STR genotyping of the DNA fragments from fingerprint-lines captured by using a laser microdissection method

• Project/Area Number: 20590684
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Saitama Medical University
• Principal Investigator: HARA Masaaki Saitama Medical University, 医学部, 講師 (50129160)
• Co-Investigator(Kenkyū-buntansha): 高田 綾 埼玉医科大学, 医学部, 講師 (30245196) ; 斎藤 一之 (齋藤 一之) 埼玉医科大学, 医学部, 教授 (10215535)
• Co-Investigator(Renkei-kenkyūsha): SAITO Kazuyuki 埼玉医科大学, 医学部, 教授 (10215535) ; TAKADA Aya 埼玉医科大学, 医学部, 講師 (30245196)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: レーザーマイクロダイゼクション / 指紋 / STR / リアルタイムPCR / 指紋隆線 / DNA抽出 / レーザーマイクロダイゼクッション / 個人識別 / 物体検査

Research Abstract

The short tandem repeat (STR) genotyping of the extracted DNA from the single print of tape adhere the fingerprint (200mm^2) were performed, resulted in STR genotyping of the single print could be identified easily. Next study, to reveal the DNA extracted from refined single line of fingerprint on the microslide glass, STR genotyping of DNA sample from the single line was performed. A STR genotyping was identifiable 12～6 luci in 10x6mm, 7～2 loci in 7x4mm and 4～1 loci in 7x2mm.

Research Products

• [Journal Article] A Simple Identification Method of Saliva by Detecting Streptococcus salivarius Using Loop-mediated Isothermal Amplification. (2011)
  • Author(s): Hiroaki Nakanishi, Takeshi Ohmori, Masaaki Hara, Aya Takada, Hideki Shojo, Noboru Adachi, Kazuyuki Saito
  • Journal Title: JForencic Science 56 S1 Pages: 158-161
  • Peer Reviewed
• [Journal Article] クマネズミ胃内容物からのヒトSTR 型解析の検討 (2010)
  • Author(s): 原正昭, 高田綾, 斎藤一之, 木戸啓, 亀山浩, 山本靖久
  • Journal Title: DNA 多型 Vol.18 Pages: 144-147
  • NAID: 10029668204
  • Peer Reviewed
• [Journal Article] クマネズミ胃内容物からのヒトSTR型解析の検討 (2010)
  • Author(s): 原正昭, 他5名
  • Journal Title: DNA多型 Volume: 18 Pages: 144-147
  • NAID: 10029668204
  • Peer Reviewed
• [Journal Article] アジア人3集団におけるDXS6789座位の構造多型 (2010)
  • Author(s): 永井淳, 他3名
  • Journal Title: DNA多型 Volume: 18 Pages: 191-193
  • Peer Reviewed
• [Journal Article] A novel method for the identification of saliva by detecting oral streptococci using PCR. (2009)
  • Author(s): Hiroaki Nakanishi, Akira Kido, Takeshi Ohmori, Aya Takada, Masaaki Hara, Noboru Adachi, Kazuyuki Saito
  • Journal Title: Forensic Science International 183 Pages: 20-23
  • Peer Reviewed
• [Journal Article] STR genotyping of extracted from used Triage kits. (2009)
  • Author(s): Masaaki Hara, Akira Kido, Aya Takada, Takasi Miyazaki, Kazuyuki Saito
  • Journal Title: Forensic Science International : Genetics Supplement Series 2 Pages: 157-158
  • Peer Reviewed
• [Journal Article] Sequence polymorphisms at the DXS6789, DXS8377 and DXS101 loci in Asian populations. (2009)
  • Author(s): Atsushi Nagai, Masaaki Hara, Akira Kido, Aya Takada, Kazuyuki Saito, Yasuo Bunai
  • Journal Title: Forensic Science International : Genetics Supplement Series 2 Pages: 49-50
  • Peer Reviewed
• [Journal Article] STR and Y-STR genotyping assays of 25-year-old semen stains. (2008)
  • Author(s): M Hara, A Kido, H Kameyama, A Kido, T Miyazaki, K Saito
  • Journal Title: Forensic Science International, Genetics Supplement Series 1 Pages: 428-429
  • Peer Reviewed
• [Journal Article] A novel method for efficient analysis of STR loci from a single sperm captured by laser microdissection. (2008)
  • Author(s): T. Miyazaki, M.Hara, A. Ichiki, Y. Yamamoto, A.Takada, A.Kido, M. Nodera, H. Yanagisawa, H. Suzuki, K Saito
  • Journal Title: Forensic Science International, Genetics Supplement Series 1 Pages: 437-438
  • Peer Reviewed
• [Presentation] DXS10135 およびDXS10146 の構造多型 (2010)
  • Author(s): 永井淳
  • Organizer: 日本DNA 多型学会第19回学術集会
  • Place of Presentation: 静岡
  • Year and Date: 2010-11-19
• [Presentation] クマネズミの食壊により顔面部のみが白骨化した一剖検例 (2010)
  • Author(s): 原正昭
  • Organizer: 第94次日本法医学会学術全国集会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-25
• [Presentation] クマネズミの食壊により顔面部のみが白骨化した一剖検例 (2010)
  • Author(s): 原正昭, 他6名
  • Organizer: 日本法医学会学術全国集会
  • Place of Presentation: タワーホール船堀(東京)
  • Year and Date: 2010-06-25
• [Presentation] STR genotyping of extracted from used Triage kits. (2009)
  • Author(s): 原正昭
  • Organizer: 23^<rd> World Congress. International Society for Forensic Genetics
  • Place of Presentation: Buenos Aires, Argentina
  • Year and Date: 2009-09-16
• [Presentation] Sequence polymorphisms at the DXS6789, DXS8377 and DXS101 loci in Asian populations. (2009)
  • Author(s): 永井淳
  • Organizer: 23^<rd> World Congress. International Society for Forensic Genetics
  • Place of Presentation: Buenos Aires, Argentina
  • Year and Date: 2009-09-16
• [Presentation] Genetic studies of eight X-STRs in the Japanese population. (2008)
  • Author(s): Tamura A
  • Organizer: 7^<th> International Symposium Advances in Legal Medicine.
  • Place of Presentation: Osaka Japan
  • Year and Date: 2008-09-05
• [Presentation] Genetic data on Y-chromosome STR loci in Japanese, Bangladeshis, and Indonesians using an AmpFlSTR Yfiler kit. (2008)
  • Author(s): 原正昭
  • Organizer: DNA in Forensics 2008
  • Place of Presentation: Ancona Italy
  • Year and Date: 2008-05-30