衝液系SDS-電気泳動を用いた場合のスタッキング効果に影響を及ぼす緩衝液条件の検討 (2009)
  • Author(s): 戸田年総, 中村愛, 廣田三佳子, 森澤拓
  • Organizer: 第60回日本電気泳動学会総会
  • Place of Presentation: 松本
• [Presentation] Proteome Analysis of Carbonyl Proteins in Cerebrospinal Fluid by Cy5-Hyedrazide Labeling (2009)
  • Author(s): Toda T, Nakamura M, Shibasaki FT, Yoshizawa T.
  • Organizer: HUPO 8th Annual World Congress
  • Place of Presentation: Tronto、Canada
• [Presentation] ヒト唾液中に存在する2種類のエキソソームのプロテオーム解析 (2009)
  • Author(s): 小川裕子, 三浦ゆり, 原園景, 金井正美, 戸田年総, 遠藤玉夫, 山口照英, 秋元義弘, 川上速人, 津吹政可, 矢ノ下良平
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] Neural Stem Sphere 法を用いたサルES細胞から神経幹細胞への分化過程における発現量変化解析 (2009)
  • Author(s): 今泉紀明, 堀越朋恵, 中山孝, 大津昌弘, 赤間邦子, 中村愛, 戸田年総, 近藤靖, 鈴木豊, 井上順雄
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] シアニン系蛍光標識試薬を用いたタンパク質フリーチオールレベルの定量分析法に関する基礎的検討 (2008)
  • Author(s): 戸田年総, 中村愛, 森澤拓, 岩本真知子
  • Organizer: 第59回日本電気泳動学会総会
  • Place of Presentation: 東京
• [Presentation] An approach to the quantitative analysis of free thiol levels in human CSF proteins by the method of 2-D gel fluoroimaging with using cyanine dyes and fluorescent gel stain (2008)
  • Author(s): Tosifusa Toda, Megumi Nakamura, Hiraku Morisawa, Machiko Iwamoto, Junpei Ohkata, Hajime Sato, Hiroshi Tanaka, Kazutomi Kanemaru
  • Organizer: MPSA2008 17th Meeting of Methods in Protein Structure Analysis
  • Place of Presentation: 札幌
• [Presentation] Development of Autopsy Bioresource for Medical Research (2008)
  • Author(s): Sawabe M, Arai T, Kasahara I, Saito Y, Toda T, et al.
  • Organizer: ISBER 2008 Annual Meeting & Exhits
  • Place of Presentation: Bethesda, Maryland, USA
• [Remarks]
  • URL: http://proteome.tmig.or.jp/2D/
• [Remarks]
  • URL: http://proteome.tmig.or.jp/2D/