Immunoprofiling of signaling molecule on malignant tumor

• Project/Area Number: 20689010
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Human pathology
• Research Institution: Hokkaido University
• Principal Investigator: NISHIHARA Hiroshi Hokkaido University, 大学院・医学研究科, 特任准教授 (50322805)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000); Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2008: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
• Keywords: 診断病理学 / 低分子量G蛋白 / Rac / 免疫染色 / シグナル伝達分子 / Immunoprofiling / Immumoprofiling

Research Abstract

The development of molecular targeted therapy for cancer treatment is necessary to establish the "Taylor made medicine", and also requires the personalized diagnostic system to evaluate the individual biological malignancy. In this study, we have performed the immunostain on the human cancer specimens for multiple signaling molecules, such as receptor tyrosine kinases and adaptor proteins, to establish the pathological basis for the personalized pathological diagnosis.

Research Products

• [Journal Article] DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma. (2010)
  • Author(s): Wang L, Nishihara H, Kimura T, Kato Y, Tanino M, Nishio M, Obara M, Endo T, Koike T, Tanaka S
  • Journal Title: Biochem Biophys Res Commun (Epub ahead of print)
  • NAID: 120002148671
  • Peer Reviewed
• [Journal Article] Analysis of an alternative human CD133 promoter reveals the implication of Ras/ERK pathway in tumor stem-like hallmarks. (2010)
  • Author(s): Tabu K, Kimura T, Sasai K, Wang L, Bizen N, Nishihara H, Taga T, Tanaka S
  • Journal Title: Mol Cancer 9(1) Pages: 39-39
  • Peer Reviewed
• [Journal Article] Sudden death of a patient with pandemic influenza (A/H1N1pdm) virus infection by acute respiratory distress syndrome. (2010)
  • Author(s): Takiyama A, Wang L, Tanino M, Kimura T, Kawagishi N, Kunieda Y, Katano H, Nakajima N, Hasegawa H, Takagi T, Nishihara H, Sata T, Tanaka S
  • Journal Title: Jpn J Infect Dis 63(1) Pages: 72-4
  • Peer Reviewed
• [Journal Article] A case of central nervous system lymphomatoid granulomatosis; characteristics of PET imaging and pathological findings. (2009)
  • Author(s): Nishihara H, Nakasato M, Sawa H, Murakami H, Yamamoto D, Moriyama K, Kato N, Hashimoto I, Kamada H, Tanaka S
  • Journal Title: J Neurooncol 93(2) Pages: 275-8
• [Journal Article] Human synovial sarcoma proto-oncogene Syt is essential for early embryonic development through the regulation of cell migration. (2009)
  • Author(s): Kimura T, Sakai M, Tabu K, Wang L, Tsunematsu R, Tsuda M, Sawa H, Nagashima K, Nishihara H, Hatakeyama S, Nakayama K, Ladanyi M, Tanaka S, Nakayama KI
  • Journal Title: Lab Invest 89(6) Pages: 645-56
  • Peer Reviewed
• [Journal Article] Crk adaptor protein-induced phosphorylation of Gab1 on tyrosine 307 via Src is important for organization of focal adhesions and enhanced cell migration. (2009)
  • Author(s): Watanabe T, Tsuda M, Makino Y, Konstantinou T, Nishihara H, Majima T, Minami A, Feller SM, Tanaka S
  • Journal Title: Cell Res. 19(5) Pages: 638-50
  • Peer Reviewed
• [Journal Article] DOCK2 regulates cell proliferation through Rae and ERK activation in B cell lymphoma
  • Author(s): Wang L, Nishihara H, et.al
  • Journal Title: Biochemical and Biophysical Research Communications (In press)
  • Peer Reviewed
• [Presentation] ヒト腫瘍間質・血管の実際 (2009)
  • Author(s): 西原広史
  • Organizer: 第25回日本DDS学会
  • Place of Presentation: 東京 東京ドームホテル
  • Year and Date: 2009-07-03
• [Presentation] Clinicopathological analysis for glioma-specific vascular structures. (2009)
  • Author(s): Hiroshi Nishihara, Mitsunobu R. Kano, Hiromi Kanno, Shinya Tanaka
  • Organizer: AACR Special Conference on Genetics and Biology of Brain Cancers
  • Place of Presentation: アメリカ、サンディエゴ
• [Presentation] CNS-lymphomatoid granulomatosis; as a primary lesion for CNS-T cell lymphoma (2009)
  • Author(s): Hiroshi Nishihara, Kazuo Nagashima, Shinya Tanaka
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] MDS患者のリンパ節に発生したtrilineage extramedullary myeloid cell tumorの1例 (2009)
  • Author(s): 西原広史、高阪真路、久保田佳奈子、田中伸哉、松野吉宏
  • Organizer: 第49回日本リンパ網内系学会総会
  • Place of Presentation: 淡路島
• [Presentation] Pathological analysis for cancer-mesenchymal and vascular structure associated with drug delivery system. (2009)
  • Author(s): 西原広史
  • Organizer: 第25回日本DDS学会学術集会ワークショップDDSとがん組織(間質、脈管)
  • Place of Presentation: 東京
• [Presentation] 髄膜腫におけるDOCK180の発現の検討 (2009)
  • Author(s): 西原広史、村上普美、木村憲一、佐和弘基、鎌田一、田中伸哉
  • Organizer: 第50回日本神経病理学会学術研究会
  • Place of Presentation: 香川
• [Presentation] 脳腫瘍血管壁の組織病理学的解析:Glomeruloid vesselは肥厚した幼若なPericyteで構成されている (2009)
  • Author(s): 西原広史、狩野光伸、田中伸哉
  • Organizer: 第27回日本脳腫瘍病理学会総会
  • Place of Presentation: 福岡
• [Presentation] Pilomyxoid patternを示すAnaplastic oligoastrocytoma (AOA)の病理学的検討 (2008)
  • Author(s): 西原広史、長嶋和郎、田中伸哉
  • Organizer: 第26回日本脳腫瘍病理学会総会
  • Place of Presentation: 東京
• [Presentation] Paul A. Insel. Cyclic AMP-dependent regulation of colon cancer growth and apoptosis: A key role for COX-2 (2008)
  • Author(s): Hiroshi Nishihara, Hiroshi Yokouchi, Shinya Tanaka, Lars Eckmann
  • Organizer: AACR (American Society of Cancer Research)
  • Place of Presentation: アメリカ、サンディエゴ