| Project/Area Number |
20592158
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
KURABUCHI Shingo The Nippon Dental University, 生命歯学部, 准教授 (90170076)
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| Co-Investigator(Kenkyū-buntansha) |
HOSOI Kazuo 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (10049413)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 唾液腺 / 顆粒性導管 / アンドロゲン / 甲状腺ホルモン / ホルモン受容体 / 免疫組織化学 / 表現型 / マウス / Androgen / 電子顕微鏡 |
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| Research Abstract |
Granular convoluted tubule (GCT) -like cells in the submandibular gland (SMG), were induced in the striated ducts (SDs) of the parotid gland (PAG) of mouse after the repeatedly injections of androgen and thyroid hormone. This finding and our serial studies suggest that the hormone-dependent GCT is a common characterization in murine three major salivary glands. However, the process of cytodifferentiation from immature granular cells to GCT cells will be varying in each gland. This process is completed in a large population of SD cells in the SMG, with a small population restricted to the sublingual gland (SLG), but all of the SD cells maintain their original form in the PAG. The gland-specific GCT-phenotype might be caused by contents of hormone receptors. However, we have tried unsuccessfully to detect immunolocalization and western blotting of androgen receptor (AR) in the mouse salivary glands, using several commercially available antibodies for ARs. It is conceivable that molecular structures of ARs may be different between the salivary glands and the reproductive organs.
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