Biological responses that eliminate invasive bacteria in the periodontal cells

• Project/Area Number: 20592432
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Nagasaki University
• Principal Investigator: KANEKO Takashi Nagasaki University, 医歯薬学総合研究科, 助教 (10284697)
• Co-Investigator(Kenkyū-buntansha): HARA Yoshitaka 長崎大学, 大学院・医歯薬学総合研究科, 教授 (60159100) ; YOSHIMURA Atsutoshi 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (70253680)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯周病原細菌 / ペプチドグリカン / NOD1 / NOD2 / サイトカイン / Nucleotide-binding oligomerization / Porphyromonas gingivalis / Aggregatibacter actinomycetemcomitans / Fusobacterium nucleatum / 自然免疫 / Porphylomonas gingivalis

Research Abstract

Some of periodontal pathogens have an ability to invade gingival epithelial cells. This ability is thought as one of the mechanism to induce periodontal inflammation. In the present study, we focus on NOD proteins that have been shown to play an important role on detection and elimination of invasive bacteria, and investigate whether NODs proteins can recognize periodontal pathogens or not. As results, we find both NOD1- and NOD2-stimulatory activities are different by the species of periodontal pathogens.

Research Products

• [Journal Article] 歯周組織破壊に導く歯周病原細菌-宿主問相互作用の解明 (2011)
  • Author(s): 金子高士
  • Journal Title: 日歯周誌 53(1) Pages: 3-12
• [Journal Article] 歯周組織破壊に導く歯周病原細菌-宿主間相互作用の解明 (2011)
  • Author(s): 金子高士
  • Journal Title: 日本歯周病学会会誌 Volume: (印刷中)
  • NAID: 10028051226
  • Peer Reviewed
• [Journal Article] NOD1 and NOD2 Mediate Sensing of Periodontal Pathogens. (2010)
  • Author(s): Okugawa T, Kaneko T, Yoshimura A, Silverman N, Hara Y.
  • Journal Title: J Dent Res 89(2) Pages: 186-191
• [Journal Article] Transcriptional regulation of bone sialoprotein gene by Porphyromonas gingivalis lipopolysaccharide. (2010)
  • Author(s): Li X, Kato N, Mezawa M, Li Z, Wang Z, Yang L, Sasaki Y, Kaneko T, Takai H, Yoshimura A, Ogata Y.
  • Journal Title: J Cell Biochem 110(4) Pages: 823-833
• [Journal Article] NOD1 and NOD2 mediate sensing of periodontal pathogens. (2010)
  • Author(s): Okugawa, et.al.
  • Journal Title: Journal of Dental Research 89 Pages: 186-191
  • Peer Reviewed
• [Journal Article] Activity of supragingival plaque to induce Toll-like receptor 4-mediated stimulation is associated with cytokine production by peripheral blood mononuclear cells. (2009)
  • Author(s): Yamaguchi R, Yoshimura A, Yoshioka H, Kaneko T, Hara Y.
  • Journal Title: J Periodontol 80(3) Pages: 512-520
  • NAID: 130004571075
• [Journal Article] Locally administered T cells from mice immunized with lipopolysaccharide (LPS) accelerate LPS-induced bone resorption. (2009)
  • Author(s): Ozaki Y, Ukai T, Yamaguchi M, Yokoyama M, Haro ER, Yoshimoto M, Kaneko T, Yoshinaga M, Nakamura H, Shiraishi C, Hara Y.
  • Journal Title: Bone 44(6) Pages: 1169-1176
• [Journal Article] Cyclosporin A and phenytoin modulate inflammatory responses. (2009)
  • Author(s): Suzuki AMM, Yoshimura A, Ozaki Y, Kaneko T, Hara Y.
  • Journal Title: J Dent Res 88(12) Pages: 1131-1136
• [Journal Article] Lack of Toll-like receptor 4 decreases lipopolysaccharide-induced bone resorption in C3H/HeJ mice in vivo. (2008)
  • Author(s): Nakamura H, Fukusaki Y, Yoshimura A, Shiraishi C, Kishimoto M, Kaneko T, Hara Y.
  • Journal Title: Oral Microbiol Immunol 23(3) Pages: 190-195
• [Journal Article] Analysis of the activity to induce TLR2- and TLR4-mediaed stimulation of supragingival plaque (2008)
  • Author(s): Yoshioka H, Yoshimura A, Kaneko T, Golenbock DT, Hara Y.
  • Journal Title: J Periodontol 79(5) Pages: 920-928
• [Journal Article] T cells are able to promote lipopolysaccharide-induced bone resorption in mice in the absence of B cells. (2008)
  • Author(s): Yamaguchi M, Ukai T, Kaneko T, Yoshinaga M, Yokoyama M, Ozaki Y, Hara Y.
  • Journal Title: J Periodontal Res 43(5) Pages: 549-555
  • NAID: 120006982397
• [Journal Article] グリチルレチン酸はLPS誘導性骨吸収を抑制する (2008)
  • Author(s): 中村弘隆、鵜飼孝、吉永泰周、尾崎幸生、金子高士、白石千秋、岸本真実、小野山美穂、阿部嘉裕、安部達也、吉村篤利、原宜興
  • Journal Title: 日本歯科保存学雑誌 51(3) Pages: 323-330
  • NAID: 110007151238
• [Presentation] NOD1 and NOD2 Mediate Cytopasmic Sensing of Periodontal Pathogens (2010)
  • Author(s): Takashi Kaneko, et al.
  • Organizer: 88^<th> General Session & Exhibition of the IADR
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-16
• [Presentation] ラット歯肉溝へのLPSおよび抗LPS IgGの交互滴下によりアタッチメントロスが誘導される (2010)
  • Author(s): 藏本明子, 金子高士, 吉永泰周, 鵜飼孝, 白石千秋, 岸本真実, 横山美穂, 岸本隆明, 市村育久, 押野一志, 原宜興
  • Organizer: 第53回春季日本歯周病学会学術大会、盛岡市民文化ホールおよびいわて県民情報交流センター(アイーナ)
• [Presentation] NODによる歯周病原細菌の認識 (2009)
  • Author(s): 金子高士, ら
  • Organizer: 第131回日本歯科保存学会 秋季学術大会
  • Place of Presentation: 仙台
  • Year and Date: 2009-10-30
• [Presentation] P.gingivalis lipopolysaccharide regulates bone sialoproteins gene transcription (2009)
  • Author(s): Li X, Kato N, Kaneko T, Yoshimura A, Ogata Y
  • Organizer: Europerio 6
  • Place of Presentation: Stockholm, Sweden
• [Presentation] 歯肉炎上プラークのTLR4刺激作用と末梢血単核球からのサイトカイン産生誘導能との関連 (2009)
  • Author(s): 吉村篤利、山口竜亮、吉岡英将、佐藤佳昌、金子高士、原宜興
  • Organizer: 第52回春季日本歯周病学会学術大会
  • Place of Presentation: 岡山市岡山コンベンションセンター
• [Presentation] Cyclosporin A and phenytoin differently modulate Toll-Like receptor-mediated responses in human gingival fibroblasts. (2009)
  • Author(s): Suzuki AMM, Yoshimura A, Kaneko T, Hara Y
  • Organizer: The 9^<th> World Congress on Inflammation.
  • Place of Presentation: Keio plaza hotel, Tokyo.
• [Presentation] NODによる歯周病原細菌の認識 (2009)
  • Author(s): 金子高士、吉村篤利、原宜興
  • Organizer: 第131回日本歯科保存学会2009年度秋季学術大会
  • Place of Presentation: 仙台市仙台国際センター
• [Presentation] NOD mediates cytoplasmic sensing of periodontal pathogens. (2008)
  • Author(s): Takashi Kaneko, et al.
  • Organizer: 10^<th> Biannuel IEIIS Meeting
  • Place of Presentation: Edinburgh, UK
  • Year and Date: 2008-07-30
• [Presentation] 歯周病原性細菌のNOD活性化能に関する研究 (2008)
  • Author(s): 奥川剛志、金子高士、吉村篤利、原宜興
  • Organizer: 第51回春季日本歯周病学会春季学術大会
  • Place of Presentation: 大宮
• [Presentation] Effects of Cyclosporin A on TLR-Mediated Responses of Gingival Fibroblasts. (2008)
  • Author(s): Suzuki AMM, Yoshimura A, Kaneko T, Hara Y.
  • Organizer: 第51回春季日本歯周病学会総会
  • Place of Presentation: 大宮
• [Presentation] P.gingivalis lipopolysaccharide regulates bone sialoprotein gene transcription. (2008)
  • Author(s): Li X, Kato N, Maezawa M, Araki S, Kaneko T, Yoshimura A, Ogata Y.
  • Organizer: 日本歯科保存学会第128回春季学術大会
  • Place of Presentation: 新潟
• [Presentation] NOD mediates cytoplasmic sensing of periodontal pathogens. (2008)
  • Author(s): Kaneko T, Okugawa T, Yoshinaga M, Kishimoto M, Yoshimura A, Hara Y.
  • Organizer: 10^<th> Biannual IEIIS Meeting, International Endotoxin and Innate Immunity Society Edinburgh
  • Place of Presentation: UK
• [Presentation] Identification of a cell surface component of Porphyromonas gingivalis that induces TLR2- and TLR4-independent activation of NF-kB in CHO cells. (2008)
  • Author(s): Kishimoto M, Yoshimura A, Naito M, Haruyama K, Kaneko T, Nakayama K, Hara Y.
  • Organizer: 10^<th> Biannual IEIIS Meeting, International Endotoxin and Innate Immunity Society Edinburgh
  • Place of Presentation: UK
• [Presentation] Analysis of the activity to induce TLR2- and TLR4-mediated stimulation of supragingival and subgingival plaque. (2008)
  • Author(s): Yoshimura A, Yoshioka H, Suzuki AMM, Kishimoto M, Kaneko T, Golenbock DT, Hara Y.
  • Organizer: 10^<th> Biannual IEIIS Meeting, International Endotoxin and Innate Immunity Society Edinburgh
  • Place of Presentation: UK
• [Presentation] Cyclosporin A modulates TLR-mediated responses in human gingival fibroblasts. (2008)
  • Author(s): Suzuki AMM, Yoshimura A, Kaneko T, Kishimoto M, Hara Y.
  • Organizer: 10^<th> Biannual IEIIS Meeting, International Endotoxin and Innate Immunity Society Edinburgh
  • Place of Presentation: UK
• [Presentation] 歯肉縁下プラークのToll-1ike receptor (TLR) 2およびTLR4刺激作用と歯周病臨床パラメーターの関連性について (2008)
  • Author(s): 吉村篤利, 吉岡英将, 山口竜亮, 金子高士, 原宜興
  • Organizer: 第129回日本歯科保存学会2008年度秋季学術大会
  • Place of Presentation: 富山市富山国際会議場
• [Presentation] NOD1 and NOD2 mediate cytoplasmic sensing of periodontal pathogens.
  • Author(s): Kaneko T, Okugawa T, Yoshimura A, Sato K, Hara Y.
• [Presentation] Immune complex formed in rat gingival sulcus induces attachment loss.
  • Author(s): Kuramot A, Yoshinaga Y, Ukai T, Kaneko T, Shiraishi C, Yokoyama M, Sato T, Nagano F, Takamori Y, Hara Y.
• [Presentation] An experimental model of periodontitis in LPS-Immunized rat.
  • Author(s): Yoshinaga Y, Kuramoto A, Kaneko T, Ukai T, Shiraishi C, Yoshinaga M, Sato T, Nagano F, Hara Y.
• [Presentation] Cyclosporin A and phenytoin modulate inflammatory responses in gingival fibroblasts.
  • Author(s): Yoshimura A, Suzuki AMM, Y. Ozaki Y, Sato K, Kaneko T, Hara Y.
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