Studies in targets of SYT-SSX fusion protein in synovial sarcoma.

• Project/Area Number: 20591756
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Okayama University
• Principal Investigator: OUCHIDA Mamuru Okayama University, 大学院・医歯薬学総合研究科, 准教授 (80213635)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 滑膜肉腫 / 染色体転座 / 融合遺伝子 / SYT / SS18 / SSX / 転写制御

Research Abstract

The aim of this study was identification of the target genes transcriptionally regulated by SYT-SSX fusion protein in synovial sarcomas. We found about 300 candidate targets by chromatin immunoprecipitation assay, DNA cloning and sequencing. We have screened the reagents which can block and inhibit the localization of SYT-SSX fusion protein in synovial sarcoma cells, to find new targeted therapy agents for synovial sarcoma.

Research Products

• [Journal Article] The Effect of SYT-SSX and Extracellular Signal-Regulated Kinase (ERK) on Cell Proliferation in Synovial Sarcoma. (2011)
  • Author(s): Cai W, Sun Y, Wang W, Han C, Ouchida M, Xia W, Zhao X, Sun B.
  • Journal Title: Pathol Oncol Res. (in press)[Epub ahead of print]
  • Peer Reviewed
• [Journal Article] Frequent methylation of RASSF1A in synovial sarcoma and the anti-tumor effects of 5-aza-2'-deoxycytidine against synovial sarcoma cell lines. (2010)
  • Author(s): Numoto K, Yoshida A, Sugihara S, Kunisada T, Morimoto Y, Yoneda Y, Fujita Y, Nishida K, Ouchida M, Ozaki T.
  • Journal Title: J Cancer Res Clin Oncol. 136 Pages: 17-25
  • Peer Reviewed
• [Journal Article] Frequent methylation of RASSF1A in synovial sarcoma and the anti-tumor effects of 5-aza-2'-deoxycytidine against synovial sarcoma cell lines. (2010)
  • Author(s): Kunihiko Numoto
  • Journal Title: J Cancer Res Clin Oncol. 136 Pages: 17-25
  • Peer Reviewed
• [Presentation] Identification of target genes of miR-17-92 cluster by proteomic analysis. (2010)
  • Author(s): 大内田守
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] Proteomic analysis of target proteins of miR-17-92 cluster in cancer cells (2010)
  • Author(s): 大内田守
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-22
• [Presentation] 進む癌遺伝子検査について (2010)
  • Author(s): 大内田守
  • Organizer: 福山市民公開講座
  • Place of Presentation: 福山
  • Year and Date: 2010-06-06
• [Presentation] Identification of target genes of miR-17-92 cluster by proteomic analysis. (2010)
  • Author(s): Ouchida M, Kanzaki H, Ito S、Yamamoto K, Tamaru S, Shimizu K
  • Organizer: 第83回日本分子生物学会総会
  • Place of Presentation: 神戸
• [Presentation] Proteomic analysis of target proteins of miR-17-92 cluster in cancer cells. (2010)
  • Author(s): Ouchida M, Kanzaki H, Ito S, Shimizu K
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
• [Presentation] 進む癌遺伝子検査について (2009)
  • Author(s): 大内田守
  • Organizer: 財団法人福山通運渋谷長寿健康財団・脳神経センター大田記念病院主催福山市民公開講座 招待講演
  • Place of Presentation: 福山
  • Year and Date: 2009-06-06
• [Presentation] 滑膜肉腫関連蛋白の細胞内局在に影響を与える薬剤、及び蛋白に関する検討 (2008)
  • Author(s): 米田 泰史
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-10-23
• [Presentation] ヒストン脱アセチル化酵素阻害剤valproic acidと脱メチル化剤zebularineの併用による滑膜肉腫細胞株に対する抗腫瘍効果の検討 (2008)
  • Author(s): 佐藤 千明
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-10-23
• [Presentation] 骨肉腫患者における葉酸代謝関連遺伝子の一塩基多型の検討 (2008)
  • Author(s): 森本裕樹、吉田晶、大内田守、国定俊之、清水憲二、尾崎敏文
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] The influence of various drugs and proteins on the localization of synovial sarcoma-related proteins (2008)
  • Author(s): 米田泰史、国定俊之、吉田晶、佐々木剛、森本裕樹、尾崎敏文、大内田守、神崎浩孝、清水憲二
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
• [Presentation] Antitumorgenic effect of the combination therapy with the histone deacetylase inhibitor, valproic acid and the methylation inhibitor, zebularine in synovial sarcoma cell (2008)
  • Author(s): 佐藤千明、吉田晶、森本裕樹、米田泰史、佐々木剛、国定俊之、大内田守、尾崎敏文
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
• [Remarks] ホームページ
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