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Elucidation of the intracellular mechanisms concerning monocyte tissue factor production and release, and their applications of the gene therapy

Research Project

Project/Area Number 20591842
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

NAKAJIMA Yasufumi  Kyoto Prefectural University of Medicine, 医学研究科, 助教 (70326239)

Co-Investigator(Kenkyū-buntansha) UENO Hiroshi  京都府立医科大学, 医学研究科, 助教 (20381965)
MIZOBE Toshiki  京都府立医科大学, 医学研究科, 講師 (50239266)
HASHIMOTO Satoru  京都府立医科大学, 医学研究科, 准教授 (90167578)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsRNA干渉法 / 遺伝子治療 / 静脈性血栓塞栓症 / 周術期
Research Abstract

We have demonstrated the modification of tissue factor expression on THP-1 cell and mouse monocyte-macrophage by knocking down ERK1, ERk-1 and Egr-1. With injection of these gene knocked down monocytes into mouse, survival rate and severity of pulmonary embolism have improved in mouse experimental model. Further studies are necessary to undergo several analysis, and their application of the gene therapy.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (25 results)

All 2010 2009 2008

All Journal Article (11 results) (of which Peer Reviewed: 10 results) Presentation (12 results) Book (2 results)

  • [Journal Article] How much dosage of heparin do you administer? The relationship between heparin dosage and activated coagulation time (ACT) value during cardiovascular surgery: a multi center investigations.2010

    • Author(s)
      Shibasaki M, Nakajima Y, Kojima A, Kitagawa H, Tanimoto K, Mizobe T
    • Journal Title

      Masui 59

      Pages: 535-539

    • NAID

      40017090699

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prediction of pediatric endotracheal tube size by ultrasonography.2010

    • Author(s)
      Shibasaki M, Nakajima Y, Ishii S, Shimizu F, Shime N, Sessler DI.
    • Journal Title

      Anesthesiology 113

      Pages: 819-824

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 特集「ペインクリニックと抗血栓療法の基礎と臨床~抗血栓療法を受けている患者に安全に神経ブロックを行うために~」によせて2010

    • Author(s)
      高橋正裕, 古家仁, 中嶋康文, 細川豊史
    • Journal Title

      ペインクリニック 31(10)

      Pages: 1271-1272

    • Related Report
      2010 Final Research Report
  • [Journal Article] Prediction of pediatric endotracheal tube size by ultrasonography.2010

    • Author(s)
      Shibasaki M, Nakajima Y, Ishii S, Shimizu F, Shime N, Sessler DI.
    • Journal Title

      Anesthesiology.

      Volume: 113 Pages: 819-24

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anaphylaxis during anesthesia-especially from neuromuscular blocking agents.2010

    • Author(s)
      Kageyama K, Hayakawa Y, Nakajima Y, Hashimoto S.
    • Journal Title

      Masui

      Volume: 60 Pages: 55-66

    • NAID

      40017424754

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] How much dosage of heparin do you administer? The relationship between heparin dosage and activated coagulation time (ACT) value during cardiov ascular surgery : a multi center investigations.2010

    • Author(s)
      Shibasaki M, Nakajima Y, Kojima A, Kitagawa H, Tanimoto K, Mizobe T.
    • Journal Title

      Masui

      Volume: 59 Pages: 535-9

    • NAID

      40017090699

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphodiesterase 3 inhibition reduces platelet activation and monocyte tissue factor expression in knee arthroplasty patients.2009

    • Author(s)
      Beppu, S., Nakajima, Y, Shibasaki, M., Kageyama, K., Mizobe, T., Shime, N., Matsuda, N.
    • Journal Title

      Anesthesiology 111

      Pages: 1227-1237

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphodiesterase 3 inhibition reduces platelet activation and monocyte tissue factor expression in knee arthroplasty patients.2009

    • Author(s)
      Beppu S, Nakajima Y, et al.
    • Journal Title

      Anesthesiology 111

      Pages: 1227-1237

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fas ligand released by activated monocytes causes apoptosis of lung epithelial cells in human acute lung injury model in vitro.2008

    • Author(s)
      Mizuta M, et al.
    • Journal Title

      Biol Pharm Bull. 31

      Pages: 386-90

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intraoperative monitoring of movement of an entrapped coronary guidewire by transesophageal echocardiography.2008

    • Author(s)
      Hosokawa K, et al.
    • Journal Title

      Anesth Analg. 107

      Pages: 1158-60

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An evaluation of acute cardiac tamponade by transesophageal echooardiography.2008

    • Author(s)
      Hosokawa K, et. al.
    • Journal Title

      Anesth Analg. 106

      Pages: 61-2

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 周術期の血小板変化とその対策2010

    • Author(s)
      中嶋康文
    • Organizer
      平和物産社内勉強会
    • Place of Presentation
      東京 教育講演
    • Year and Date
      2010-10-09
    • Related Report
      2010 Final Research Report
  • [Presentation] 周術期における血小板変化と血栓-止血の制御2010

    • Author(s)
      中嶋康文
    • Organizer
      日本麻酔科学会関西地方会
    • Place of Presentation
      大阪 教育講演
    • Year and Date
      2010-09-04
    • Related Report
      2010 Final Research Report
  • [Presentation] Time-dependent changes in intraplatelet signaling pathways relevant to platelet function defect with cardiopulmonary bypass surgery.2010

    • Author(s)
      Murase M, Nakajima Y, Sessler DI, Shibasaki M, Mizobe.T.
    • Organizer
      Euroanesthesia 2010.
    • Place of Presentation
      Helsinki, Finland.
    • Year and Date
      2010-06-12
    • Related Report
      2010 Final Research Report
  • [Presentation] Time-dependent changes in intraplatelet signaling pathways relevant to platelet function defect with cardiopulmonary bypass surgery2010

    • Author(s)
      Murase M, Nakajima Y, et al.
    • Organizer
      Euroanaesthesia 2010
    • Place of Presentation
      Helsinki
    • Year and Date
      2010-06-12
    • Related Report
      2010 Annual Research Report
  • [Presentation] 周術期血小板研究における最新の話題(シンポジウム血液凝固・炎症反応の関連と臓器障害)2010

    • Author(s)
      中嶋康文
    • Organizer
      第57回日本麻酔科学会
    • Place of Presentation
      福岡
    • Year and Date
      2010-06-04
    • Related Report
      2010 Final Research Report
  • [Presentation] Time-dependent changes in intraplatelet signaling pathways relevant to platelet function defect with cardiopulmonary bypass surgery.2010

    • Author(s)
      Nakajima Y, Murase M, Shibasaki M, Kageyama K, Matsuda N
    • Organizer
      13th Asian Australasian Congress of Anesthesiologists.
    • Place of Presentation
      Fukuoka, Japan.
    • Year and Date
      2010-06-04
    • Related Report
      2010 Final Research Report
  • [Presentation] Making a transgenic mouse with alpha2A adrenergic receptor fused with green fluorescent protein.2010

    • Author(s)
      Mizobe T, Nakajima Y, Nishi M
    • Organizer
      13th Asian Australasian Congress of Anesthesiologists
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2010-06-04
    • Related Report
      2010 Final Research Report
  • [Presentation] 開心術患者の血小板機能に関する細胞内情報伝達系の時系列解析2010

    • Author(s)
      村瀬百子、中嶋康文、柴崎雅志、廣瀬宗孝、影山京子、松田直之
    • Organizer
      日本麻酔科学会第57回学術集会
    • Place of Presentation
      福岡(優秀演題)
    • Year and Date
      2010-06-04
    • Related Report
      2010 Final Research Report
  • [Presentation] 人工膝関節形成術周術期におけるミルリノンの血小板活性正常化作用-全血を用いた血小板機能測定-2009

    • Author(s)
      別府賢, 中嶋康文, 村瀬百子, 柴崎雅志,志馬伸朗, 松田直之
    • Organizer
      第56回日本麻酔科学会学術集会
    • Place of Presentation
      神戸
    • Year and Date
      2009-08-17
    • Related Report
      2010 Final Research Report
  • [Presentation] 血小板細胞内情報伝達に対するヒドロキシエチルスターチの影響2009

    • Author(s)
      稲見直子, 中嶋康文, 別府賢, 柴崎雅志, 溝部俊樹, 松田直之
    • Organizer
      第56回日本麻酔科学会学術集会
    • Place of Presentation
      神戸(優秀演題)
    • Year and Date
      2009-08-16
    • Related Report
      2010 Final Research Report
  • [Presentation] 血小板細胞内情報伝達に対するヒドロキシエチルスターチの影響2009

    • Author(s)
      稲見直子、中嶋康文、影山京子
    • Organizer
      日本麻酔科学会学術集会
    • Place of Presentation
      神戸
    • Year and Date
      2009-08-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒドロキシエチルスターチ(HES)輸液製剤が血液細胞に及ぼす炎症惹起性の解明2008

    • Author(s)
      稲見直子
    • Organizer
      日本麻酔科学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2008-06-12
    • Related Report
      2008 Annual Research Report
  • [Book] 徹底ガイド心臓麻酔Q&A(大動脈弁手術の麻酔管理)(澄川耕二編(分担執筆) 東京2009

    • Author(s)
      柴崎雅志, 中嶋康文, 溝部俊樹
    • Publisher
      総合医学社
    • Related Report
      2010 Final Research Report
  • [Book] 周術期麻酔管理ハンドブック2008

    • Author(s)
      小栗顕二, 横野諭編分担執筆
    • Total Pages
      649
    • Publisher
      金芳堂
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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