Imaging analysis of the electrolyte transport and protein secretions in salivary ducts to reveal their regulatory mechanisms
| Project/Area Number |
20592180
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MORITA Takao 北海道医療大学, 歯学部, 講師 (20326549)
NEZU Akihiro 北海道医療大学, 歯学部, 講師 (00305913)
TOJYO Yosuke 北海道医療大学, 歯学部, 教授 (90111731)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 歯科薬理学 / 唾液腺 / 導管 / カルシウム / 分泌 / イメージング / 蛍光タンパク質 / プリン受容体 / イノシトール三リン酸 / 蛍光蛋白質 |
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| Research Abstract |
Using multiphoton microscopy, the present study established that rat salivary ductal cells exhibit spontaneous oscillations in intracellular Ca^<2+> concentration([Ca^<2+>] i). Pharmacological examinations indicate that purinergic receptors play major roles in these spontaneous Ca2+oscillations. It was also found that spontaneous elevations of[Ca^<2+>] i were well correlated with spontaneous cell swelling of ductal cells. These results indicate the possibility that the Ca^<2+> oscillations are induced by the spontaneous ATP release in salivary ductal cells in the resting state and regulate electrolyte reabsorption.
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Report
(4 results)
Research Products
(33 results)
