The analysis of pathological condition in masticatory muscle tendon-aponeurosis hyperplasia using HUMARA assay and immunostaining methods.

• Project/Area Number: 20592344
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Saitama Medical University
• Principal Investigator: YODA Tetsuya 埼玉医科大学, 医学部, 教授 (60242210)
• Co-Investigator(Kenkyū-buntansha): SATO Tsuyoshi 埼玉医科大学, 医学部, 講師 (60406494) ; SAKATA Yasuaki 埼玉医科大学, 医学部, 講師 (50322425) ; 安部 貴大 埼玉医科大学, 医学部, 助教 (20383250)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 咀嚼筋腱腱膜過形成症 / クローナリティー解析 / 免疫染色 / 咀嚼筋腱・腱膜過形成症 / Tenomodulin / Smad8 / HUMARA assay / GDF-8 / HUMRA assay / 咀嚼筋腱・腱膜過形成症Tenomodulin

Research Abstract

We calculated seven samples in masticatory muscle tendon-aponeurosis hyperplasia(MATAPHY). We found that both Tenomodulin and Smad8 proteins expressed in MATAPHY's tendon and the proliferation of tendon was a polyclonal fashion. We also demonstrated that collagen 6A1 was upregulated in MATAPHY's tendon by using two dimensional electrophoresis system.

Research Products

• [Journal Article] Proteomic analysis of a masticatory muscle tendon-aponeurosis hyperplasia : a preliminary study using 2D-DIGE system (2012)
  • Author(s): Sato T, Nakamoto A, Hori N, Enoki Y, Fukushima Y, Nakamoto N, Sakata Y, Yamanaka H, Chida D, Abe T, Yoda T
  • Journal Title: J Oral Maxillofac Surg Med Pathol Volume: (in press)
  • Peer Reviewed
• [Presentation] 咀嚼筋腱腱膜過形成症におけるタンパク質プロファイリング (2010)
  • Author(s): 佐藤毅, 中本文, 安部貴, 大堀直子, 中本紀道, 福島洋介, 坂田康彰, 依田哲也
  • Organizer: 第23回日本顎関節学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-24
• [Presentation] 咀嚼筋腱腱膜過形成症におけるタンパク質プロファイリング (2010)
  • Author(s): 佐藤毅, 中本文, 安部貴大, 堀直子, 中本紀道, 福島洋介, 坂田康彰, 依田哲也
  • Organizer: 第23回日本顎関節学会学術大会
  • Place of Presentation: 東京, 船堀
  • Year and Date: 2010-07-24
• [Presentation] 咀嚼筋腱腱膜過形成症におけるタンパク質プロファイリング (2010)
  • Author(s): 佐藤毅
  • Organizer: 第23回日本顎関節学会
  • Place of Presentation: 船堀
  • Year and Date: 2010-07-24