| Project/Area Number |
20591315
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | University of Toyama |
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Principal Investigator |
ASANO Yukie (2009-2010) University of Toyama, 大学病院, 助教 (40420327)
渡辺 宏数 (2008) University of Toyama, 大学病院, 助教 (20399950)
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| Co-Investigator(Kenkyū-buntansha) |
NORISUGI Osamu 富山大学, 大学病院, 助教 (90419333)
浅野 幸恵 富山大学, 大学病院, 助教 (40420327)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | MIF / サイトカイン / ケモカイン / 表皮細胞 / 増殖 / 分化 / 炎症 / 紫外線 / マクロファージ / 細胞増殖 / 炎症性サイトカイン / フィラグリン |
|---|
| Research Abstract |
We examined the role of Macrophage migration inhibitory factor (MIF) in the proliferation and differentiation of theepidermis. The expression of cytokeratin 14 increased in normal human epidermal kerationocytes (NHEKs) or HaCaT cells after the addition of recombinant MIF. On the other hand, we observed decrease expression of of filaggrin and transglutaminase 1 in NHEKs and HaCaT cells after the addition of recombinant MIF. The cell growth increased by the addition of MIF and decreased after the transfection of MIF siRNA. Furthermore, it was suggested that MIF might promote the proliferation and suppress the differentiation of epidermis via Rho-GTPase, PI3K and ERK1/2.
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