Structure and Dynamics of Single Polymer Chain Studied by Three-dimensional Super-resolution Imaging Technique

• Project/Area Number: 20685016
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Polymer/Textile materials
• Research Institution: Kyoto University
• Principal Investigator: AOKI Hiroyuki Kyoto University, 先端医工学研究ユニット, 准教授 (90343235)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000); Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
• Keywords: 単一高分子鎖 / 表面・界面 / 蛍光顕微鏡 / 超解像 / 分子運動 / 高分子ダイナミクス

Research Abstract

A super-resolution microscopy technique was developed to enable the structural analysis of polymers at a nanometric scale. A single poly(butyl methacrylate) (PBMA) chain labeled by photochromic fluorescent dye molecules was observed by the photo-activated localization microscopy (PALM), which has the spatial resolution of 15×15×80nm^3 in three dimensions. The PALM allowed us to directly examine the conformation of the individual PBMA chains.

Research Products

• [Journal Article] Segmental Orientation of Single Polymer Chain in Langmuir-Blodgett Film Studied by Defocus Fluorescence Imaging (2011)
  • Author(s): Hiroyuki Aoki, Toshiaki Takahashi, Shinzaburo Ito
  • Journal Title: Polym.J. 43 Pages: 218-221
  • NAID: 10027789250
• [Journal Article] Segmental Orientation of Single Polymer Chain in Langmuir-Blodgett Film Studied by Defocus Fluorescence Imaging (2011)
  • Author(s): Hiroyuki Aoki, Toshiaki Takahashi, Shinzaburo Ito
  • Journal Title: Polym.J. Volume: 43 Pages: 218-221
  • NAID: 10027789250
  • Peer Reviewed
• [Journal Article] 単一分子計測による高分子鎖の構造評価 (2011)
  • Author(s): 青木裕之
  • Journal Title: 高分子 Volume: 60 Pages: 66-69
  • NAID: 10027789827
• [Journal Article] Conformational Relaxation of Single Polymer Chains Confined in Two-Dimensional Plane Studied by Scanning Near-Field Optical Microscopy (2010)
  • Author(s): Hiroyuki Aoki, Ryojun Sekine, Tatsuya Iwamoto, Shinzaburo Ito
  • Journal Title: Polym.J. 42 Pages: 124-130
  • NAID: 10026363961
• [Journal Article] Conformational Relaxation of Single PolymerChains Confined in Two-Dimensional Plane Studied by Scanning Near-Field Optical Microscopy (2010)
  • Author(s): H.Aoki, R.Sekine, T.Iwamoto, S.Ito
  • Journal Title: Polym.J. 42 Pages: 124-130
  • Peer Reviewed
• [Journal Article] Poly (methacrylate)s Labeled by Perylene Diimide: Synthesis and Applications in Single Chain Detection Studies (2009)
  • Author(s): Hiroyuki Aoki, Toshiaki Takahashi, Ryojun Sekine, Yasunari Tamai, Shinji Aoki, Keita Tani, Shinzaburo Ito
  • Journal Title: Polym.J. 41 Pages: 778-783
  • NAID: 10025525523
• [Journal Article] Conformation of Single Block Copolymer Chain in Two-Dimensional Microphase-Separated Structure Studied by Scanning Near-Field Optical Microscopy (2009)
  • Author(s): Ryojun Sekine, Hiroyuki Aoki, Shinzaburo Ito
  • Journal Title: J.Phys.Chem.B 113 Pages: 7095-7100
• [Journal Article] Affine Deformation of Single Polymer Chain in Poly (methyl methacrylate) Films under Uniaxial Extension Observed by Scanning Near-Field Optical Microscopy (2009)
  • Author(s): Toru Ube, Hiroyuki Aoki, Shinzaburo Ito, Jun-ichi Horinaka, Toshikazu Takigawa, Toshiro Masuda
  • Journal Title: Polymer 50 Pages: 3016-3021
• [Journal Article] Conformation of Single Homopolymer Chain in Micro-Phase Separated Block Copolymer Monolayer Studied by Scanning Near-Field Optical Microscopy (2009)
  • Author(s): Yasunari Tamai, Ryojun Sekine, Hiroyuki Aoki, Shinzaburo Ito
  • Journal Title: Macromolecules 42 Pages: 4224-4229
• [Journal Article] Chain End Distribution of Block Copolymer in Two-dimensional Microphase-Separated Structure Studied by Scanning Near-Field Optical Microscopy (2009)
  • Author(s): Ryojun Sekine, Hiroyuki Aoki, Shinzaburo Ito
  • Journal Title: J.Phys.Chem.B 113 Pages: 12865-12869
• [Journal Article] 近接場光学顕微鏡による高分子のナノ構造解析 (2009)
  • Author(s): 青木裕之
  • Journal Title: 高分子論文集 66 Pages: 312-320
  • NAID: 10025094206
• [Journal Article] Chain End Distribution of Block Copolymer in Two-dimensional Microphase-Separated Structure Studied by Scanning Near-Field Optical Microscopy (2009)
  • Author(s): R.Sekine, H.Aoki, S.Ito
  • Journal Title: J.Phys.Chem.B 113 Pages: 12865-12869
  • Peer Reviewed
• [Journal Article] Poly(methacrylate)s Labeled by Perylene Diimide : Synthesis and Applications in Single Chain Detection Studies (2009)
  • Author(s): H.Aoki, T.Takahashi, R.Sekine, Y.Tamai, S.Aoki, K.Tani, S.Ito
  • Journal Title: Polym.J. 41 Pages: 778-783
  • NAID: 10025525523
  • Peer Reviewed
• [Journal Article] Conformation of Single Homopolymer Chain in Micro-Phase Separated Block Copolymer Monolayer Studied by Scanning Near-Field Optical Microscopy (2009)
  • Author(s): Y.Tamai, R.Sekine, H.Aoki, S.Ito
  • Journal Title: Macromolecules 42 Pages: 4224-4229
  • Peer Reviewed
• [Journal Article] Affine Deformation of Single Polymer Chain in Poly(methyl methacrylate) Films under Uniaxial Extension Observed by Scanning Near-Field Optical Microscopy (2009)
  • Author(s): T.Ube, H.Aoki, S.Ito. J.Horinaka, T.Takigawa, T.Masuda
  • Journal Title: Polymer 50 Pages: 3016-3021
  • Peer Reviewed
• [Journal Article] Conformation of Single Block Copolymer Chain in Two-Dimensional Microphase-Separated Structure Studied by Scanning Near-Field Optical Microscopy (2009)
  • Author(s): R.Sekine, H.Aoki, S.Ito
  • Journal Title: J.Phys.Chem.B 113 Pages: 7095-7100
  • Peer Reviewed
• [Journal Article] Single-Molecular Imaging of Rotaxane Based on Glass Substrates : Observations of Rotary Movement of a Rotor (2008)
  • Author(s): Dai Nishimura, Yoshinori Takashima, Hiroyuki Aoki, Toshiaki Takahashi, Hiroyasu Yamaguchi, Shinzaburo Ito, Akira Harada
  • Journal Title: Angew. Chem. Int. Ed 120 Pages: 6166-6168
  • Peer Reviewed
• [Journal Article] Photo Gel-Sol/Sol-Gel Transition and Its Patterning of a Supramolecular Hydrogel as Stimuli-Responsive Biomaterials (2008)
  • Author(s): Shinji Matsumoto, Satoshi Yamaguchi, Shiori Ueno, Harunobu Komatsu, Masato Ikeda, Koji Ishizuka, Yuko Iko, Kazuhito V. Tabata, Hiroyuki Aoki, Shinzaburo Ito, Hiroyuki Noji. Raru Hamachi
  • Journal Title: Chem. Eur. J 14 Pages: 3977-3986
  • Peer Reviewed
• [Journal Article] 近接場光学顕微鏡による単一高分子鎖の延伸過程の直接観察 (2008)
  • Author(s): 青木裕之
  • Journal Title: ケミカルエンジニヤリング 53 Pages: 787-791
• [Presentation] 超解像光学による界面/薄膜における単一高分子鎖の構造評価 (2011)
  • Author(s): 青木裕之
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(招待講演)
  • Year and Date: 2011-03-26
• [Presentation] 単一分子の超解像観察による高分子鎖の構造解析 (2010)
  • Author(s): 森一樹, 青木裕之, 伊藤紳三郎
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 北海道大学
  • Year and Date: 2010-09-17
• [Presentation] 超解像光学顕微鏡による高分子材料の構造評価 (2010)
  • Author(s): 青木裕之, 森一樹
  • Organizer: 2010 年光化学討論会
  • Place of Presentation: 千葉大学
  • Year and Date: 2010-09-08
• [Presentation] Conformation of Single Polymer Chain under Tensile Deformation Studied by Near-Field Optical Microscopy (2010)
  • Author(s): Hiroyuki Aoki, Toru Ube, Shinzaburo Ito, Jun-ichi Horinaka
  • Organizer: International Symposium on Non-Equilibrium Soft Matter 2010
  • Place of Presentation: Nara
  • Year and Date: 2010-08-18
• [Presentation] Dynamics of Single Polymer Chain Studied by Defocus Fluorescence Imaging Technique (2010)
  • Author(s): Hiroyuki Aoki, Toshiaki Takahashi, Shinzaburo Ito
  • Organizer: 5th Pacific Rim Conference on Rheology (PRCR-5)
  • Place of Presentation: Sapporo
  • Year and Date: 2010-08-05
• [Presentation] Dynamics of Single Polymer Chain Studied by Defocus Fluorescence Imaging Technique (2010)
  • Author(s): Hiroyuki Aoki, Toshiaki Takahashi, Shinzaburo Ito
  • Organizer: 5th Pacific Rim Conference on Rheology
  • Place of Presentation: Sapporo
  • Year and Date: 2010-08-05
• [Presentation] Conformation of Single Polymer Chain under the Uniaxial Extension Studied by Scanning Near-Field Optical Microscopy (2010)
  • Author(s): Toru Ube, Hiroyuki Aoki, Shinzaburo Ito, Jun-ichi Horinaka
  • Organizer: 5th Pacific Rim Conference on Rheology (PRCR-5)
  • Place of Presentation: Sapporo
  • Year and Date: 2010-08-03
• [Presentation] Properties of single polymer chain studied by near-field/super-resolution optical microscopy (2010)
  • Author(s): H.Aoki
  • Organizer: 6th Single Molecule Meeting
  • Place of Presentation: 東京工業大
  • Year and Date: 2010-03-10
• [Presentation] 単一分子観察による高分子鎖の構造とダイナミクスの評価 (2010)
  • Author(s): 青木裕之
  • Organizer: 09-2高分子表面研究会
  • Place of Presentation: 東京理科大森戸記念館
  • Year and Date: 2010-01-15
• [Presentation] Conformation of single polymer chain in uniaxially stretched film studied by scanning near-field optical microscopy (2009)
  • Author(s): Toru Ube, Hiroyuki Aoki, Shinzaburo Ito, Jun-ichi Horinaka
  • Organizer: 11th Pacific Polymer Conference (PPC11)
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2009-12-08
• [Presentation] Conformation of single polymer chain in uniaxially stretched film studied by scanning near-field optical microscopy (2009)
  • Author(s): T.Ube, H.Aoki, S.Ito. J.Horinaka
  • Organizer: 11th Pacific Polymer Conference
  • Place of Presentation: ケアンズ(オーストラリア)
  • Year and Date: 2009-12-08
• [Presentation] Scanning near-field optical microscopy study for single homopolymer chain location and orientation in microphase-separated block copolymer monolayer (2009)
  • Author(s): Yasunari Yamai, Ryojun Sekine, Hiroyuki Aoki, Shinzaburo Ito
  • Organizer: 7th Asia-Pacific conference on Near-Field Optics (APNFO-7)
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2009-11-26
• [Presentation] Scanning near-field optical microscopy study for single homopolymer chain location and orientation in microphase-separated block copolymer monolayer (2009)
  • Author(s): Y.Tamai, R.Sekine, H.Aoki, S.Ito
  • Organizer: 7th Asia-Pacific conference on Near-Field Optics
  • Place of Presentation: 済州島(韓国)
  • Year and Date: 2009-11-26
• [Presentation] 近接場光学顕微鏡でみる絡み合い系における単一高分子鎖の伸長と緩和 (2009)
  • Author(s): 宇部達, 青木裕之, 伊藤紳三郎, 堀中順一
  • Organizer: 第57回レオロジー討論会
  • Place of Presentation: 宇部全日空ホテル, 山口
  • Year and Date: 2009-10-07
• [Presentation] 近接場光学顕微鏡でみる絡み合い系における単一高分子鎖の伸長と緩和 (2009)
  • Author(s): 宇部達, 青木裕之, 伊藤紳三郎, 堀中順一
  • Organizer: 第57回レオロジー討論会
  • Place of Presentation: 宇部全日空ホテル
  • Year and Date: 2009-10-07
• [Presentation] 超解像蛍光観察による単一高分子鎖の拡がりの解析 (2009)
  • Author(s): 青木裕之, 森一樹, 高橋利明, 伊藤紳三郎
  • Organizer: 第58回高分子討論会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-09-18
• [Presentation] Conformation of Single Poly(methyl methacrylate) Chains in an Ultra-Thin Film Studied by Scanning Near-Field Optical Microscopy (2009)
  • Author(s): 青木裕之
  • Organizer: 第58回高分子討論会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-09-18
• [Presentation] 近接場光学顕微鏡による高分子単分子膜のミクロ相分離構造における単一分子鎖の形態評価 (2009)
  • Author(s): 玉井康成, 關根良潤, 青木裕之, 伊藤紳三郎
  • Organizer: 第58回高分子討論会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-09-17
• [Presentation] 単一分子イメージングによるポリメタクリレート高分子鎖の構造評価 (2009)
  • Author(s): 青木裕之, 森一樹, 高橋利明, 伊藤紳三郎
  • Organizer: 2009年光化学討論会
  • Place of Presentation: 桐生市市民文化会館
  • Year and Date: 2009-09-16
• [Presentation] 近接場光学顕微鏡でみる絡み合い系における単一高分子鎖の緩和挙動 (2009)
  • Author(s): 宇部達, 青木裕之, 伊藤紳三郎, 堀中順一
  • Organizer: 第58回高分子討論会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-09-16
• [Presentation] 単一分子イメージングによるポリメタクリレート高分早鎖の構造評価 (2009)
  • Author(s): 青木裕之, 森一樹,高橋利明, 伊藤紳三郎
  • Organizer: 2009年光化学討論会
  • Place of Presentation: 桐生市市民文化会館
  • Year and Date: 2009-09-16
• [Presentation] 超解像光学観察による単一高分子鎖の構造とダイナミクスの評価 (2009)
  • Author(s): 青木裕之
  • Organizer: 第2回長崎ナノダイナミクス講演会
  • Place of Presentation: 長崎大学
  • Year and Date: 2009-03-14
• [Presentation] 固-液界面における高分子ブラシの分子運動 (2008)
  • Author(s): 青木裕之
  • Organizer: 08-1高分子表面研究会
  • Place of Presentation: 東京工業大学百年記念館
  • Year and Date: 2008-10-17
• [Presentation] 固一液界面における高分子ブラシの分子運動 (2008)
  • Author(s): 青木裕之
  • Organizer: 08-1高分子表面研究会
  • Place of Presentation: 東京工業大学百年記念館
  • Year and Date: 2008-10-17
• [Presentation] 近接場光学顕微鏡を用いたPMMA膜の伸長および緩和過程における単一高分子鎖の形態観察 (2008)
  • Author(s): 宇部達、青木裕之、伊藤紳三郎、堀中順一
  • Organizer: 第56回レオロジー討論会
  • Place of Presentation: 朱鷺メッセ
  • Year and Date: 2008-10-08
• [Presentation] 近接場光学顕微鏡でみるPMMA延伸膜中における単一高分子鎖の伸長および緩和挙動 (2008)
  • Author(s): 宇部達、青木裕之、伊藤紳三郎、堀中順一
  • Organizer: 第57回高分子討論会
  • Place of Presentation: 大阪市立大学
  • Year and Date: 2008-09-30
• [Presentation] 近接場光学顕微鏡による二次元ミクロ相分離構造中におけるブロックコポリマー鎖のコンホメーションに関する研究 (2008)
  • Author(s): 関根良潤、青木裕之、伊藤紳三郎
  • Organizer: 第57回高分子討論会
  • Place of Presentation: 大阪市立大学
  • Year and Date: 2008-09-30
• [Presentation] デンドリマー開始リビングラジカル重合法による19F-MRIプローブとしての含フッ素高分子ナノ微粒子 (2008)
  • Author(s): 小川倫弘、仁田原智、青木裕之、伊藤紳三郎、吉村公博、都築英寿、富田佳紀、矢野哲哉
  • Organizer: 第57回高分子討論会
  • Place of Presentation: 大阪市立大学
  • Year and Date: 2008-09-29
• [Presentation] ナノエマルジョン法によるFRET型高分子ナノ微粒子の作製と光イメージングへの応用 (2008)
  • Author(s): 角田純一、仁田原智、青木裕之、伊藤紳三郎、吉村公博、南昌人、富田佳紀、矢野哲哉
  • Organizer: 第57回高分子討論会
  • Place of Presentation: 大阪市立大学
  • Year and Date: 2008-09-29
• [Presentation] 単一分子配向イメージングによる高分子鎖ダイナミクスの評価 (2008)
  • Author(s): 高橋利明、青木裕之、伊藤紳三郎
  • Organizer: 第57回高分子討論会
  • Place of Presentation: 大阪市立大学
  • Year and Date: 2008-09-28
• [Presentation] 単一分子計測による高分子鎖の形態とダイナミクスの評価 (2008)
  • Author(s): 青木裕之
  • Organizer: 第55回関西レオロジー研究会例会
  • Place of Presentation: キャンパスプラザ京都
  • Year and Date: 2008-08-29
• [Presentation] Structure of Polymer Ultra-Thin Film Studied by Scanning Near-Field Optical Microscopy (2008)
  • Author(s): Hiroyuki Aoki
  • Organizer: Taiwan-Japan Bilateral Symposium on Surface/Interface Science and Nano-Soft Material
  • Place of Presentation: Taroko, Taiwan
  • Year and Date: 2008-08-09
• [Presentation] 近接場光学分光と単一分子分光によって明らかにする単一高分子鎖の構造とダイナミクス (2008)
  • Author(s): 青木裕之
  • Organizer: 第70回高分子若手研究会[関西]
  • Place of Presentation: 京都府立ゼミナールハウス
  • Year and Date: 2008-07-26
• [Presentation] 近接場光学顕微鏡による高分子単分子膜のミクロ相分離構造と分子鎖形態の評価 (2008)
  • Author(s): 玉井康成, 關根良潤, 青木裕之, 伊藤紳三郎
  • Organizer: 第54回高分子研究発表会(神戸)
  • Place of Presentation: 兵庫県中央労働センター
  • Year and Date: 2008-07-18
• [Presentation] 超解像光学による単一高分子の構造評価 (2008)
  • Author(s): 青木裕之
  • Organizer: 第54回高分子研究発表会(神戸)
  • Place of Presentation: 兵庫県中央労働センター
  • Year and Date: 2008-07-18
• [Presentation] 近接場光学顕微鏡による二次元ミクロ相分離中における単一ブロックコポリマー鎖の形態観察 (2008)
  • Author(s): 関根良潤, 青木裕之, 伊藤紳三郎
  • Organizer: 第57回高分子年次大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-05-29
• [Presentation] デンドリマー開始剤からのリビングラジカル重合法による含フッ素高分子ナノ微粒子の設計と特性解析 (2008)
  • Author(s): 小川倫弘、仁田原智、青木裕之、伊藤紳三郎、吉村公博、都築英寿、富田佳紀、矢野哲哉
  • Organizer: 第57回高分子年次大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-05-29
• [Presentation] ミニエマルジョン法による共役高分子ナノ微粒子の作製と光イメージングへの応用 (2008)
  • Author(s): 角田純一、仁田原智、青木裕之、伊藤紳三郎、吉村公博、南昌人、富田佳紀、矢野哲哉
  • Organizer: 第57回高分子年次大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-05-29
• [Presentation] 近接場光学顕微鏡でみる一軸伸長および応力緩和過程における単一PMMA鎖の形態 (2008)
  • Author(s): 宇部達, 青木裕之, 堀中順一, 伊藤紳三郎
  • Organizer: 第57回高分子年次大会
  • Place of Presentation: パシフィ"コ横浜
  • Year and Date: 2008-05-28
• [Presentation] 近接場光学顕微鏡でみる一軸伸長および応力緩和過程における単一PMMA鎖の形態 (2008)
  • Author(s): 宇部達、青木裕之、堀中順一、伊藤紳三郎
  • Organizer: 第57回高分子年次大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-05-28
• [Presentation] 近接場光学顕微鏡による一軸伸長下における単一高分子鎖の形態評価 (2008)
  • Author(s): 青木裕之、宇部達、伊藤紳三郎
  • Organizer: 日本顕微鏡学会第64回学術講演会
  • Place of Presentation: 京都国際会館
  • Year and Date: 2008-05-21
• [Book] 高分子分析入門(近接場光学顕微鏡)第16章 (2010)
  • Author(s): 青木裕之
  • Publisher: 講談社サイエンティフィク
• [Book] 近接場光のセンシング・イメージング技術への応用-最新のバイオ・化学・デバイス分野への展開-(近接場光学顕微鏡による高分子材料の構造評価)(民谷栄一, 朝日剛監修)第10章 (2010)
  • Author(s): 青木裕之
  • Publisher: シーエムシー出版
• [Book] 近接場光のセンシング・イメージング技術への応用-最新のバイオ・化学・デバイス分野への展開-(分担執筆) (2010)
  • Author(s): 青木裕之
  • Publisher: シーエムシー出版
• [Book] Molecular Nano Dynamics : Vol.I: Spectroscopic Methods and Nanostructures(Structure and Dynamics of Confined Polymer Chains Studied by Spatially and Temporally Resolved Fluorescence Techniques)(Ed.by H.Fukumura, M.Irie, Y.Iwasawa, M.Masuhara, K.Uosaki)Chap.4 (2009)
  • Author(s): Hiroyuki Aoki
  • Publisher: Wiley-VCH, Weinheim
• [Book] 高分子の表面・界面分析法の新展開(近接場光学顕微鏡による高分子薄膜・表面領域のナノ空間観察)(西岡利勝, 黒田孝二, 遠藤一央編) (2009)
  • Author(s): 青木裕之, 伊藤紳三郎
  • Publisher: シーエムシー出版
• [Remarks]
  • URL: http://photo.polym.kyoto-u.ac.jp/aoki/