Quantitative immunoassay using magnetic detection for rapid and high sensitive clinical biochemistry

• Project/Area Number: 20689013
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Nagasaki International University
• Principal Investigator: KUMA Hiroyuki Nagasaki International University, 薬学部, 講師 (40435136)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000); Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: 磁気検出法 / 磁気ナノマーカー / 臨床検査 / 免疫検査 / ナノデバイス

Research Abstract

Biological immunoassay is one main detection system used in the field of clinical chemistry. In this study, we developed a new detection method utilizing a magnetic marker and magnetic sensor has enabled rapid and sensitive. Also, we demonstrated the ability for markers to measure biological targets. This detection system has great potential for use as the next generation's analytical system.

Research Products

• [Journal Article] 深部静脈血栓症とプロテインS異常症 (2011)
  • Author(s): 濱崎直孝, 隈博幸
  • Journal Title: 臨床検査 Volume: 55(4) Pages: 357-365
• [Journal Article] Liquid Phase Immunoassays utilizing magnetic marker and SQUID magneto-meter (2010)
  • Author(s): H.Kuma, H.Oyamada, A.Tsukamoto, T.Mizoguchi, A.Kandori, Y.Sugiura, K.Yoshinaga, K.Enpuku, N.Hamasaki
  • Journal Title: Clin.Chem.Lab.Med. 48(9) Pages: 1263-1269
  • Peer Reviewed
• [Journal Article] Liquid phase immunoassays utilizing magnetic marker and SQUID magnetometer (2010)
  • Author(s): Kuma H, et al.
  • Journal Title: Clin.Chem.Lab.Med. Volume: 48(9) Pages: 1263-1269
  • Peer Reviewed
• [Journal Article] Development of a SQUID System Using Field Reversal for Rapiddly Detecting Bacteria (2009)
  • Author(s): A.Tsukamoto, T.Mizoguchi, A.Kandori, H.Kuma, N.Hamasaki, H.Kanzaki, N.Usuki, K.Yoshinaga, K.Enpuku
  • Journal Title: IEEE Trans.Appl.Supercond. 19(3) Pages: 853-856
  • Peer Reviewed
• [Journal Article] Fast Detection of Biological Targets with Magnetic Marker and SQUID (2009)
  • Author(s): K.Enpuku, H.Tokumitsu, Y.Sugimoto, H.Kuma, N.Hamasaki, A.Tsukamoto, T.Mizoguchi, A.Kandori, K.Yoshinaga, H.Kanzaki, N.Usuki
  • Journal Title: IEEE Trans.Appl.Supercond. 19(3) Pages: 844-847
  • Peer Reviewed
• [Journal Article] Liquid-Phase Detection of Biological Targets with Magnetic Marker and Superconducting Quantum Interference Device (2009)
  • Author(s): K.Enpuku, Y.Sugimoto, Y.Tamai, A.Tsukamoto, T.Mizoguchi, A.Kandori, N.Usuki, H.Kanzaki, K.Yoshinaga, Y.Sugiura, H.Kuma, N.Hamasaki
  • Journal Title: IEICE Trans.on Electronics E92C(3) Pages: 315-322
  • NAID: 10026821319
  • Peer Reviewed
• [Journal Article] 磁気マーカーを用いた免疫検査システム (2009)
  • Author(s): 隈博幸, 円福敬二, 濱崎直孝
  • Journal Title: 検査と技術 37(2) Pages: 174-177
  • NAID: 130003357057
• [Journal Article] Fast Detection of Biological Targets with Magnetic Marker and SQUID (2009)
  • Author(s): Enpuku K, et al.
  • Journal Title: IEEE Trans.Appl.Supercond 19 Pages: 844-847
  • Peer Reviewed
• [Journal Article] Development of a SQUID System Using Field Reversal for Rapiddly Detecting Bacteria (2009)
  • Author(s): Tsukamoto A, et al.
  • Journal Title: IEEE Trans.Appl.Supercond 19 Pages: 853-856
  • Peer Reviewed
• [Journal Article] 磁気マーカーを用いた免疫検査システム (2009)
  • Author(s): 隈博幸ら
  • Journal Title: 検査と技術 37 Pages: 174-177
  • NAID: 130003357057
  • Peer Reviewed
• [Journal Article] Liquid-Phase Detection of Biological Targets with Magnetic Marker and Superconducting Quantum Interference Device (2009)
  • Author(s): Enpuku K. et.al.
  • Journal Title: IEICE Trans. Electron E92-C Pages: 315-322
  • Peer Reviewed
• [Journal Article] Liquid phase immunoassays utilizing magnetic marker and SQUID magnetometer
  • Author(s): Kuma H, et al.
  • Journal Title: Clin.Chem.Lab.Med. (掲載確定)
  • Peer Reviewed
• [Presentation] 磁気検出法を用いた高感度な免疫検査技術の開発 (2010)
  • Author(s): 隈博幸
  • Organizer: 第32回日本薬学会九州支部コロキウム
  • Place of Presentation: 長崎国際大学(長崎県)
  • Year and Date: 2010-10-23
• [Presentation] 赤血球膜蛋白質バンド3の機能と構造 (2010)
  • Author(s): 隈博幸
  • Organizer: 第4回トランスポーター研究会九州部会
  • Place of Presentation: ハウステンボス(長崎県)
  • Year and Date: 2010-09-11
• [Presentation] 磁気検出法を用いた高感度な免疫検査技術の開発 (2010)
  • Author(s): 隈博幸
  • Organizer: 第32回日本薬学会九州支部コロキウム
  • Place of Presentation: 招待講演
• [Presentation] 磁気検出法による定量的免疫検査システム (2009)
  • Author(s): 隈博幸、濱崎直孝
  • Organizer: 第49回 日本臨床化学会 年次学術集会
  • Place of Presentation: 長崎大学医学部
  • Year and Date: 2009-09-19
• [Presentation] 磁気検出法による定量的免疫検査システム (2009)
  • Author(s): 隈博幸, 濱崎直孝
  • Organizer: 第49回日本臨床化学会年次学術集会
• [Presentation] Fast detection of biological targets with magnetic marker and SQUID (2008)
  • Author(s): K Enpuku, H.Tokumitsu, Y.Sugimoto, H.Kuma, N.Hamasaki, A.Tsukamoto, T.Mizoguchi, A.Kandori, H.Kanzaki, N.Usuki
  • Organizer: Applied Superconductivity Conference (ASC)
• [Remarks] ホームページ(長崎国際大学薬学部薬学科・臨床検査学研究室)
  • URL: http://www.niu.ac.jp/~pharm1/lab/cclm/index.html
• [Remarks]
  • URL: http://www.niu.ac.jp/~pharml/lab/cclm/investigation.html