Monitoring of CREB phosphorylation in single live neuron using split luciferase

• Project/Area Number: 20700331
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: University of Toyama
• Principal Investigator: ISHIMOTO Tetsuya University of Toyama, 大学院・医学薬学研究部(医学), 助教 (40397170)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: CREB / リン酸化 / ルシフェラーゼ / イメージング

Research Abstract

cAMP response element binding protein (CREB) is a transcription factor which is activated by phosphorylation. We developed probe proteins that detected CREB phosphorylation using bioluminescence. In addition to the 1-molecule-type probe that was planned at the beginning of the project, we made 2-molecule-type probes. This approach increased the specificity of the probes.

Research Products

• [Journal Article] Real-time monitoring of actin polymerization in living cells using split luciferase.
  • Author(s): Ishimoto T, Ozawa T, Mori H.
  • Journal Title: Bioconjugate Chemistry in press
  • Peer Reviewed
• [Journal Article] Bioluminescence imaging of Arc expression enables detection of activity-dependent and plastic changes in the visual cortex of adult mice.
  • Author(s): Izumi H, Ishimoto T, Yamamoto H, Nishijo H, Mori H.
  • Journal Title: Brain Struct Funct. in press
  • Peer Reviewed
• [Presentation] Actin polymerization and its role in UV-induced HEK293T cell death. (2011)
  • Author(s): 石本哲也, 小澤岳昌, 森寿
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-24
• [Presentation] Actin polymerization and its role in UV-induced HEK293T cell death (2011)
  • Author(s): 石本哲也, 小澤岳昌, 森寿
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: (誌上発表(学会中止のため))
  • Year and Date: 2011-03-24
• [Presentation] Bioluminescence imaging of Arc gene expression detects neural activity-dependent changes in vivo. (2010)
  • Author(s): 和泉宏謙, 石本哲也, 山本博, 西条寿夫, 森寿
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
• [Presentation] Real-time measurement of actin polymerization in live cells : Transient upregulation in F-actin after UV irradiation and its protective effect on cell death. (2010)
  • Author(s): 石本哲也, 小澤岳昌, 森寿
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
• [Presentation] Real-time measurement of F-actin content during cell death using a bioluminescence based method. (2009)
  • Author(s): 石本哲也, 和泉宏謙, 小澤岳昌, 森寿
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Real-time measurement of F-actin content during cell death using a bioluminescence based method (2009)
  • Author(s): 石本哲也, 和泉宏謙, 小澤岳昌, 森寿
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Detection of protein-protein interaction in living cells using a bioluminescence based method. (2009)
  • Author(s): Ishimoto T., Izumi H., Ozawa T., Mori H.
  • Organizer: The 40th Annual Meeting of Society for Neuroscience
  • Place of Presentation: Chicago, USA.
  • Year and Date: 2009-10-19
• [Presentation] Detection of protein-protein interaction in living cells using a bioluminescence based method (2009)
  • Author(s): Ishimoto T., Izumi H., Ozawa T., Mori H
  • Organizer: The 40th Annual Meeting of Society for Neuroscience
  • Place of Presentation: Chicago, USA
  • Year and Date: 2009-10-19
• [Presentation] 生物発光を利用した細胞内アクチン重合の計測 (2009)
  • Author(s): 石本哲也, 和泉宏謙, 小澤岳昌, 森寿
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] スプリットルシフェラーゼを用いたアクチン重合の検出 (2009)
  • Author(s): 石本哲也、和泉宏謙、小澤岳昌、森寿
  • Organizer: 日本薬理学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-16
• [Presentation] スプリットルシフェラーゼを用いたアクチン重合の検出 (2008)
  • Author(s): 石本哲也, 和泉宏謙, 小澤岳昌, 森寿
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-16
• [Patent(Industrial Property Rights)] 神経活動を可視化するプローブ (2009)
  • Inventor(s): 石本哲也, 森寿, 和泉宏謙
  • Industrial Property Rights Holder: 富山大学
  • Filing Date: 2009-08-12
  • Overseas
• [Patent(Industrial Property Rights)] 神経活動を可視化するプローブ (2009)
  • Inventor(s): 石本哲也、森寿、和泉宏謙
  • Industrial Property Rights Holder: 富山大学
  • Filing Date: 2009-08-12
  • Overseas
• [Patent(Industrial Property Rights)] 記憶形成の神経活動を可視化するプローブ (2008)
  • Inventor(s): 石本哲也, 森寿, 和泉宏謙
  • Industrial Property Rights Holder: 富山大学
  • Industrial Property Number: 2008-207546
  • Filing Date: 2008-08-12
• [Patent(Industrial Property Rights)] 記憶形成の神経活動を可視化するプローブ (2008)
  • Inventor(s): 石本哲也、森寿、和泉宏謙
  • Industrial Property Rights Holder: 富山大学
  • Industrial Property Number: 2008-207546
  • Filing Date: 2008-08-12