| Project/Area Number |
20591687
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Ehime University |
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Principal Investigator |
KUMON Yoshiaki Ehime University, 大学院・医学系研究科, 准教授 (80127894)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Hideaki 愛媛大学, 大学院・医学系研究科, 講師 (30322275)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 脳梗塞 / 浸潤細胞 / 神経栄養因子 / 治療 / 脳虚血 / マクロファージ / 神経保護因子 |
|---|
| Research Abstract |
We found that macrophage-like cells accumulate in the ischemic core of a rat brain whose right middle cerebral artery was transiently occluded for 90 minutes. Many of these cells expressed Iba1, a marker of macrophages/microglias, as well as NG2 chondroitin sulfate proteoglycan (NG2), which has been recognized as a marker of oligodendrocyte progenitor cells. Such macrophage-like cells were termed BINCs (brain Iba1+/NG2+cells). BINCs were highly proliferative and their number peaked at 7 days post-reperfusion. Taken the various function of NG2, BINCs may be involved in not only phagocytosis of degenerated cells but also the healing and regeneration of lesion cores. 5-Fluorouracil (5FU) injection at 2 days post reperfusion markedly reduced the number of BINCs at 7 days post reperfusion, causing enlargement of necrotic volumes and frequent death of the rats. When isolated BINCs were transplanted into 5FU-aggravated ischemic lesion, the volume of the lesion was much reduced. Bone marrow-derived BINCs play a benefical role in ischemic brain lesions, at least in part, through secretion of neuroprotective factors.
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